AB249604

Anti-TH1L antibody [EPR11594] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TH1L antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

NELFD, TH1, TH1L, HSPC130, NELFCD, Negative elongation factor C/D, NELF-C/D, TH1-like protein

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-TH1L antibody [EPR11594] - BSA and Azide free (AB249604)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TH1L antibody [EPR11594] - BSA and Azide free (AB249604)

This data was developed using ab170934, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of HeLa cells labeling TH1L with ab170934 at 1/50 dilution.

Supplier Data

Western blot - Anti-TH1L antibody [EPR11594] - BSA and Azide free (AB249604)

This data was developed using ab170934, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-TH1L antibody [EPR11594] (<a href='/en-us/products/primary-antibodies/th1l-antibody-epr11594-ab170934'>ab170934</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysates at 10 µg

Lane 2:

HeLa cell lysates at 10 µg

Lane 3:

MCF-7 cell lysates at 10 µg

Lane 4:

HCT-116 cell lysates at 10 µg

Predicted band size: 66 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11594

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab249604 is the carrier-free version of ab170934.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TH1L also known as Mediator Complex Subunit 22 (MED22) is a mechanically important component of the mediator complex a multi-protein structure that acts as a bridge between gene-specific transcription factors and the RNA polymerase II enzyme facilitating the transcription of messenger RNA. TH1L has a molecular weight of approximately 36 kDa. It is predominantly expressed in the nucleus of various cell types often in actively dividing or metabolically active tissues.

Biological function summary

The role of TH1L extends beyond just a mechanical scaffold. As part of the mediator complex TH1L plays an important role in regulating gene expression by interacting with transcriptional activators and repressors. It integrates signals from transcription factors and conveys them to the transcriptional machinery thereby influencing which genes are turned on or off. TH1L is instrumental in the coordinated control of gene expression impacting diverse biological processes such as cell growth differentiation and response to external stimuli.

Pathways

TH1L's involvement is significant in the Notch signaling pathway and the Wnt signaling pathway both important for cell fate determination and proliferation. In the Notch pathway TH1L helps regulate the transcription of target genes by modulating the activity of the Notch intracellular domain. In the Wnt signaling TH1L assists in the transcriptional regulation by interacting with transcription factors like TCF/LEF that respond to Wnt signals. These pathways form a complex network of protein interactions with TH1L working in conjunction with other proteins like SMAD and β-catenin to fine-tune gene expression outcomes.

Research indicates TH1L involvement in cancer and autoimmune disorders. In certain cancers such as breast and prostate cancer dysregulation of TH1L leads to aberrant gene expression profiles that promote tumorigenesis. The abnormal function of TH1L through its interactions with oncogenic proteins like MYC can accelerate cell proliferation. In autoimmune disorders misregulation of TH1L may alter immune cell activity linking it to proteins involved in the immune response like NF-κB exacerbating the inappropriate immune activation that characterizes these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II (PubMed : 12612062). The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex (PubMed : 10199401).. (Microbial infection) The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II.

See full target information NELFCD

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Bio-protocol 15:e5206 PubMed39959296

2025

Cell-Sonar, an Easy and Low-cost Method to Track a Target Protein by Expression Changes of Specific Protein Markers.

Applications

Unspecified application

Species

Unspecified reactive species

Sabrina Brockmöller,Lara Maria Molitor,Franz Worek,Simone Rothmiller

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TH1L antibody [EPR11594] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-TH1L antibody [EPR11594] - BSA and Azide free (AB249604)

Western blot - Anti-TH1L antibody [EPR11594] - BSA and Azide free (AB249604)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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