Anti-TGN46 antibody (ab50595) is a rabbit polyclonal antibody that is used to detect TGN46 in ICC/IF. Suitable for Human samples.
- Over 70 publications
- Trusted since 2007
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- Nature communications 14:84402023NDP52 mediates an antiviral response to hepatitis B virus infection through Rab9-dependent lysosomal degradation pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShuzhi Cui,Tian Xia,Jianjin Zhao,Xiaoyu Ren,Tingtao Wu,Mireille Kameni,Xiaoju Guo,Li He,Jingao Guo,Aléria Duperray-Susini,Florence Levillayer,Jean-Marc Collard,Jin Zhong,Lifeng Pan,Frédéric Tangy,Pierre-Olivier Vidalain,Dongming Zhou,Yaming Jiu,Mathias Faure,Yu WeiPubMed 38114531
- PLoS pathogens 19:e10116482023Noncanonical Rab9a action supports retromer-mediated endosomal exit of human papillomavirus during virus entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJeongjoon Choi,Daniel DiMaioPubMed 37703297
- Communications biology 6:7022023Membrane recruitment of the polarity protein Scribble by the cell adhesion receptor TMIGD1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEva-Maria Thüring,Christian Hartmann,Janesha C Maddumage,Airah Javorsky,Birgitta E Michels,Volker Gerke,Lawrence Banks,Patrick O Humbert,Marc Kvansakul,Klaus EbnetPubMed 37430142
- Nature communications 14:9482023Identification of global inhibitors of cellular glycosylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel Madriz Sørensen,Christian Büll,Thomas D Madsen,Erandi Lira-Navarrete,Thomas Mandel Clausen,Alex E Clark,Aaron F Garretson,Richard Karlsson,Johan F A Pijnenborg,Xin Yin,Rebecca L Miller,Sumit K Chanda,Thomas J Boltje,Katrine T Schjoldager,Sergey Y Vakhrushev,Adnan Halim,Jeffrey D Esko,Aaron F Carlin,Ramon Hurtado-Guerrero,Roberto Weigert,Henrik Clausen,Yoshiki NarimatsuPubMed 36804936
- Science advances 9:eadc98302023HPV is a cargo for the COPI sorting complex during virus entry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMara C Harwood,Tai-Ting Woo,Yuka Takeo,Daniel DiMaio,Billy TsaiPubMed 36662862
- EMBO molecular medicine 15:e164782023A progeroid syndrome caused by a deep intronic variant in TAPT1 is revealed by RNA/SI-NET sequencing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNasrinsadat Nabavizadeh,Annkatrin Bressin,Mohammad Shboul,Ricardo Moreno Traspas,Poh Hui Chia,Carine Bonnard,Emmanuelle Szenker-Ravi,Burak Sarıbaş,Emmanuel Beillard,Umut Altunoglu,Zohreh Hojati,Scott Drutman,Susanne Freier,Mohammad El-Khateeb,Rajaa Fathallah,Jean-Laurent Casanova,Wesam Soror,Alaa Arafat,Nathalie Escande-Beillard,Andreas Mayer,Bruno ReversadePubMed 36652330
- bioRxiv : the preprint server for biology :2022Tetherin antagonism by SARS-CoV-2 enhances virus release: multiple mechanisms including ORF3a-mediated defective retrograde traffic.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHazel Stewart,Roberta Palmulli,Kristoffer H Johansen,Naomi McGovern,Ola M Shehata,George W Carnell,Hannah K Jackson,Jin S Lee,Jonathan C Brown,Thomas Burgoyne,Jonathan L Heeney,Klaus Okkenhaug,Andrew E Firth,Andrew A Peden,James R EdgarPubMed 33442692
- eLife 11:2022GJA1 depletion causes ciliary defects by affecting Rab11 trafficking to the ciliary base.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDong Gil Jang,Keun Yeong Kwon,Yeong Cheon Kweon,Byung-Gyu Kim,Kyungjae Myung,Hyun-Shik Lee,Chan Young Park,Taejoon Kwon,Tae Joo ParkPubMed 36004726
- Pathogens (Basel, Switzerland) 11:2022Infection of Human Macrophages Retains Golgi Structure but Reduces O-Glycans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanlin Fu,Vinitha Macwan,Rebecca Emily-Sue Heineman,Mauricio R Terebiznik,Rene E HarrisonPubMed 36015029
- STAR protocols 3:1015492022Immunofluorescence staining of phosphoinositides in primary mouse hippocampal neurons in dissociated culture.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenzhen Guo,Chunfang Tong,Yanrui Yang,Jia-Jia LiuPubMed 35842867
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TGOLN2 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O43493
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Human | Tested |
| Rat | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5.00000-10.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
May be involved in regulating membrane traffic to and from trans-Golgi network.
Alternative names
TGN46, TGN51, TGOLN2, Trans-Golgi network integral membrane protein 2, Trans-Golgi network glycoprotein 46, Trans-Golgi network glycoprotein 48, Trans-Golgi network glycoprotein 51, Trans-Golgi network protein 2, TGN38 homolog, hTGN46, hTGN48, hTGN51
Recommended products
Anti-TGN46 antibody (ab50595) is a rabbit polyclonal antibody that is used to detect TGN46 in ICC/IF. Suitable for Human samples.
- Over 70 publications
- Trusted since 2007
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TGOLN2 aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O43493
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-TGN46 antibody (ab50595) is a rabbit polyclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF), in Human samples.
Recommended positive controls
ICC/IF: Hep G2 and A549 cells.
Trusted by the scientific community
Anti-TGN46 (ab50595) was first used in a scientific publication in 2007 and has been cited over 70 times in peer-reviewed journals.
Reviewed by scientists
Anti-TGN46 (ab50595) has over 10 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TGN46 also known as trans-Golgi network protein 46 is an important membrane protein associated with the trans-Golgi network. The protein has a mass of approximately 46 kDa and plays a role in the sorting and trafficking of proteins to their proper destinations within the cell. TGN46 is widely expressed in various cell types and is commonly used as a Golgi marker in cell biology studies helping to identify Golgi apparatus in cells.
- Biological function summary
The protein TGN46 functions in the sorting and transport mechanisms within the cell. It does not function as a part of a large macromolecular complex but it interacts with several other proteins to facilitate the movement of proteins from the Golgi to other sites within the cell. The protein also associates with trafficking components to ensure the correct delivery of proteins to the plasma membrane or lysosomes.
- Pathways
The role of TGN46 involves participation in intracellular transport and protein trafficking pathways. It interacts with clathrin-coated vesicles and works closely with proteins such as gamma-adaptin and epsin which are important for vesicle formation and transport. These interactions highlight its involvement in protein secretion and membrane transport pathways which are essential for maintaining cellular homeostasis.
- Associated diseases and disorders
TGN46 appears to have connections to neurodegenerative diseases and certain forms of cancer although the mechanisms are not fully understood. Alterations in TGN46 have been observed in Alzheimer's disease where it may intersect with proteins like amyloid precursor protein (APP) in protein misfolding and aggregation pathways. Additionally changes in TGN46 expression levels have been implicated in prostate cancer potentially due to its role in protein sorting and dysregulation of cellular trafficking processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)
ab50595 staining TGN46 in HepG2 cells (top panel - positive control) and HeLa cells (bottom panel - negative control, low TGN46 expression). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab50595 at 1μg/ml concentration and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
This image is courtesy of Alessandro Marcello, Int Ctr Genet Engn & Biotechnol, ItalyImmunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody - Golgi Marker (ab50595)
TGN46 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-TGN46 antibody
ab50595 staining human osteosarcoma (143b) cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 1% BSA for 30 minutes at 37°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37°C. The secondary antibody was Alexa Fluor® 594 conjugated donkey polyclonal to rabbit diluted 1/500.
This image is courtesy of a customer review submitted by Dr Armen Petrosyan, Univ of Nebraska.Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody (ab50595)
Immunocytochemistry/ Immunofluorescence analysis of Panc-1 cells labeling TGN46 with ab50595 at 1/100 dilution. Cells were fixed with formaldehyde followed by blocking in 1% Donkey serum in PBST for 1 hour at 22°C. A donkey-anti-Rb Alexa Fluor® 594 was used as the secondary antibody at 1/200 dilution. DAPI nulcear counter stain.
Immunocytochemistry - Anti-TGN46 antibody (ab50595)
ab50595 staining TGN46 in wild-type A549 cells (top panel) and TGOLN2 knockout A549 cells (bottom panel) (Human TGOLN2 (TGN46) knockout A549 cell line ab269510). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab50595 at 0.1μg/ml concentration and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody - Golgi Marker (ab50595)
TGN46 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-TGN46 antibody
ab50595 staining TGN46 in human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde in PBS for 10 minutes, permeabilized with 0.1% Triton X100 and blocked with 2% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 2% BSA + 0.1% Triton X100) for 2 hours at 25°C. An Alexa Fluor® 594-conjugated Goat polyclonal to mouse IgG (dilution 1/1500) was used as secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-TGN46 antibody - Golgi Marker (ab50595)
TGN46 Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-TGN46 antibody
Human A549 cells were fixed and permeabilized with methanol followed by acetone fixation. Fixed cells were stained with 2.5 µg/ml ab50595. The antibody was developed with Goat Anti-Rabbit IgG, FITC conjugate.
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