AB249073

Anti-Thimet Oligopeptidase antibody [EPR7651] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal Thimet Oligopeptidase antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Thimet oligopeptidase, Endopeptidase 24.15, MP78, THOP1

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Thimet Oligopeptidase antibody [EPR7651] - BSA and Azide free (AB249073)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Thimet Oligopeptidase antibody [EPR7651] - BSA and Azide free (AB249073)

This data was developed using ab154173, the same antibody clone in a different buffer formulation.Immunofluorescence analysis of MCF7 cells labeling Thimet Oligopeptidase with ab154173 at 1/100 dilution.

Unknown

Western blot - Anti-Thimet Oligopeptidase antibody [EPR7651] - BSA and Azide free (AB249073)

This data was developed using ab154173, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Thimet Oligopeptidase antibody [EPR7651] (<a href='/en-us/products/primary-antibodies/thimet-oligopeptidase-antibody-epr7651-ab154173'>ab154173</a>) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human fetal brain lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Lane 5:

THP1 cell lysate at 10 µg

Lane 6:

MCF7 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 79 kDa

false

Unconjugated

Anti-Thimet Oligopeptidase antibody [EPR7651]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7651

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab249073 is the carrier-free version of ab154173.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thimet Oligopeptidase also known as EC 3.4.24.15 or TOP functions as a zinc-dependent metallopeptidase. It breaks down peptides that are up to 20 amino acids long by cleaving peptide bonds. It has a molecular mass of approximately 78 kDa. This enzyme is expressed in various tissues prominently in the brain but also found in the kidneys lungs and testis reflecting its broad physiological roles. TOP primarily exists in the cytosol but can translocate to other cellular compartments under specific conditions.

Biological function summary

Thimet Oligopeptidase plays a significant role in modulating neuropeptide levels in the nervous system. It does not operate as part of a known complex but instead acts independently in intracellular peptide catabolism. This activity helps regulate processes such as pain modulation and neuroendocrine signaling by modifying peptide-based signaling molecules. Furthermore TOP participates in the degradation of antigenic peptides implicating a role in immune system regulation.

Pathways

Thimet Oligopeptidase participates in neuropeptide and peptide hormone metabolism where it helps maintain homeostasis by modulating bioactive peptide concentrations. Within these pathways TOP interacts with other proteins such as neprilysin and angiotensin-converting enzyme creating a network that helps regulate cardiovascular and neural functions. This interaction highlights the enzyme's versatile capability to influence multiple signaling cascades.

Thimet Oligopeptidase connects strongly to neurodegenerative diseases such as Alzheimer's and Parkinson's. Research indicates that abnormal activity of TOP contributes to the accumulation of toxic peptide fragments like amyloid-beta in Alzheimer’s disease. This connection brings it into association with proteins like amyloid precursor protein (APP) and tau which are key in such neurodegenerative conditions. The enzyme's regulation of peptidase activity in these contexts highlights its potential as a therapeutic target for disease intervention.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation (PubMed : 17251185, PubMed : 7639763). Able to degrade the amyloid-beta precursor protein and generate amyloidogenic fragments (PubMed : 17251185, PubMed : 7639763). Also acts as a regulator of cannabinoid signaling pathway by mediating degradation of hemopressin, an antagonist peptide of the cannabinoid receptor CNR1 (By similarity).

See full target information Thimet oligopeptidase

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental cell 60:2744-2760.e9 PubMed40460835

2025

ATG9A and ARFIP2 cooperate to control PI4P levels for lysosomal repair.

Applications

Unspecified application

Species

Unspecified reactive species

Stefano De Tito,Eugenia Almacellas,Daniel Dai Yu,Emily Millard,Wenxin Zhang,Cecilia de Heus,Christophe Queval,Javier H Hervás,Enrica Pellegrino,Ioanna Panagi,Ditte Fogde,Teresa L M Thurston,Judith Klumperman,Maximiliano Gutierrez,Sharon A Tooze

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thimet Oligopeptidase antibody [EPR7651] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-Thimet Oligopeptidase antibody [EPR7651] - BSA and Azide free (AB249073)

Western blot - Anti-Thimet Oligopeptidase antibody [EPR7651] - BSA and Azide free (AB249073)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise