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Rabbit Recombinant Monoclonal TGN46 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 2 publications.

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Images

Western blot - Anti-TGN46 antibody [EPR12676] - Golgi Marker (AB174280), expandable thumbnail
  • Immunoprecipitation - Anti-TGN46 antibody [EPR12676] - Golgi Marker (AB174280), expandable thumbnail
  • Western blot - Anti-TGN46 antibody [EPR12676] - Golgi Marker (AB174280), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (AB174280), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (AB174280), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFIPFlow CytWB
Human
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/50 - 1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/5000
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

May be involved in regulating membrane traffic to and from trans-Golgi network.

Alternative names

TGN46, TGN51, TGOLN2, Trans-Golgi network integral membrane protein 2, Trans-Golgi network glycoprotein 46, Trans-Golgi network glycoprotein 48, Trans-Golgi network glycoprotein 51, Trans-Golgi network protein 2, TGN38 homolog, hTGN46, hTGN48, hTGN51

Recommended products

Rabbit Recombinant Monoclonal TGN46 antibody. Suitable for IHC-P, ICC/IF, IP, WB and reacts with Human samples. Cited in 2 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR12676
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TGN46 also known as trans-Golgi network protein 46 is an important membrane protein associated with the trans-Golgi network. The protein has a mass of approximately 46 kDa and plays a role in the sorting and trafficking of proteins to their proper destinations within the cell. TGN46 is widely expressed in various cell types and is commonly used as a Golgi marker in cell biology studies helping to identify Golgi apparatus in cells.

Biological function summary

The protein TGN46 functions in the sorting and transport mechanisms within the cell. It does not function as a part of a large macromolecular complex but it interacts with several other proteins to facilitate the movement of proteins from the Golgi to other sites within the cell. The protein also associates with trafficking components to ensure the correct delivery of proteins to the plasma membrane or lysosomes.

Pathways

The role of TGN46 involves participation in intracellular transport and protein trafficking pathways. It interacts with clathrin-coated vesicles and works closely with proteins such as gamma-adaptin and epsin which are important for vesicle formation and transport. These interactions highlight its involvement in protein secretion and membrane transport pathways which are essential for maintaining cellular homeostasis.

Associated diseases and disorders

TGN46 appears to have connections to neurodegenerative diseases and certain forms of cancer although the mechanisms are not fully understood. Alterations in TGN46 have been observed in Alzheimer's disease where it may intersect with proteins like amyloid precursor protein (APP) in protein misfolding and aggregation pathways. Additionally changes in TGN46 expression levels have been implicated in prostate cancer potentially due to its role in protein sorting and dysregulation of cellular trafficking processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-TGN46 antibody [EPR12676] - Golgi Marker (ab174280), expandable thumbnail

    Western blot - Anti-TGN46 antibody [EPR12676] - Golgi Marker (ab174280)

    TGN46 Western blot staining using rabbit Anti-TGN46 antibody

    Lanes 1 - 4: Merged signal (red and green). Green - ab174280 observed at 80 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

    ab174280 was shown to react with TGN46 in wild-type A549 cells in Western blot with loss of signal observed in TGOLN2 knockout cell line Human TGOLN2 (TGN46) knockout A549 cell line ab269510 (knockout cell lysate Human TGOLN2 (TGN46) knockout A549 cell lysate ab269672). Wild-type A549 and TGOLN2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab174280 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-TGN46 antibody [EPR12676] - Golgi Marker (ab174280) at 1/1000 dilution

    Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: TGOLN2 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg

    Lane 2: Western blot - Human TGOLN2 (TGN46) knockout A549 cell line (Human TGOLN2 (TGN46) knockout A549 cell line ab269510)

    Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Hep G2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 51 kDa

    Observed band size: 80 kDa

  • Immunoprecipitation - Anti-TGN46 antibody [EPR12676] - Golgi Marker (ab174280), expandable thumbnail

    Immunoprecipitation - Anti-TGN46 antibody [EPR12676] - Golgi Marker (ab174280)

    Immunoprecipitation. ab174280 at 1/1000 labeling TGN46 immunoprecipitated from Human fetal liver lysate using ab174280 at 1/10.

    All lanes: Immunoprecipitation - Anti-TGN46 antibody [EPR12676] - Golgi Marker (ab174280)

    Predicted band size: 51 kDa

  • Western blot - Anti-TGN46 antibody [EPR12676] - Golgi Marker (ab174280), expandable thumbnail

    Western blot - Anti-TGN46 antibody [EPR12676] - Golgi Marker (ab174280)

    TGN46 Western blot staining using rabbit Anti-TGN46 antibody

    Blocking buffer: 5% NFDM/TBST.

    Exposure: 10 seconds, 100 seconds.

    All lanes: Western blot - Anti-TGN46 antibody [EPR12676] - Golgi Marker (ab174280) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 51 kDa

    Observed band size: 75-100 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280)

    ab174280 staining TGN46 in wild-type A549 cells (top panel) and TGOLN2 knockout A549 cells (bottom panel) (Human TGOLN2 (TGN46) knockout A549 cell line ab269510). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab174280 at 1μg/ml concentration and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)

    Immunohistochemical analysis of paraffin embedded Human brain tissue labeling TGN46 with ab174280 at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] (ab174280)

    Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling TGN46 with ab174280 at 1/50.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR12676] (ab174280)

    ab174280 staining TGN46 in HepG2 cells (top panel - positive control) and HeLa cells (bottom panel - negative control, low TGN46 expression). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab174280 at 1μg/ml concentration and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

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