AB92570

Anti-Thiophosphate ester antibody [51-8]

RabMAb
Recombinant
What is this?

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(148 Publications)

Anti-Thiophosphate ester antibody [51-8] (ab92570) is a rabbit monoclonal antibody detecting Thiophosphate ester in Western Blot.

- Biophysical QC for unrivalled batch-batch consistency
- Over 80 publications
- Trusted since 2010

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Western blot - Anti-Thiophosphate ester antibody [51-8] (AB92570)

In vitro kinase reaction was carried out for 30 minutes at room temperature. PNBM alkylation (2.5mM) was for 2 hours at room temperature. Reaction products were analyzed by western blot with thiophosphate ester specific ab92570 at 1/5000, alpha-GST antibody confirms equal loading.

All lanes:

Western blot - Anti-Thiophosphate ester antibody [51-8] (ab92570) at 1/5000 dilution

Lane 1:

JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug)

Lane 2:

JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using alkylation with PNBM (2.5mM).

Lane 3:

JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S

Lane 4:

JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S followed by alkylation with PNBM (2.5mM).

false

Carrier free

Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free
Unconjugated

Anti-Thiophosphate ester antibody [51-8]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

51-8

Isotype

IgG

Carrier free

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to thiophosphorylated amino acids.

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Reactivity data

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Product details

This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco.

It is possible to use ab92570 with ab138911 as a kinase reaction reagent for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007).

After the kinase of interest has accepted ATP-γ-S (ab138911), p-Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. ab92570 is introduced to identify the tagged substrates.

Product Specifications
Anti-Thiophosphate ester antibody [51-8] (ab92570) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in WB.

Quality and Validation
Abcam's high quality manufacturing and validation processes ensure Anti-Thiophosphate ester antibody [51-8] (ab92570) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Thiophosphate ester antibody [51-8] (ab92570) has been cited over 86 times in peer reviewed journals and is trusted by the scientific community.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The thiophosphate ester often referred to as ATP gamma S is a structural analog of adenosine triphosphate (ATP) where the oxygen is substituted with sulfur at the gamma position. This modification results in resistance to hydrolysis making it useful for studying ATP-dependent processes. Although the exact mass of thiophosphate ester varies depending on the specific derivative it is widely utilized in various cellular and organ systems due to its involvement as an ATP analog. The compound is not expressible in living organisms but it is valuable in experimental designs in laboratory settings.

Biological function summary

The role of the thiophosphate ester centers around its use as a tool for modulating enzymatic activity in cellular processes. It often functions as part of complex biochemical assays to investigate phosphorylation mechanisms. As a gamma phosphate analog it non-hydrolyzably activates kinases and other ATP-binding proteins providing insights into their dynamics and interactions within cellular structures. Researchers commonly use it in studies involving protein phosphorylation and signal transduction.

Pathways

The thiophosphate ester plays a significant role in ATP-dependent phosphorylation pathways particularly those involving protein kinases. Its analog can mimic ATP in signal transduction pathways which leads to sustained activation of pathways like MAPK/ERK important for cell proliferation and differentiation. It shares relations with various kinases such as PKA and PKC which regulate diverse cellular responses. This serves to illustrate how its presence aids in understanding the regulation and execution of these pathways by inhibiting or prolonging their activity.

Thiophosphate ester analogs help researchers examine aberrant kinase signaling linked to cancer and neurodegenerative diseases. By stabilizing interactions with proteins such as p53 in cancer pathways it is possible to evaluate the role of faulty phosphorylation in tumorigenesis. In the context of neurodegenerative disorders the ester assists in investigating abnormal signal transduction pathways that may lead to conditions like Alzheimer’s disease. By providing a platform for studying protein interactions it contributes to the understanding of how improper regulation can lead to disease development and progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Thiophosphate ester

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Publications (148)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadu9555 PubMed40712037

2025

DNA damage response pathway regulates Nrf2 in response to oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-You Jiang,Qi-Qiang Guo,Shan-Shan Wang,Ran Guo,Yu Zou,Jing-Wei Liu,Yan-Ling Feng,Yang Guo,Yu-Han Li,Xi-Yan Liu,Xin-Yue Zhang,Shuang Hao,Xiao-Xu Wu,Meng-Han Li,Ao Liu,Chun-Lu Li,Wen-Dong Guo,Hong-De Xu,Xiao-Yu Song,Toren Finkel,Liu Cao

Science advances 11:eadu2840 PubMed40446045

2025

Differential phosphorylation of receptor kinase SlLYK4 mediates immune responses to bacterial and fungal pathogens in tomato.

Applications

Unspecified application

Species

Unspecified reactive species

Wanting Zhu,Sen Cao,Mengling Huang,Pengyue Li,Jingjing Ke,Ai Xu,Yang Lin,Jiatao Xie,Jiasen Cheng,Yanping Fu,Daohong Jiang,Xiao Yu,Bo Li

EMBO reports 26:3411-3444 PubMed40410380

2025

Dynamic SAS-6 phosphorylation aids centrosome duplication and elimination in C. elegans oogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Feifei Qi,Shanshan Yin,Xiangrui Yang,Ning Ju,Bohan Liu,Xing Zhang,Zixuan Zhu,Li Ji,Fuxin Zhang,Li Zhao,Ruoxi Wang,Min Liu,Liangran Zhang,Huijie Zhao,Jun Zhou,Jinmin Gao

Frontiers in cell and developmental biology 13:1554958 PubMed40365021

2025

Omic AI reveals new autophagy regulators from the Atg1 interactome in .

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Han,Shanshan Fu,Dachao Tang,Yuting Chen,Dan Liu,Zihao Feng,Yujie Gou,Chi Zhang,Weizhi Zhang,Leming Xiao,Jiayi Zhang,Cong Yi,Yu Xue,Di Peng

Cell communication and signaling : CCS 23:194 PubMed40269955

2025

CDO1 phosphorylation is required for IL-6-induced tumor cell proliferation through governing cysteine availability.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Zhe Zhao,Hongping Ye,Dan Li,Xiaoke Huang,Jong-Ho Lee,Rui Liu

Theranostics 15:2814-2833 PubMed40083925

2025

FBXW7/GSK3β-mediated proline-rich 11 degradation promotes oxidative DNA damage and inhibits tumor progression in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Siming Chen,Kangping Xiong,Jianmin Liu,Shijie Yao,Mingxing Li,Jingtian Yu,Gang Wang,Sheng Tu,Wan Jin,Jiageng Shi,Yu Xiao,Yi Zhang,Kaiyu Qian,Lingao Ju,Xinghuan Wang

Nature communications 16:2454 PubMed40074748

2025

Salt stress activates the CDK8-AHL10-SUVH2/9 module to dynamically regulate salt tolerance in Arabidopsis.

Applications

Unspecified application

Species

Unspecified reactive species

Pengcheng Guo,Leelyn Chong,Zhixin Jiao,Rui Xu,Qingfeng Niu,Yingfang Zhu

Science advances 11:eadt4548 PubMed40073115

2025

MLX phosphorylation stabilizes the ChREBP-MLX heterotetramer on tandem E-boxes to control carbohydrate and lipid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Carla E Cadena Del Castillo,Onur Deniz,Femke van Geest,Lore Rosseels,Ingrid Stockmans,Marius Robciuc,Sebastien Carpentier,Bettina K Wölnerhanssen,Anne Christin Meyer-Gerspach,Ralph Peterli,Ville Hietakangas,Mitsugu Shimobayashi

Nature communications 16:1738 PubMed39966410

2025

Macrophage SUCLA2 coupled glutaminolysis manipulates obesity through AMPK.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Peng,Haowen Jiang,Liya Jing,Wenhua Yang,Xiaotong Guan,Hanlin Wang,Sike Yu,Yutang Cao,Min Wang,Huan Ma,Zan Lv,Hongyu Gu,Chunmei Xia,Xiaozhen Guo,Bin Sun,Aili Wang,Cen Xie,Wenbiao Wu,Luyiyi Lu,Jiayi Song,Saifei Lei,Rui Wu,Yi Zang,Erjiang Tang,Jia Li

Stem cell reports 20:102397 PubMed39919750

2025

ERK phosphorylates ESRRB to regulate the self-renewal and differentiation of mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaowei Duan,Qingye Zhang,Lulu Gao,Bin Ling,Xiaoling Du,Lingyi Chen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thiophosphate ester antibody [51-8]

Western blot - Anti-Thiophosphate ester antibody [51-8] (AB92570)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Immunogen

Specificity

Complementary Products

Biochemicals

Biochemicals

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (148)

Product promise