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AB154173

Anti-Thimet Oligopeptidase antibody [EPR7651]

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(2 Publications)

Rabbit Recombinant Monoclonal Thimet Oligopeptidase antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

Thimet oligopeptidase, Endopeptidase 24.15, MP78, THOP1

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Thimet Oligopeptidase antibody [EPR7651] (AB154173)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Thimet Oligopeptidase antibody [EPR7651] (AB154173)

Immunofluorescence analysis of MCF7 cells labeling Thimet Oligopeptidase with ab154173 at 1/100 dilution.

Western blot - Anti-Thimet Oligopeptidase antibody [EPR7651] (AB154173)
  • WB

Unknown

Western blot - Anti-Thimet Oligopeptidase antibody [EPR7651] (AB154173)

All lanes:

Western blot - Anti-Thimet Oligopeptidase antibody [EPR7651] (ab154173) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Lane 5:

THP1 cell lysate at 10 µg

Lane 6:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 79 kDa

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  • Carrier free

    Anti-Thimet Oligopeptidase antibody [EPR7651] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7651

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thimet Oligopeptidase also known as EC 3.4.24.15 or TOP functions as a zinc-dependent metallopeptidase. It breaks down peptides that are up to 20 amino acids long by cleaving peptide bonds. It has a molecular mass of approximately 78 kDa. This enzyme is expressed in various tissues prominently in the brain but also found in the kidneys lungs and testis reflecting its broad physiological roles. TOP primarily exists in the cytosol but can translocate to other cellular compartments under specific conditions.
Biological function summary

Thimet Oligopeptidase plays a significant role in modulating neuropeptide levels in the nervous system. It does not operate as part of a known complex but instead acts independently in intracellular peptide catabolism. This activity helps regulate processes such as pain modulation and neuroendocrine signaling by modifying peptide-based signaling molecules. Furthermore TOP participates in the degradation of antigenic peptides implicating a role in immune system regulation.

Pathways

Thimet Oligopeptidase participates in neuropeptide and peptide hormone metabolism where it helps maintain homeostasis by modulating bioactive peptide concentrations. Within these pathways TOP interacts with other proteins such as neprilysin and angiotensin-converting enzyme creating a network that helps regulate cardiovascular and neural functions. This interaction highlights the enzyme's versatile capability to influence multiple signaling cascades.

Thimet Oligopeptidase connects strongly to neurodegenerative diseases such as Alzheimer's and Parkinson's. Research indicates that abnormal activity of TOP contributes to the accumulation of toxic peptide fragments like amyloid-beta in Alzheimer’s disease. This connection brings it into association with proteins like amyloid precursor protein (APP) and tau which are key in such neurodegenerative conditions. The enzyme's regulation of peptidase activity in these contexts highlights its potential as a therapeutic target for disease intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation (PubMed : 17251185, PubMed : 7639763). Able to degrade the amyloid-beta precursor protein and generate amyloidogenic fragments (PubMed : 17251185, PubMed : 7639763). Also acts as a regulator of cannabinoid signaling pathway by mediating degradation of hemopressin, an antagonist peptide of the cannabinoid receptor CNR1 (By similarity).
See full target information Thimet oligopeptidase
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39145770

2024

RGS10 deficiency facilitates distant metastasis by inducing epithelial-mesenchymal transition in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Yi Jiang,Peng Qiu,Tie Ma,Yang Bai,Jiawen Bu,Yueting Hu,Ming Jin,Tong Zhu,Xi Gu

Biomedicines 10: PubMed35453612

2022

Enhanced Antitumor Efficacy of PhAc-ALGP-Dox, an Enzyme-Activated Doxorubicin Prodrug, in a Panel of THOP1-Expressing Patient-Derived Xenografts of Soft Tissue Sarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Britt Van Renterghem,Agnieszka Wozniak,Ludovica Tarantola,Andrea Casazza,Jasmien Wellens,Madita Nysen,Ulla Vanleeuw,Che-Jui Lee,Geert Reyns,Raf Sciot,Nele Kindt,Patrick Schöffski
View all publications
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Product promise

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