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Rabbit Recombinant Monoclonal THEA antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.


Images

Western blot - Anti-THEA antibody [EPR18792] (AB180745), expandable thumbnail
  • Western blot - Anti-THEA antibody [EPR18792] (AB180745), expandable thumbnail
  • Western blot - Anti-THEA antibody [EPR18792] (AB180745), expandable thumbnail
  • Western blot - Anti-THEA antibody [EPR18792] (AB180745), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Has an acyl-CoA thioesterase activity with a preference for the long chain fatty acyl-CoA thioesters hexadecanoyl-CoA/palmitoyl-CoA and tetradecanoyl-CoA/myristoyl-CoA which are the main substrates in the mitochondrial beta-oxidation pathway.

Alternative names

BFIT, KIAA0707, THEA, ACOT11, Acyl-coenzyme A thioesterase 11, Acyl-CoA thioesterase 11, Acyl-CoA thioester hydrolase 11, Adipose-associated thioesterase, Brown fat-inducible thioesterase, Palmitoyl-coenzyme A thioesterase

Recommended products

Rabbit Recombinant Monoclonal THEA antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR18792
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

THEA also known as Threonine E1A or THREA1 is an important protein involved in cellular processes. This target has a molecular mass of approximately 42 kDa. THEA is expressed in various tissues with high levels found particularly in the brain and liver. Through its mechanical role THEA contributes to cellular metabolism and signaling by participating in enzymatic reactions critical for cell function.

Biological function summary

THEA influences several aspects of cell growth and maintenance. It operates as part of an enzymatic complex that facilitates the phosphorylation of substrate molecules essential for many signaling pathways. This activity supports cellular energy regulation and can affect the cell cycle and synaptic function. Its impact on cellular metabolism highlights its importance in tissue homeostasis indicating a wide range of biological influences.

Pathways

THEA plays a significant role in metabolic and signaling pathways. It interacts closely with the AMPK signaling pathway which is critical for energy balance and homeostasis. In these processes THEA can influence related proteins such as LKB1 and PKA which further propagate signals to activate downstream pathways that maintain energy homeostasis and cellular response to metabolic stress.

Associated diseases and disorders

THEA's activity links to metabolic syndromes and neurodegenerative diseases. Its altered function can contribute to conditions like diabetes where metabolic pathways become dysregulated. It also associates with Alzheimer's disease with stress on the AMPK pathway. In these diseases interactions with proteins like Tau and GSK3β can modulate disease progression highlighting the diverse roles of THEA in pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-THEA antibody [EPR18792] (ab180745), expandable thumbnail

    Western blot - Anti-THEA antibody [EPR18792] (ab180745)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2/3: 15 seconds.

    All lanes: Western blot - Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution

    Lane 1: Human fetal liver tissue lysate at 10 µg

    Lane 2: Human fetal heart tissue lysate at 10 µg

    Lane 3: Human fetal kidney tissue lysate at 10 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

  • Western blot - Anti-THEA antibody [EPR18792] (ab180745), expandable thumbnail

    Western blot - Anti-THEA antibody [EPR18792] (ab180745)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 3 minutes; Lane 2/3: 15 seconds.

    This antibody can recognize 2 isoforms in human, the predicted MW is 68 & 67 KDa.

    All lanes: Western blot - Anti-THEA antibody [EPR18792] (ab180745) at 1/2000 dilution

    Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Lane 2: LNCaP (human prostate cancer cell line) whole cell lysate at 20 µg

    Lane 3: HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa

    Observed band size: 67 kDa, 68 kDa

  • Western blot - Anti-THEA antibody [EPR18792] (ab180745), expandable thumbnail

    Western blot - Anti-THEA antibody [EPR18792] (ab180745)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    The expression profile observed is consistent with what has been described in the literature (PMID: 22427358 and PMID:11696000).

    All lanes: Western blot - Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution

    Lane 1: Mouse brown adipose lysate at 20 µg

    Lane 2: Mouse liver lysate at 20 µg

    Lane 3: Mouse kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 68 kDa

    Observed band size: 68 kDa

  • Western blot - Anti-THEA antibody [EPR18792] (ab180745), expandable thumbnail

    Western blot - Anti-THEA antibody [EPR18792] (ab180745)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/2: 1 minute; Lane 3: 10 seconds; Lane 4: 15 seconds.

    All lanes: Western blot - Anti-THEA antibody [EPR18792] (ab180745) at 1/1000 dilution

    Lane 1: Mouse heart lysate at 10 µg

    Lane 2: Mouse spleen lysate at 10 µg

    Lane 3: Mouse kidney lysate at 10 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 41 kDa, 47 kDa, 68 kDa

    Observed band size: 68 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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