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AB240292

Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal Thioredoxin 2 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human samples. Cited in 1 publication.

View Alternative Names

TRX2, TXN2, MTRX, Mt-Trx, Thioredoxin-2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free (AB240292)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free (AB240292)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185544).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free (AB240292)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free (AB240292)

Immunofluorescent analysis of 4% paraformaldehyde fixed HeLa cells labeling Thioredoxin 2 with ab185544 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185544).

Flow Cytometry (Intracellular) - Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free (AB240292)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free (AB240292)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed K562 cells labeling Thioredoxin 2 with ab185544 at 1/150 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185544).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free (AB240292)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin 2 antibody [EPR15225] - BSA and Azide free (AB240292)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling Thioredoxin 2 with ab185544 at 1/500 dilution. Counter stain Hematoxylin.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185544).

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Unconjugated

    Anti-Thioredoxin 2 antibody [EPR15225]

  • 660 APC

    APC Anti-Thioredoxin 2 antibody [EPR15225]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-Thioredoxin 2 antibody [EPR15225]

  • 565 Alexa Fluor® 555

    Alexa Fluor® 555 Anti-Thioredoxin 2 antibody [EPR15225]

  • 603 Alexa Fluor® 568

    Alexa Fluor® 568 Anti-Thioredoxin 2 antibody [EPR15225]

  • 617 Alexa Fluor® 594

    Alexa Fluor® 594 Anti-Thioredoxin 2 antibody [EPR15225]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Thioredoxin 2 antibody [EPR15225]

  • 775 Alexa Fluor® 750

    Alexa Fluor® 750 Anti-Thioredoxin 2 antibody [EPR15225]

  • HRP

    HRP Anti-Thioredoxin 2 antibody [EPR15225]

  • 578 PE

    PE Anti-Thioredoxin 2 antibody [EPR15225]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15225

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

IHC-P, WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-low-endotoxin-azide-free-ab199376'>ab199376</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab240292 is the carrier-free version of ab185544.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin 2 also known as TXN2 is a protein that functions mainly as an oxidoreductase. It facilitates the reduction of other proteins through cysteine thiol-disulfide exchange which is important for maintaining cellular redox balance. This protein exists in the mitochondria where it is expressed ubiquitously across tissues. Thioredoxin 2 has a molecular mass of approximately 18 kDa. The presence of TXN2 in all cellular compartments suggests its importance in various cellular processes involving oxidative stress response.
Biological function summary

Thioredoxin 2 participates in regulating mitochondrial redox homeostasis ensuring the protection of mitochondrial DNA against oxidative damage. It acts as a part of the mitochondrial thioredoxin system which includes thioredoxin reductase and NADPH as components. This system is important for the control of mitochondrial reactive oxygen species (ROS) and supports mitochondrial function by facilitating the synthesis of deoxyribonucleotides. The protein therefore plays an important role in cellular antioxidant defenses and in modulating mitochondrial apoptotic pathways.

Pathways

The involvement of Thioredoxin 2 is pivotal in several cellular signaling cascades including the antioxidant defense and apoptosis pathways. Within the redox signaling pathways TXN2 works in conjunction with cytochrome c and caspases to regulate apoptosis and prevent uncontrolled cell death. In pathways related to cellular oxidative stress Thioredoxin 2 interacts closely with glutathione to mediate cellular responses to oxidative damage maintaining balance between the production and removal of ROS.

The dysregulation of Thioredoxin 2 correlates with various human conditions such as cancer and neurodegenerative diseases. In cancer alterations in TXN2 levels can affect cell survival and proliferation often by interacting with other antioxidant proteins like peroxiredoxins. For neurodegenerative diseases an imbalance in Thioredoxin 2 activity contributes to neuronal damage through disrupted oxidative stress regulation. This highlights TXN2's potential as a therapeutic target for disorders connected to oxidative stress imbalances.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Important for the control of mitochondrial reactive oxygen species homeostasis, apoptosis regulation and cell viability (PubMed : 12032145, PubMed : 12080052, PubMed : 26626369) Is involved in various redox reactions including the reduction of protein disulfide bonds, through the reversible oxidation of its active center dithiol to a disulfide (By similarity).
See full target information TXN2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

European journal of histochemistry : EJH 65: PubMed34587716

2021

Long noncoding RNA Meg3 mediates ferroptosis induced by oxygen and glucose deprivation combined with hyperglycemia in rat brain microvascular endothelial cells, through modulating the p53/GPX4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Yan Huang,Pingping Xia,Fan Zhang,Longyan Li,E Wang,Qulian Guo,Zhi Ye
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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