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AB248330

Anti-Themis antibody [EPR7354] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Themis antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C6orf190, C6orf207, THEMIS, Protein THEMIS, Thymocyte-expressed molecule involved in selection

1 Images
Western blot - Anti-Themis antibody [EPR7354] - BSA and Azide free (AB248330)
  • WB

Unknown

Western blot - Anti-Themis antibody [EPR7354] - BSA and Azide free (AB248330)

This data was developed using ab129174, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Themis antibody [EPR7354] - BSA and Azide free (ab248330) at 1/10000 dilution

Lane 1:

Human thymus lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7354

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab248330 is the carrier-free version of ab129174.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Themis also known as thymocyte-expressed molecule involved in selection is a protein involved in T-cell development. Themis has a mass of approximately 64 kDa. Researchers have found it expressed specifically in thymocytes a type of immune cell found in the thymus. Mechanically Themis helps in signal transduction processes necessary for the proper development and selection of T-cells which are important components of the adaptive immune system. Themis functions by binding to and modulating various signaling molecules within the immune cells.
Biological function summary

Themis plays a significant role in T-cell receptor (TCR) signaling which is essential for the positive and negative selection of thymocytes. Themis functions as part of a complex that cooperates with other proteins to fine-tune the thresholds for TCR signaling. Through its action it ensures the proper development of functional and self-tolerant T-cells helping to distinguish self from non-self in immune responses. As cells progress through the thymus Themis regulates the strength and duration of signaling ensuring the appropriate intensity for T-cell maturation.

Pathways

T-cell development heavily relies on Themis for its contribution to the TCR signaling pathway. Themis interacts in this pathway with important proteins such as LAT (Linker for Activation of T cells) and SLP-76 which are key components for propagating downstream signaling that leads to T-cell activation. Additionally Themis is involved with the Ras-MAPK signaling pathway which further contributes to cell proliferation and differentiation. Both pathways are critical for maintaining the balance in the immune response allowing for the selection of T-cells that can combat infections while avoiding autoimmunity.

Disruptions in Themis function can link to autoimmune diseases and severe combined immunodeficiency (SCID). Mutations or misregulation of Themis can cause improper T-cell selection leading to the survival of self-reactive T-cells or failure in T-cell development. For example defective Themis activity has connections to autoimmune disorders where the body attacks its own tissues. In such cases proteins like LAT and SLP-76 can also show altered activity which contributes to the development and progression of these immune-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
See full target information THEMIS
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell proliferation 56:e13400 PubMed36642844

2023

Non-SMC condensin I complex subunit H participates in anti-programmed cell death-1 resistance of clear cell renal cell carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi Chen,Weiqiang Ruan,Chunhao Guo,Ke Chen,Le Li,Jihua Tian,Zhiquan Hu,Dan Peng,Xing Zeng
View all publications
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Product promise

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