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AB106435

Anti-THEM4 antibody

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(4 Publications)

Rabbit Polyclonal THEM4 antibody. Suitable for WB, ICC/IF, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human THEM4.

View Alternative Names

CTMP, THEM4, Acyl-coenzyme A thioesterase THEM4, Acyl-CoA thioesterase THEM4, Carboxyl-terminal modulator protein, Thioesterase superfamily member 4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-THEM4 antibody (AB106435)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-THEM4 antibody (AB106435)

Immunofluorescence of THEM4 in Human liver tissue with ab106435 at 20 ug/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THEM4 antibody (AB106435)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THEM4 antibody (AB106435)

ab106435 at 2.5μg staining THEM4 in paraffin embedded Human liver tissue by immunohistochemistry.

Western blot - Anti-THEM4 antibody (AB106435)
  • WB

Unknown

Western blot - Anti-THEM4 antibody (AB106435)

Lane 1:

Western blot - Anti-THEM4 antibody (ab106435) at 1 µg/mL

Lane 2:

Western blot - Anti-THEM4 antibody (ab106435) at 2 µg/mL

All lanes:

Human liver tissue lysate at 15 µg

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human THEM4. The exact immunogen used to generate this antibody is proprietary information.

Q5T1C6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "2.5 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THEM4 also known as CTMP (carboxyl-terminal modulator protein) has a mass of approximately 25 kDa. This protein functions by interacting with the protein kinase B (PKB/Akt) signaling pathway modulating its activity. It locates in the cytoplasm and mitochondria and expresses in various tissues with higher levels found in the heart skeletal muscle and liver. THEM4 plays a role in regulating cellular processes related to metabolism and apoptosis.
Biological function summary

THEM4 affects several cellular mechanisms. It associates with mitochondrial homeostasis and energy metabolism. THEM4 acts as an inhibitory regulator of Akt which is critical for cell survival and growth processes. It interacts with other proteins to influence energy homeostasis contributing to lipid metabolism regulation. As part of protein complexes THEM4 may function in concert with other regulatory proteins to perform its roles efficiently.

Pathways

THEM4 significantly impacts the PI3K/Akt signaling pathway facilitating communication between the cell membrane and intracellular targets affecting cell growth and survival. It also links to the mTOR pathway which THEM4 influences through its interaction with Akt. THEM4 modulates these critical pathways by interacting with proteins such as PTEN which serves as a phosphatase and GSK-3β a kinase involved in cellular glycogen metabolism.

THEM4 connects to cancer and cardiovascular diseases through its regulatory effects on Akt activity. In cancer altered THEM4 function can lead to excessive cell survival and growth contributing to tumorigenesis. Its modulation of Akt implicates THEM4 in cardiovascular disease where Akt is critical for maintaining cardiac function. THEM4's interaction with proteins like Bcl-2 in apoptosis and p53 in DNA repair and cell cycle control further connects it to these disorders offering avenues for targeted therapies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has acyl-CoA thioesterase activity towards medium and long-chain (C14 to C18) fatty acyl-CoA substrates, and probably plays a role in mitochondrial fatty acid metabolism. Plays a role in the apoptotic process, possibly via its regulation of AKT1 activity. According to PubMed : 11598301, inhibits AKT1 phosphorylation and activity. According to PubMed : 17615157, enhances AKT1 activity by favoring its phosphorylation and translocation to plasma membrane.
See full target information THEM4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 240:e70007 PubMed39888066

2025

Carboxyl Terminal Modulator Protein Induces Cell Senescence and Is Upregulated With Aging by Zic2 in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Weiran Shan,Jun Li,Zachary Philpot,Zhiyi Zuo

Frontiers in oncology 11:672684 PubMed34249713

2021

Exosomal miR-183-5p Shuttled by M2 Polarized Tumor-Associated Macrophage Promotes the Development of Colon Cancer Targeting THEM4 Mediated PI3K/AKT and NF-κB Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Shangxin Zhang,Deguan Li,Min Zhao,Fei Yang,Changye Sang,Changhong Yan,Zhenjun Wang,Yongxiang Li

American journal of cancer research 11:2568-2589 PubMed34249416

2021

ELOVL2: a novel tumor suppressor attenuating tamoxifen resistance in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Dawoon Jeong,Juyeon Ham,Hyeon Woo Kim,Heejoo Kim,Hwee Won Ji,Sung Hwan Yun,Jae Eun Park,Keun Seok Lee,Heein Jo,Jai Hong Han,So-Youn Jung,Seeyoun Lee,Eun Sook Lee,Han-Sung Kang,Sun Jung Kim

Molecular neurobiology 55:6145-6154 PubMed29250714

2017

Age-Related Upregulation of Carboxyl Terminal Modulator Protein Contributes to the Decreased Brain Ischemic Tolerance in Older Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Li,Weiran Shan,Zhiyi Zuo
View all publications
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