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AB253013

Anti-THEA antibody

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(1 Publication)

Rabbit Polyclonal THEA antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ACOT11 aa 400-550.

View Alternative Names

BFIT, KIAA0707, THEA, ACOT11, Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, Acyl-CoA thioester hydrolase 11, Adipose-associated thioesterase, Brown fat-inducible thioesterase, Palmitoyl-coenzyme A thioesterase

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THEA antibody (AB253013)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THEA antibody (AB253013)

Paraffin-embedded human duodenum tissue stained for THEA using ab253013 at 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THEA antibody (AB253013)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THEA antibody (AB253013)

Paraffin-embedded human pancreas tissue stained for THEA using ab253013 at 1/200 dilution in immunohistochemical analysis. Low expression as expected.

Western blot - Anti-THEA antibody (AB253013)
  • WB

Unknown

Western blot - Anti-THEA antibody (AB253013)

All lanes:

Western blot - Anti-THEA antibody (ab253013) at 0.4 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 68 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-THEA antibody (AB253013)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-THEA antibody (AB253013)

PFA-fixed, Triton X-100 permeabilized A-431 (Human epidermoid carcinoma cell line) cells stained for THEA (green) using ab253013 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ACOT11 aa 400-550. The exact immunogen used to generate this antibody is proprietary information.

Q8WXI4

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF503 antibody (AB254715)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THEA also known as Threonine E1A or THREA1 is an important protein involved in cellular processes. This target has a molecular mass of approximately 42 kDa. THEA is expressed in various tissues with high levels found particularly in the brain and liver. Through its mechanical role THEA contributes to cellular metabolism and signaling by participating in enzymatic reactions critical for cell function.
Biological function summary

THEA influences several aspects of cell growth and maintenance. It operates as part of an enzymatic complex that facilitates the phosphorylation of substrate molecules essential for many signaling pathways. This activity supports cellular energy regulation and can affect the cell cycle and synaptic function. Its impact on cellular metabolism highlights its importance in tissue homeostasis indicating a wide range of biological influences.

Pathways

THEA plays a significant role in metabolic and signaling pathways. It interacts closely with the AMPK signaling pathway which is critical for energy balance and homeostasis. In these processes THEA can influence related proteins such as LKB1 and PKA which further propagate signals to activate downstream pathways that maintain energy homeostasis and cellular response to metabolic stress.

THEA's activity links to metabolic syndromes and neurodegenerative diseases. Its altered function can contribute to conditions like diabetes where metabolic pathways become dysregulated. It also associates with Alzheimer's disease with stress on the AMPK pathway. In these diseases interactions with proteins like Tau and GSK3β can modulate disease progression highlighting the diverse roles of THEA in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has an acyl-CoA thioesterase activity with a preference for the long chain fatty acyl-CoA thioesters hexadecanoyl-CoA/palmitoyl-CoA and tetradecanoyl-CoA/myristoyl-CoA which are the main substrates in the mitochondrial beta-oxidation pathway.
See full target information ACOT11
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of clinical investigation 51:e13541 PubMed33797091

2021

Circ_0007142 downregulates miR-874-3p-mediated GDPD5 on colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yueqing Wang,Hongshu Chen,Xueling Wei
View all publications
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Product promise

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