AB52955

Anti-TGIF antibody [EP637Y]

RabMAb
Recombinant
What is this?

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(10 Publications)

Rabbit Recombinant Monoclonal TGIF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 10 publications.

View Alternative Names

TGIF, TGIF1, Homeobox protein TGIF1, 5'-TG-3'-interacting factor 1

3 Images

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Immunoprecipitation - Anti-TGIF antibody [EP637Y] (AB52955)

ab52955 (purified) at 1 : 100 dilution (2μg) immunoprecipitating TGIF in HeLa whole cell lysate.
Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10μg
Lane 2 (+) : ab52955 & HeLa whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab52955 in HeLa whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TGIF antibody [EP637Y] (ab52955)

Predicted band size: 43 kDa

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Western blot - Anti-TGIF antibody [EP637Y] (AB52955)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-TGIF antibody [EP637Y] (ab52955) at 0.4 µg/mL

All lanes:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 43 kDa

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Unknown

Western blot - Anti-TGIF antibody [EP637Y] (AB52955)

All lanes:

Western blot - Anti-TGIF antibody [EP637Y] (ab52955) at 1/1000 dilution

All lanes:

K562 cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa

Observed band size: 30 kDa

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Carrier free

Anti-TGIF antibody [EP637Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP637Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab52955 detects both phosphorylated and unphosphorylated TGIF.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100", "IP-species-notes": "For unpurified use at 1/30", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "For unpurified use at 1/20000", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds to a retinoid X receptor (RXR) responsive element from the cellular retinol-binding protein II promoter (CRBPII-RXRE). Inhibits the 9-cis-retinoic acid-dependent RXR alpha transcription activation of the retinoic acid responsive element. Active transcriptional corepressor of SMAD2. Links the nodal signaling pathway to the bifurcation of the forebrain and the establishment of ventral midline structures. May participate in the transmission of nuclear signals during development and in the adult, as illustrated by the down-modulation of the RXR alpha activities.

See full target information TGIF1

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Chronic obstructive pulmonary diseases (Miami, Fla.) 12:285-293 PubMed40338977

2025

The Role of in Regulating Airway Immune Dysfunction in COPD Through the Transforming Growth Factor-Beta/Polymeric Immunoglobulin Receptor Pathway: An In Vitro Study.

Applications

Unspecified application

Species

Unspecified reactive species

Hu Liu,Yun Zhao,Jing Cao,Lei Liang,Jinmeng Zhou

eLife 13: PubMed38661167

2024

Tgif1-deficiency impairs cytoskeletal architecture in osteoblasts by activating PAK3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Simona Bolamperti,Hiroaki Saito,Sarah Heerdmann,Eric Hesse,Hanna Taipaleenmäki

Cancer cell international 22:191 PubMed35578338

2022

Silencing of histone deacetylase 3 suppresses the development of esophageal squamous cell carcinoma through regulation of miR-494-mediated TGIF1.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Yuan Zhang,Zongxiang Lin,Kai Wu,Zhanfeng He,Dengyan Zhu,Jia Zhao,Chunyang Zhang,Yuxia Fan

Oncology reports 47: PubMed34878149

2021

CtBP2 interacts with TGIF to promote the progression of esophageal squamous cell cancer through the Wnt/β‑catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qianqian Ju,Maorong Jiang,Wenxin Huang,Qingbo Yang,Zhenghong Luo,Hui Shi

Cancer management and research 13:2603-2612 PubMed33776478

2021

TGIF1 Knockdown Inhibits the Proliferation and Invasion of Gastric Cancer via AKT Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhang,Feiyan Zhang,Jiye Fan,Bin Feng

Oncogenesis 9:92 PubMed33070167

2020

Aberrant super-enhancer landscape reveals core transcriptional regulatory circuitry in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Te Zhang,Xuming Song,Zeyu Zhang,Qixing Mao,Wenjie Xia,Lin Xu,Feng Jiang,Gaochao Dong

Cancer management and research 12:5677-5687 PubMed32765071

2020

The Chinese Medicine, Shezhi Huangling Decoction, Inhibits the Growth and Metastasis of Glioma Cells via the Regulation of miR-1298-5p/TGIF1 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqian Liu,Jianfeng Ju,Qun Liu,Zongmin Zhu,Chunxia Liu

Breast cancer research : BCR 22:34 PubMed32272947

2020

Breast cancer bone metastases are attenuated in a Tgif1-deficient bone microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Therese Haider,Hiroaki Saito,Jennifer Zarrer,Kevin Uzhunnumpuram,Sankari Nagarajan,Vijayalakshmi Kari,Michael Horn-Glander,Stefan Werner,Eric Hesse,Hanna Taipaleenmäki

Nature communications 10:1354 PubMed30902975

2019

TG-interacting factor 1 (Tgif1)-deficiency attenuates bone remodeling and blunts the anabolic response to parathyroid hormone.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroaki Saito,Andreas Gasser,Simona Bolamperti,Miki Maeda,Levi Matthies,Katharina Jähn,Courtney L Long,Hartmut Schlüter,Marcel Kwiatkowski,Vaibhav Saini,Paola Divieti Pajevic,Teresita Bellido,Andre J van Wijnen,Khalid S Mohammad,Theresa A Guise,Hanna Taipaleenmäki,Eric Hesse

Fertility and sterility 94:1078-83 PubMed19524896

2009

Transforming growth interacting factor expression in leiomyoma compared with myometrium.

Applications

Species

Unspecified reactive species

Jason Yen-Ping Ho,Weng Chi Man,Yan Wen,Mary Lake Polan,Esther Shih-Chu Ho,Bertha Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TGIF antibody [EP637Y]

Immunoprecipitation - Anti-TGIF antibody [EP637Y] (AB52955)

Western blot - Anti-TGIF antibody [EP637Y] (AB52955)

Western blot - Anti-TGIF antibody [EP637Y] (AB52955)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (10)

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