Anti-THEM2 antibody

• WB

Lab

Western blot - Anti-THEM2 antibody (AB228011)

Western blot : Anti-ACOT13 antibody (ab228011) staining at 4 ug/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab228011 was shown to bind specifically to ACOT13. A band was observed at 16 kDa in wild-type K562 cell lysates with no signal observed at this size in ACOT13 knockout cell line. To generate this image, wild-type and ACOT13 knockout K562 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-THEM2 antibody (ab228011) at 4 µg/mL

Lane 1:

Wild-type K562 cell lysate at 20 µg

Lane 2:

ACOT13 knockout K562 cell lysate at 20 µg

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

THP-1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

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• WB

Supplier Data

Western blot - Anti-THEM2 antibody (AB228011)

All lanes:

Western blot - Anti-THEM2 antibody (ab228011) at 2 µg/mL

Lane 1:

PANC-1 (human pancreatic epithelial cancinoma cell line) cell lysate

Lane 2:

Human brain tissue lysate

Predicted band size: 14 kDa

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