Anti-TGN46 antibody - Golgi Marker

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody - Golgi Marker (AB50595)

ab50595 staining TGN46 in HepG2 cells (top panel - positive control) and HeLa cells (bottom panel - negative control, low TGN46 expression). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab50595 at 1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor^® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor^® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• ICC/IF

AbReview18045****

Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody - Golgi Marker (AB50595)

ab50595 staining TGN46 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde in PBS for 10 minutes, permeabilized with 0.1% Triton X100 and blocked with 2% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA + 0.1% Triton X100) for 2 hours at 25°C. An Alexa Fluor^® 594-conjugated Goat polyclonal to mouse IgG (dilution 1/1500) was used as secondary antibody.

This image is courtesy of an anonymous Abreview

• ICC/IF

AbReview45993****

Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody - Golgi Marker (AB50595)

Immunocytochemistry/ Immunofluorescence analysis of Panc-1 cells labeling TGN46 with ab50595 at 1/100 dilution. Cells were fixed with formaldehyde followed by blocking in 1% Donkey serum in PBST for 1 hour at 22°C. A donkey-anti-Rb Alexa Fluor^® 594 was used as the secondary antibody at 1/200 dilution. DAPI nulcear counter stain.

This image is courtesy of an abreview submitted by Dr Armen Petrosyan, Univ of Nebraska.

• ICC

Lab

Immunocytochemistry - Anti-TGN46 antibody - Golgi Marker (AB50595)

ab50595 staining TGN46 in wild-type A549 cells (top panel) and TGOLN2 knockout A549 cells (bottom panel) (ab269510). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab50595 at 0.1μg/ml concentration and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor^® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor^® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody - Golgi Marker (AB50595)

Human A549 cells were fixed and permeabilized with methanol followed by acetone fixation. Fixed cells were stained with 2.5 µg/ml ab50595. The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.

• ICC/IF

AbReview12335****

Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody - Golgi Marker (AB50595)

ab50595 staining human osteosarcoma (143b) cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor® 594 conjugated donkey polyclonal to rabbit diluted 1/500.

This image is courtesy of Alessandro Marcello, Int Ctr Genet Engn & Biotechnol, Italy