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Rabbit Recombinant Monoclonal TGON3 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Rat samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN38 antibody [EPR28143-541] (AB321791), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN38 antibody [EPR28143-541] (AB321791), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN38 antibody [EPR28143-541] (AB321791), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TGN38 antibody [EPR28143-541] (AB321791), expandable thumbnail
  • Western blot - Anti-TGN38 antibody [EPR28143-541] (AB321791), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-PIP
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Tested
Tested
Not recommended

Tested
Tested

Species
Rat
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/5000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Target data

Alternative names

Trans-Golgi network integral membrane protein TGN38

Rabbit Recombinant Monoclonal TGON3 antibody. Suitable for ICC/IF, WB, IHC-P and reacts with Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28143-541
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TGN38 also known as trans-Golgi network integral membrane protein 2 is a protein with a mass of approximately 38 kDa. It plays a role in the sorting and trafficking of proteins within the trans-Golgi network which acts as a hub for protein sorting and distribution in cells. It gets expressed mostly in the trans-Golgi network of various mammalian cells. TGN38 serves as a receptor that helps in the selection and distribution of proteins to their respective destinations maintaining intracellular protein transport efficiency.

Biological function summary

TGN38 contributes to the transport and stability of proteins within the Golgi apparatus though it does not form any known complex. The protein is involved in the retention and recycling of resident Golgi proteins helping maintain the functionality of the Golgi network. TGN38 coordinates with other cellular components to regulate the movement of proteins through secretory pathways which is essential for proper cellular function and protein distribution processes.

Pathways

TGN38 participates in the vesicular transport pathway and cisternal maturation models. It links to proteins like coated vesicle proteins and SNAREs that assist in the formation and movement of vesicles carrying proteins through cellular compartments. TGN38 helps ensure that proteins reach their correct destination interacting with these pathways to maintain efficient transport and secretion within the cell.

Associated diseases and disorders

TGN38 shows connections with conditions related to protein trafficking and secretion defects such as hereditary spastic paraplegia. Aberrant function or expression of TGN38 can disrupt normal protein sorting contributing to these disorders. In particular it interacts with proteins like atlastin-1 which are also involved in maintaining the integrity of intracellular trafficking and are implicated in the pathogenesis of some neurological disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN38 antibody [EPR28143-541] (ab321791), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN38 antibody [EPR28143-541] (ab321791)

    Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling TGN38 with ab321791 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat kidney. The section was incubated with ab321791 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN38 antibody [EPR28143-541] (ab321791), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN38 antibody [EPR28143-541] (ab321791)

    Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling TGN38 with ab321791 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat spleen. The section was incubated with ab321791 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN38 antibody [EPR28143-541] (ab321791), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN38 antibody [EPR28143-541] (ab321791)

    Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling TGN38 with ab321791 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on rat cerebrum. The section was incubated with ab321791 for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

  • Immunocytochemistry/ Immunofluorescence - Anti-TGN38 antibody [EPR28143-541] (ab321791), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TGN38 antibody [EPR28143-541] (ab321791)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RBL-2H3 (rat Peripheral blood basophil) cells labelling TGN38 with ab321791 at 1/50 (10.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing golgi staining in RBL-2H3 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-GM130 antibody - BSA and Azide free ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain tubulin at 1/200 5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

    -ve control 1: ab321791 at 1/50 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
    -ve control 2: Anti-GM130 antibody - BSA and Azide free ab169276 at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution.

  • Western blot - Anti-TGN38 antibody [EPR28143-541] (ab321791), expandable thumbnail

    Western blot - Anti-TGN38 antibody [EPR28143-541] (ab321791)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profiles/observed MW are consistent with what has been described in the literature (PMID: 12494998).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lanes 1-2: 91 seconds; Lane 3: 136 seconds

    All lanes: Western blot - Anti-TGN38 antibody [EPR28143-541] (ab321791) at 1/1000 dilution

    Lane 1: RBL-2H3 (rat Peripheral blood basophil) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: RBL-2H3 transfected with siRNA specifically targeting TGN38 whole cell lysate at 20 µg

    Lane 3: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 90 kDa, 36 kDa

  • Western blot - Anti-TGN38 antibody [EPR28143-541] (ab321791), expandable thumbnail

    Western blot - Anti-TGN38 antibody [EPR28143-541] (ab321791)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profiles/observed MW are consistent with what has been described in the literature (PMID: 12494998).

    The identity of the lower MW band at approximately 60kDa is unknown.

    The lysates are suggested freshly made and used for Western Blotting immediately to minimize protein degradation

    All lanes: Western blot - Anti-TGN38 antibody [EPR28143-541] (ab321791) at 1/1000 dilution

    Lane 1: Rat testis tissue lysate at 20 µg

    Lane 2: Rat spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 90 kDa

    Exposure time: 37s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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