AB133524

Anti-Thioredoxin / TRX antibody [EPR6110]

Name: Anti-Thioredoxin / TRX antibody [EPR6110] (ab133524) | Abcam
Brand: Abcam Limited
SKU: AB133524
Price: 400 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
Lab Essentials
What is this?

(1 Review)

(21 Publications)

Rabbit Recombinant Monoclonal Thioredoxin / TRX antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 21 publications.

View Alternative Names

TRDX, TRX, TRX1, TXN, Thioredoxin, Trx, ATL-derived factor, Surface-associated sulphydryl protein, ADF, SASP

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR6110] (AB133524)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR6110] (AB133524)

Immunohistochemistry analysis of Paraffin Embedded Human colon tissue labelling Thioredoxin/TRX with ab133524 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Thioredoxin / TRX antibody [EPR6110] (AB133524)

All lanes:

Western blot - Anti-Thioredoxin / TRX antibody [EPR6110] (ab133524) at 1/10000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

THP1 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Lane 4:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

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OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6110] (AB133524)

OI-RD Scanning

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OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6110] (AB133524)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-Thioredoxin / TRX antibody [EPR6110] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6110

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin (TRX) also known as TXN or Trx is a small redox protein with a molecular mass of approximately 12 kDa. It acts as an oxidoreductase enzyme facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxin is ubiquitously expressed across various tissues indicating its function in a broad range of cellular activities. The protein comprises an active site with a conserved Cys-Gly-Pro-Cys sequence critical for its reducing activity.

Biological function summary

Thioredoxin influences cellular redox homeostasis and plays direct roles in regulating cell growth and apoptosis. It participates as a major reductant in cells influencing transcription factors and enzymatic activities that depend on thiol-disulfide exchanges. Thioredoxin can interact with other components like thioredoxin reductase and NADPH to form the thioredoxin system a powerful antioxidant defense mechanism. Diseases typically arise from abnormal regulation of this system highlighting its influence on cellular survival and proliferation.

Pathways

Thioredoxin integrates into significant signaling and metabolic processes. It is an important player in the cellular response to oxidative stress and participates in signaling pathways such as the MAPK and apoptosis pathways. In these pathways thioredoxin reduces the oxidative stress transcription factor AP-1 influencing cell fate decisions. Its interaction with thioredoxin-interacting protein (TXNIP) further modulates cellular responses to oxidative environments.

Altered thioredoxin activity associates with cancer and neurodegenerative diseases. Its overexpression links to tumor progression where it promotes survival and resistance to apoptosis. Additionally in the context of neurodegenerative disorders thioredoxin’s interaction with amyloid-beta peptides implicates it in Alzheimer’s disease by mitigating oxidative stress damage. Through these conditions its role as a protector against oxidative stress remains pivotal.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed : 17182577, PubMed : 19032234, PubMed : 2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed : 16408020, PubMed : 17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed : 11118054, PubMed : 9108029).. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

See full target information TXN

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Publications (21)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:28829 PubMed40770005

2025

Explore the role of CBS in stomach adenocarcinoma based on the sulfur-containing amino acid metabolism network.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Zhang,Yingchao Liu,Yajuan Lu,Aoli Deng,Jinghao Cao,Hangqi Huang,Cuiyun Wu,Jing Du,Yanchun Li,Jun Xia

Materials today. Bio 32:101914 PubMed40520563

2025

IL-15 functionalized biomimetic hybrid mRNA vaccine for enhanced NSCLC immunotherapy via synergistic activation of T cells and NK cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jueshuo Guo,Li Peng,Penglong Ma,Yaping Mai,Ting Gao,Na Yu,Wenbao Zuo,Jianhong Yang

Cellular & molecular biology letters 30:61 PubMed40405062

2025

IL-6-induced long noncoding RNA MIR3142HG promotes tumorigenesis by interacting with thioredoxin-1 and STAT3 in human colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Daoquan Fang,Qian Feng,Baojian Zhou,Yangyang Liu,Yichu Lian,Yihui Zhang,Dichen Yang,Xintong Liu,Xiaomeng Shi,Wuhua Ni,Lei Jiang

Journal of inflammation research 17:371-385 PubMed38260812

2024

Heme-Inducing Endothelial Pyroptosis Plays a Key Role in Radiofrequency Ablation of Hepatic Hemangioma Leading to Systemic Inflammatory Response Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Changyu Yao,Jian Kong,Fei Xu,Shaohong Wang,Shilun Wu,Wenbing Sun,Jun Gao

Environmental toxicology 39:1936-1950 PubMed38064254

2023

Bardoxolone methyl attenuates doxorubicin-induced cardiotoxicity by inhibiting the TXNIP-NLRP3 pathway through Nrf2 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zhang,Chao Shi,Zhuoya Yao,Shaohuan Qian

Theranostics 13:4730-4744 PubMed37771783

2023

Nuclear translocation of thioredoxin-1 promotes colorectal cancer development via modulation of the IL-6/STAT3 signaling axis through interaction with STAT3.

Applications

Unspecified application

Species

Unspecified reactive species

Aihua Wu,Daoquan Fang,Yangyang Liu,Xiaomeng Shi,Zuyue Zhong,Baojian Zhou,Lechi Ye,Xuecheng Sun,Lei Jiang

Molecular medicine reports 28: PubMed37232340

2023

Thioredoxin1 is a target to attenuate diabetes‑induced RPE cell dysfunction in human ARPE19 cells by alleviating oxidative stress.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Qi,Tianhe Liu,Jiasu Liu,Qiufeng Teng,Zhongping Ma,Shengnan Wang,Shihui Wen,Chenghong Zhang,Xiang Ren,Hui Kong,Li Kong

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22943 PubMed37104068

2023

Thioredoxin facilitates hepatocellular carcinoma stemness and metastasis by increasing BACH1 stability to activate the AKT/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengmeng Wang,Lu Zhang,Manqing Cao,Zhou Fu,Huaqi Wang,Su Zhang,Keyun Zhu,Zhenyu Hou,Jinfang Cui,Ping Yue,Hua Guo,Ti Zhang

Cancer research 82:3486-3498 PubMed35916672

2022

Selenium Modulates Cancer Cell Response to Pharmacologic Ascorbate.

Applications

Unspecified application

Species

Unspecified reactive species

Connor S R Jankowski,Joshua D Rabinowitz

NeuroSci 3:166-185 PubMed39483368

2022

Localization of Thioredoxin-Interacting Protein in Aging and Alzheimer's Disease Brains.

Applications

Unspecified application

Species

Unspecified reactive species

Haruka Tsubaki,Anarmaa Mendsaikhan,Undral Buyandelger,Ikuo Tooyama,Douglas G Walker

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thioredoxin / TRX antibody [EPR6110]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thioredoxin / TRX antibody [EPR6110] (AB133524)

Western blot - Anti-Thioredoxin / TRX antibody [EPR6110] (AB133524)

OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6110] (AB133524)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (21)

Product promise