Anti-Thiophosphate ester antibody [51-8] is a rabbit monoclonal antibody that is used to detect Thiophosphate ester in Western Blot.
- Recombinant format for unrivaled batch-batch consistency
- Cited in over 80 publications
Images
![Western blot - Anti-Thiophosphate ester antibody [51-8] (AB92570), expandable thumbnail](https://content.abcam.com/products/images/thiophosphate-ester-antibody-51-8-ab92570--western-blot-img125130.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thiophosphate ester antibody [51-8] (AB92570)")
Publications
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- Nature communications 14:80752023Enhanced SREBP2-driven cholesterol biosynthesis by PKCλ/ι deficiency in intestinal epithelial cells promotes aggressive serrated tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Muta,Juan F Linares,Anxo Martinez-Ordoñez,Angeles Duran,Tania Cid-Diaz,Hiroto Kinoshita,Xiao Zhang,Qixiu Han,Yuki Nakanishi,Naoko Nakanishi,Thekla Cordes,Gurpreet K Arora,Marc Ruiz-Martinez,Miguel Reina-Campos,Hiroaki Kasashima,Masakazu Yashiro,Kiyoshi Maeda,Ana Albaladejo-Gonzalez,Daniel Torres-Moreno,José García-Solano,Pablo Conesa-Zamora,Giorgio Inghirami,Christian M Metallo,Timothy F Osborne,Maria T Diaz-Meco,Jorge MoscatPubMed 38092754
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e23023682023Targeting CBX3 with a Dual BET/PLK1 Inhibitor Enhances the Antitumor Efficacy of CDK4/6 Inhibitors in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuaiyuan Liang,Chunguang Yang,Ruijiang Zeng,Yingqiu Song,Jianxi Wang,Wei Xiong,Binyuan Yan,Xin JinPubMed 37949681
- eLife 12:2023Analysis of CDPK1 targets identifies a trafficking adaptor complex that regulates microneme exocytosis in .Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlex W Chan,Malgorzata Broncel,Eden Yifrach,Nicole R Haseley,Sundeep Chakladar,Elena Andree,Alice L Herneisen,Emily Shortt,Moritz Treeck,Sebastian LouridoPubMed 37933960
- aBIOTECH 4:185-2012023DNA methylation-dependent epigenetic regulation of virulence in plants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYun-Ya Chen,Chen Zhu,Jian-Hua Zhao,Ting Liu,Feng Gao,Ying-Chao Zhang,Cheng-Guo DuanPubMed 37970467
- Science advances 9:eadf86982023CDK12 and Integrator-PP2A complex modulates LEO1 phosphorylation for processive transcription elongation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Qiu,Zhinang Yin,Honghong Wang,Lingyu Lei,Conghui Li,Yali Cui,Rong Dai,Peiyuan Yang,Ying Xiang,Qiuzi Li,Junhui Lv,Zhuang Hu,Min Chen,Hai-Bing Zhou,Pingping Fang,Ruijing Xiao,Kaiwei LiangPubMed 37205756
- Scientific reports 13:62782023In vitro kinase assay reveals ADP-heptose-dependent ALPK1 autophosphorylation and altered kinase activity of disease-associated ALPK1 mutants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiego García-Weber,Anne-Sophie Dangeard,Veronica Teixeira,Martina Hauke,Alexis Carreaux,Christine Josenhans,Cécile ArrieumerlouPubMed 37072480
- Molecular & cellular proteomics : MCP 22:1005222023Chemical Genetic Identification of PKC Epsilon Substrates in Mouse Brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael P Dugan,Laura B Ferguson,Nicholas T Hertz,Robert J Chalkley,Alma L Burlingame,Kevan M Shokat,Peter J Parker,Robert O MessingPubMed 36863607
- Life science alliance 6:2022Branch point strength controls species-specific alternative splicing and regulates LTP.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndreas Franz,A Ioana Weber,Marco Preußner,Nicole Dimos,Alexander Stumpf,Yanlong Ji,Laura Moreno-Velasquez,Anne Voigt,Frederic Schulz,Alexander Neumann,Benno Kuropka,Ralf Kühn,Henning Urlaub,Dietmar Schmitz,Markus C Wahl,Florian HeydPubMed 36543542
- Life science alliance 6:2022Loss of NDR1/2 kinases impairs endomembrane trafficking and autophagy leading to neurodegeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFlavia Roşianu,Simeon R Mihaylov,Noreen Eder,Antonie Martiniuc,Suzanne Claxton,Helen R Flynn,Shamsinar Jalal,Marie-Charlotte Domart,Lucy Collinson,Mark Skehel,Ambrosius P Snijders,Matthias Krause,Sharon A Tooze,Sila K UltanirPubMed 36446521
- Nature communications 13:72812022ARID1A loss induces polymorphonuclear myeloid-derived suppressor cell chemotaxis and promotes prostate cancer progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNi Li,Qiuli Liu,Ying Han,Siyu Pei,Bisheng Cheng,Junyu Xu,Xiang Miao,Qiang Pan,Hanling Wang,Jiacheng Guo,Xuege Wang,Guoying Zhang,Yannan Lian,Wei Zhang,Yi Zang,Minjia Tan,Qintong Li,Xiaoming Wang,Yichuan Xiao,Guohong Hu,Jun Jiang,Hai Huang,Jun QinPubMed 36435834
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | |
|---|---|
| Chemical | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Chemical | Dilution info 1/5000 | Notes - |
Associated Products
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1 product for Alternative Version
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Anti-Thiophosphate ester antibody [51-8] is a rabbit monoclonal antibody that is used to detect Thiophosphate ester in Western Blot.
- Recombinant format for unrivaled batch-batch consistency
- Cited in over 80 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- 51-8
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is specific to thiophosphorylated amino acids.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco.
It is possible to use ab92570 with ATP-gamma-S, Kinase substrate ab138911 as a kinase reaction reagent for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).
After the kinase of interest has accepted ATP-γ-S (ATP-gamma-S, Kinase substrate ab138911), p-Nitrobenzyl mesylate (p-Nitrobenzyl mesylate, Alkylation reagent ab138910) can be used to alkylate the thiophospholyation site on the substrates. ab92570 is introduced to identify the tagged substrates.
Anti-Thiophosphate ester antibody [51-8] (ab92570) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in WB.
Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Thiophosphate ester antibody [51-8] (ab92570) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Thiophosphate ester antibody [51-8] (ab92570) has been cited over 86 times in peer reviewed journals and is trusted by the scientific community.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Thiophosphate ester antibody [51-8] (ab92570), expandable thumbnail](https://content.abcam.com/products/images/thiophosphate-ester-antibody-51-8-ab92570--western-blot-img125130.jpg "Western blot - Anti-Thiophosphate ester antibody [51-8] (ab92570)")
Western blot - Anti-Thiophosphate ester antibody [51-8] (ab92570)
In vitro kinase reaction was carried out for 30 minutes at room temperature. PNBM alkylation (2.5mM) was for 2 hours at room temperature. Reaction products were analyzed by western blot with thiophosphate ester specific ab92570 at 1/5000, alpha-GST antibody confirms equal loading.
All lanes: Western blot - Anti-Thiophosphate ester antibody [51-8] (ab92570) at 1/5000 dilution
Lane 1: JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug)
Lane 2: JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using alkylation with PNBM (2.5mM).
Lane 3: JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S
Lane 4: JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S followed by alkylation with PNBM (2.5mM).
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