Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (ab271183)

Rabbit Recombinant Monoclonal TGN46 antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (AB271183), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/30	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/2000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Target data

See full target information TGOLN2

Function

May be involved in regulating membrane traffic to and from trans-Golgi network.

Alternative names

TGN46, TGN51, TGOLN2, Trans-Golgi network integral membrane protein 2, Trans-Golgi network glycoprotein 46, Trans-Golgi network glycoprotein 48, Trans-Golgi network glycoprotein 51, Trans-Golgi network protein 2, TGN38 homolog, hTGN46, hTGN48, hTGN51

Recommended products

Rabbit Recombinant Monoclonal TGN46 antibody. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR24770-16
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TGN46 also known as trans-Golgi network protein 46 is an important membrane protein associated with the trans-Golgi network. The protein has a mass of approximately 46 kDa and plays a role in the sorting and trafficking of proteins to their proper destinations within the cell. TGN46 is widely expressed in various cell types and is commonly used as a Golgi marker in cell biology studies helping to identify Golgi apparatus in cells.

Biological function summary

The protein TGN46 functions in the sorting and transport mechanisms within the cell. It does not function as a part of a large macromolecular complex but it interacts with several other proteins to facilitate the movement of proteins from the Golgi to other sites within the cell. The protein also associates with trafficking components to ensure the correct delivery of proteins to the plasma membrane or lysosomes.

Pathways

The role of TGN46 involves participation in intracellular transport and protein trafficking pathways. It interacts with clathrin-coated vesicles and works closely with proteins such as gamma-adaptin and epsin which are important for vesicle formation and transport. These interactions highlight its involvement in protein secretion and membrane transport pathways which are essential for maintaining cellular homeostasis.

Associated diseases and disorders

TGN46 appears to have connections to neurodegenerative diseases and certain forms of cancer although the mechanisms are not fully understood. Alterations in TGN46 have been observed in Alzheimer's disease where it may intersect with proteins like amyloid precursor protein (APP) in protein misfolding and aggregation pathways. Additionally changes in TGN46 expression levels have been implicated in prostate cancer potentially due to its role in protein sorting and dysregulation of cellular trafficking processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

Western blot - Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (ab271183)
TGN46 Western blot staining using rabbit Anti-TGN46 antibody
Blocking and diluting buffer and concentration: 3% NFDM/TBST.
Lanes 1-3: Merged signal (red and green).
Green: ab271183 observed at 80-100 kDa.
Red: loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse monoclonal [6C5] to GAPDH) observed at 36 kDa.
Lanes 1-2: ab271183 was shown to react with TGN46 in wild-type A549 cells in Western blot with loss of signal observed in TGN46 knockout cell line Human TGOLN2 (TGN46) knockout A549 cell line ab269510 (knockout cell lysate Human TGOLN2 (TGN46) knockout A549 cell lysate ab269672). Wild-type A549 and TGN46 knockout cell lysates were subjected to SDS-PAGE.
ab271183 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at 4°C overnight at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (ab271183) at 1/1000 dilution
Lane 1: Wild-type A549 (human lung carcinoma epithelial cell) whole cell lysate at 40 µg
Lane 2: TGOLN2 (TGN46) knockout A549 (human lung carcinoma epithelial cell) whole cell lysate at 40 µg
Lane 2: Western blot - Human TGOLN2 (TGN46) knockout A549 cell line (Human TGOLN2 (TGN46) knockout A549 cell line ab269510)
Lane 3: HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at a 1/10000 dilution.
Predicted band size: 51 kDa
Observed band size: 80-100 kDa
Western blot - Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (ab271183)
TGN46 Western blot staining using rabbit Anti-TGN46 antibody
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID:15616480; PMID: 22909819).
Lysates were made freshly and used in WB immediately to minimize protein degradation.
Exposure time: Lane 1: 26 seconds; Lane 2: 125 seconds.
All lanes: Western blot - Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (ab271183) at 1/1000 dilution
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 51 kDa
Observed band size: 80-100 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR24770-16] (ab271183)
Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling TGN46 with ab271183 at 1/2000 (0.297 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human cerebrum. The section was incubated with ab271183 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR24770-16] (ab271183)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling TGN46 with ab271183 at 1/2000 (0.297 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human kidney. The section was incubated with ab271183 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR24770-16] (ab271183)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling TGN46 with ab271183 at 1/2000 (0.297 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human colon. The section was incubated with ab271183 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunoprecipitation - Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (ab271183)
TGN46 was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug with ab271183 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271183 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 μg
Lane 2: HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab271183 in HepG2 whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 50 seconds
Lysates were made freshly and used in IP immediately to minimize protein degradation. Lower bands observed in lane 2 may caused by degradation during incubation based on the WB results.
All lanes: Immunoprecipitation - Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (ab271183)
Predicted band size: 51 kDa
Observed band size: 80-100 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR24770-16] (ab271183)
Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labelling TGN46 with ab271183 at 1/2000 (0.297 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining in human lung carcinoma. The section was incubated with ab271183 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody [EPR24770-16] (ab271183)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HepG2 cells labelling TGN46 with ab271183 at 1/50 (11.88 μg/mL) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 μg/mL) dilution (Green). Confocal image showing co-locolization staining with golgi marker in HepG2 cells is observed. Anti-GM130 antibody - BSA and Azide free ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker was used to counterstain tubulin at 1/200 (5 μg/mL) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Negative Control 1: Secondary antibody only control: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.
Negative Control 2: Anti-GM130 antibody - BSA and Azide free ab169276 Anti-GM130 mouse polyclonal antibody - cis-Golgi Marker 1/200 (5 μg/ml) and Secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/mL) dilution.
Flow Cytometry (Intracellular) - Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (ab271183)
TGN46 Flow Cytometry (Intracellular) staining using rabbit Anti-TGN46 antibody
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HepG2 (human hepatocellular carcinoma epithelial cell) cells labelling TGN46 with ab271183 at 1/50 dilution (1 μg)/ (Red), compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730)/ (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-TGN46 antibody [EPR24770-16] - Golgi Marker (ab271183)
TGN46 Flow Cytometry (Intracellular) staining using rabbit Anti-TGN46 antibody
Flow cytometry overlay histogram showing wild-type A549 (green line) and TGOLN2 knockout A549 stained with ab271183 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilised with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab271183) (1x 106 in 100μl at 1.0 μg/ml (1/1980)) for 30min at 22°C.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min at 22°C

Isotype control antibody Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control was used at the same concentration and conditions as the primary antibody (wild-type A549 - black line, TGOLN2 knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

This antibody gave a positive signal in A549 Fixed with 4% formaldehyde (10 min) / permeabilised with 0.1% PBS-Triton X-100 for 15 min under the same conditions.