AB211143

Anti-THBS4 antibody - N-terminal

Name: Anti-THBS4 antibody - N-terminal (ab211143) | Abcam
Brand: Abcam Limited
SKU: AB211143
Rating: 5 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal TSP4B antibody. N-terminal. Suitable for WB, IHC - Wmt and reacts with Zebrafish samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Zebrafish thbs4b aa 1-350.

View Alternative Names

thbs4, tsp4, thbs4b, Thrombospondin-4-B, Thbs4-B

3 Images

IHC - Wholemount - Anti-THBS4 antibody - N-terminal (AB211143)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-THBS4 antibody - N-terminal (AB211143)

Wholemount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryo labeling THBS4 with ab211143 at 1/100 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-THBS4 antibody - N-terminal (AB211143)

Wholemount immunohistochemical analysis of 2 days-post-fertilization zebrafish embryo labeling THBS4 with ab211143 at 1/100 dilution.

Supplier Data

Western blot - Anti-THBS4 antibody - N-terminal (AB211143)

7.5% SDS-PAGE

All lanes:

Western blot - Anti-THBS4 antibody - N-terminal (ab211143) at 1/1000 dilution

All lanes:

Zebrafish muscle whole cell lysate at 30 µg

Predicted band size: 106 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

IHC - Wmt, WB

applications

Immunogen

Recombinant Fragment Protein within Zebrafish thbs4b aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q8JGW0

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THBS4 also known as Thrombospondin-4 is a glycoprotein with a mass of approximately 105 kDa. It is part of the thrombospondin family which is known for binding to various cell surface receptors and components of the extracellular matrix. THBS4 is expressed in many tissues including the brain heart and skeletal muscle. Its structure allows it to interact with other proteins and play a role in cell-matrix communication.

Biological function summary

THBS4 interacts with molecules in the extracellular matrix to regulate cellular functions such as adhesion migration and proliferation. It often operates within a complex network of proteins modulating cell interactions and tissue remodeling. This protein contributes to synaptic functions in the nervous system and soft tissue repair. The presence of THBS4 in different tissue types suggests it has diverse roles across the body's systems.

Pathways

This protein is a component of the signaling pathways related to inflammatory and repair processes. THBS4 participates in the TGF-beta (Transforming Growth Factor-beta) signaling pathway which involves other proteins like TGF-beta itself and integrins. It influences wound healing and fibrosis through its effects on cellular behavior and signaling cascades.

Alterations in THBS4 levels can associate with conditions such as myocardial infarction and osteoarthritis. Abnormal expression or function of THBS4 can disrupt normal tissue repair and inflammation control contributing to disease progression. The protein's interaction with integrins and collagen is significant in understanding its role in these conditions highlighting its potential as a therapeutic target for modulating tissue repair and disease processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions and may be involved in various processes including cellular proliferation, migration, adhesion and attachment. May play a role in ER stress response (By similarity).

See full target information thbs4b

style

left-column

Publications (4)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 7: PubMed35579956

2022

A pathogenic mechanism associated with myopathies and structural birth defects involves TPM2-directed myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer McAdow,Shuo Yang,Tiffany Ou,Gary Huang,Matthew B Dobbs,Christina A Gurnett,Michael J Greenberg,Aaron N Johnson

Developmental dynamics : an official publication of the American Association of Anatomists 252:104-123 PubMed35708710

2022

Zebrafish Erc1b mediates motor innervation and organization of craniofacial muscles in control of jaw movement.

Applications

Unspecified application

Species

Unspecified reactive species

Lauryn N Luderman,Mackenzie T Michaels,Daniel S Levic,Ela W Knapik

Nature medicine 26:98-109 PubMed31932796

2020

Phenome-based approach identifies RIC1-linked Mendelian syndrome through zebrafish models, biobank associations and clinical studies.

Applications

Unspecified application

Species

Unspecified reactive species

Gokhan Unlu,Xinzi Qi,Eric R Gamazon,David B Melville,Nisha Patel,Amy R Rushing,Mais Hashem,Abdullah Al-Faifi,Rui Chen,Bingshan Li,Nancy J Cox,Fowzan S Alkuraya,Ela W Knapik

FASEB journal : official publication of the Federa 33:9116-9130 PubMed31100023

2019

Scleraxis genes are required for normal musculoskeletal development and for rib growth and mineralization in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Erika Kague,Simon M Hughes,Elizabeth A Lawrence,Stephen Cross,Elizabeth Martin-Silverstone,Chrissy L Hammond,Yaniv Hinits

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-THBS4 antibody - N-terminal

IHC - Wholemount - Anti-THBS4 antibody - N-terminal (AB211143)

IHC - Wholemount - Anti-THBS4 antibody - N-terminal (AB211143)

Western blot - Anti-THBS4 antibody - N-terminal (AB211143)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise