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AB325658

Anti-TH antibody [LNC1] - Chicken IgY (Chimeric)

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Chicken Monoclonal Tyrosine Hydroxylase antibody. Suitable for IHC-P, WB and reacts with Rat samples.

View Alternative Names

Tyrosine 3-monooxygenase, Tyrosine 3-hydroxylase, TH, Th

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TH antibody [LNC1] - Chicken IgY (Chimeric) (AB325658)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TH antibody [LNC1] - Chicken IgY (Chimeric) (AB325658)

Representative section of formalin fixed, paraffin-embedded rat brain mid-brain region showing cells stained by ab325658. The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the dopamine neurons in the mid-brain. Magnification 400X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TH antibody [LNC1] - Chicken IgY (Chimeric) (AB325658)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TH antibody [LNC1] - Chicken IgY (Chimeric) (AB325658)

Representative section of formalin fixed, paraffin-embedded rat brain mid-brain region showing cells stained by ab325658 (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the dopamine neurons in the mid-brain. Magnification 100X.

Western blot - Anti-TH antibody [LNC1] - Chicken IgY (Chimeric) (AB325658)
  • WB

Supplier Data

Western blot - Anti-TH antibody [LNC1] - Chicken IgY (Chimeric) (AB325658)

Western blotting of rat brain homogenate with an anti-Tyrosine Hydroxylase mouse mAb and ab325658. The mouse mAb and ab325658 were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. ab325658 recognizes endogenous TH with similar sensitivity as the anti-TH mouse mAb in the rat brain homogenate at 59-63 kDa.

All lanes:

Western blot - Anti-TH antibody [LNC1] - Chicken IgY (Chimeric) (ab325658) at 1/1000 dilution

All lanes:

rat brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 59-63 kDa

false

Key facts

Host species

Chicken

Clonality

Monoclonal

Clone number

LNC1

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recognizes an epitope outside of the regulatory N-terminus. Recognizes a protein of approximately 59-61 kDa by Western blot. Does not react with the following on Western Blots: purified dopamine-beta-hydroxylase, phenylalanine hydroxylase, trytophan hydroxylase, dehydropteridine reductase, sepiapterin reductase or phenethanolamine-N-methyl transferase (PNMT). Identifies a single ~60 kDa band on Western Blots of HeLa cells transfected to express human tyrosine hydroxylase.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone LNC1 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone LNC1 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of L-tyrosine to L-dihydroxyphenylalanine (L-Dopa), the rate-limiting step in the biosynthesis of cathecolamines, dopamine, noradrenaline, and adrenaline. Uses tetrahydrobiopterin and molecular oxygen to convert tyrosine to L-Dopa (By similarity). In addition to tyrosine, is able to catalyze the hydroxylation of phenylalanine and tryptophan but with lower specificity (PubMed : 10933781, PubMed : 11922614). Positively regulates the regression of retinal hyaloid vessels during postnatal development (By similarity).
See full target information TH
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Product promise

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