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AB239919

Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free

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(3 Publications)

Rabbit Recombinant Monoclonal Thiophosphate ester antibody. Carrier free. Suitable for WB and reacts with Chemical samples. Cited in 3 publications.
1 Images
Western blot - Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free (AB239919)
  • WB

Unknown

Western blot - Anti-Thiophosphate ester antibody [51-8] - BSA and Azide free (AB239919)

This data was developed using ab92570, the same antibody clone in a different buffer formulation.

In vitro kinase reaction was carried out for 30 minutes at room temperature. PNBM alkylation (2.5mM) was for 2 hours at room temperature. Reaction products were analyzed by western blot with thiophosphate ester specific ab92570 at 1/5000, alpha-GST antibody confirms equal loading.

All lanes:

Western blot - Anti-Thiophosphate ester antibody [51-8] (<a href='/en-us/products/primary-antibodies/thiophosphate-ester-antibody-51-8-ab92570'>ab92570</a>) at 1/5000 dilution

Lane 1:

JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug)

Lane 2:

JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using alkylation with PNBM (2.5mM).

Lane 3:

JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S

Lane 4:

JNK1 protein (10ng) incubated with c-jun-GST substrate (1ug) using ATP-gamma-S followed by alkylation with PNBM (2.5mM).

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

51-8

Isotype

IgG

Carrier free

Yes

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific to thiophosphorylated amino acids.

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Complementary Products

Biochemicals

AB138911

ATP-gamma-S, Kinase substrate

Chemical Structure - ATP-gamma-S, Kinase substrate (AB138911)

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Biochemicals

AB138910

p-Nitrobenzyl mesylate, Alkylation reagent

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Primary Antibodies

AB92570

Anti-Thiophosphate ester antibody [51-8]

RabMAb|Recombinant

Western blot - Anti-Thiophosphate ester antibody [51-8] (AB92570)

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Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

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Reactivity data

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Product details

ab239919 is the carrier-free version of ab92570.

This antibody was developed as part of a collaboration between Abcam and the lab of Dr. Kevan Shokat at University of California at San Francisco. It is possible to use ab92570 with ab138911 as a kinase reaction reagent for the identification of direct kinase substrates as mentioned in Allen JJ et al. Nat Methods 4:511-6 (2007). After the kinase of interest has accepted ATP-γ-S (ab138911), p -Nitrobenzyl mesylate (ab138910) can be used to alkylate the thiophospholyation site on the substrates. ab92570 is introduced to identify the tagged substrates.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The thiophosphate ester often referred to as ATP gamma S is a structural analog of adenosine triphosphate (ATP) where the oxygen is substituted with sulfur at the gamma position. This modification results in resistance to hydrolysis making it useful for studying ATP-dependent processes. Although the exact mass of thiophosphate ester varies depending on the specific derivative it is widely utilized in various cellular and organ systems due to its involvement as an ATP analog. The compound is not expressible in living organisms but it is valuable in experimental designs in laboratory settings.
Biological function summary

The role of the thiophosphate ester centers around its use as a tool for modulating enzymatic activity in cellular processes. It often functions as part of complex biochemical assays to investigate phosphorylation mechanisms. As a gamma phosphate analog it non-hydrolyzably activates kinases and other ATP-binding proteins providing insights into their dynamics and interactions within cellular structures. Researchers commonly use it in studies involving protein phosphorylation and signal transduction.

Pathways

The thiophosphate ester plays a significant role in ATP-dependent phosphorylation pathways particularly those involving protein kinases. Its analog can mimic ATP in signal transduction pathways which leads to sustained activation of pathways like MAPK/ERK important for cell proliferation and differentiation. It shares relations with various kinases such as PKA and PKC which regulate diverse cellular responses. This serves to illustrate how its presence aids in understanding the regulation and execution of these pathways by inhibiting or prolonging their activity.

Thiophosphate ester analogs help researchers examine aberrant kinase signaling linked to cancer and neurodegenerative diseases. By stabilizing interactions with proteins such as p53 in cancer pathways it is possible to evaluate the role of faulty phosphorylation in tumorigenesis. In the context of neurodegenerative disorders the ester assists in investigating abnormal signal transduction pathways that may lead to conditions like Alzheimer’s disease. By providing a platform for studying protein interactions it contributes to the understanding of how improper regulation can lead to disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Thiophosphate ester
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed39412391

2024

Negative regulation of APC/C activation by MAPK-mediated attenuation of Cdc20 under stress.

Applications

Unspecified application

Species

Unspecified reactive species

Li Sun,Xuejin Chen,Chunlin Song,Wenjing Shi,Libo Liu,Shuang Bai,Xi Wang,Jiali Chen,Chengyu Jiang,Shuang-Min Wang,Zhou-Qing Luo,Ruiwen Wang,Yamei Wang,Quan-Wen Jin

Nature communications 12:4906 PubMed34385456

2021

An N-Cadherin 2 expressing epithelial cell subpopulation predicts response to surgery, chemotherapy and immunotherapy in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth H Gouin,Nathan Ing,Jasmine T Plummer,Charles J Rosser,Bassem Ben Cheikh,Catherine Oh,Stephanie S Chen,Keith Syson Chan,Hideki Furuya,Warren G Tourtellotte,Simon R V Knott,Dan Theodorescu

eLife 9: PubMed32915139

2020

Stress-activated MAPK signaling controls fission yeast actomyosin ring integrity by modulating formin For3 levels.

Applications

Unspecified application

Species

Unspecified reactive species

Elisa Gómez-Gil,Rebeca Martín-García,Jero Vicente-Soler,Alejandro Franco,Beatriz Vázquez-Marín,Francisco Prieto-Ruiz,Teresa Soto,Pilar Pérez,Marisa Madrid,Jose Cansado
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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