AB109385

Anti-Thioredoxin / TRX antibody [EPR6111]

RabMAb
Recombinant
KO Validated
Lab Essentials
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(8 Publications)

Rabbit Recombinant Monoclonal Thioredoxin / TRX antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

View Alternative Names

TRDX, TRX, TRX1, TXN, Thioredoxin, Trx, ATL-derived factor, Surface-associated sulphydryl protein, ADF, SASP

3 Images

Lab

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (AB109385)

All lanes:

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 5:

Mouse brain lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 7:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

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Lab

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (AB109385)

All lanes:

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (ab109385) at 1/1000 dilution

All lanes:

His-tagged human Thioredoxin recombinant protein (aa 1-105)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

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CiteAb

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (AB109385)

Thioredoxin / TRX western blot using anti-Thioredoxin / TRX antibody [EPR6111] ab109385. Publication image and figure legend from Bouitbir, J., Panajatovic, M. V., et al., 2020, Front Pharmacol, PubMed 32792947.

ab109385 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab109385 please see the product overview.

Antioxidative defenses in C2C12 myotubes and human RD cells. (A) Western blots showing the expression levels of SOD1, SOD2, TRX1, TRX2, and GAPDH in C2C12 myotubes. (B) The graph shows the quantification of protein expression normalized against GAPDH. (C) The mRNA expression of genes involved in the antioxidative defense in RD cells. Data represent the mean ± SEM of three independent experiments. Treatments with TKIs were compared to 0.1% DMSO control with one-way ANOVA followed by Dunnett's post-test. *p < 0.05 versus 0.1% DMSO control. Ima, imatinib; Das, dasatinib; SOD, superoxide dismutase; TRX, thioredoxin.

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Carrier free

Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6111

Isotype

IgG

Carrier free

Reacts with

Human, Rat, Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin (TRX) also known as TXN or Trx is a small redox protein with a molecular mass of approximately 12 kDa. It acts as an oxidoreductase enzyme facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxin is ubiquitously expressed across various tissues indicating its function in a broad range of cellular activities. The protein comprises an active site with a conserved Cys-Gly-Pro-Cys sequence critical for its reducing activity.

Biological function summary

Thioredoxin influences cellular redox homeostasis and plays direct roles in regulating cell growth and apoptosis. It participates as a major reductant in cells influencing transcription factors and enzymatic activities that depend on thiol-disulfide exchanges. Thioredoxin can interact with other components like thioredoxin reductase and NADPH to form the thioredoxin system a powerful antioxidant defense mechanism. Diseases typically arise from abnormal regulation of this system highlighting its influence on cellular survival and proliferation.

Pathways

Thioredoxin integrates into significant signaling and metabolic processes. It is an important player in the cellular response to oxidative stress and participates in signaling pathways such as the MAPK and apoptosis pathways. In these pathways thioredoxin reduces the oxidative stress transcription factor AP-1 influencing cell fate decisions. Its interaction with thioredoxin-interacting protein (TXNIP) further modulates cellular responses to oxidative environments.

Altered thioredoxin activity associates with cancer and neurodegenerative diseases. Its overexpression links to tumor progression where it promotes survival and resistance to apoptosis. Additionally in the context of neurodegenerative disorders thioredoxin’s interaction with amyloid-beta peptides implicates it in Alzheimer’s disease by mitigating oxidative stress damage. Through these conditions its role as a protector against oxidative stress remains pivotal.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed : 17182577, PubMed : 19032234, PubMed : 2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed : 16408020, PubMed : 17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed : 11118054, PubMed : 9108029).. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

See full target information TXN

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0286903 PubMed37310976

2023

The angiotensin receptor neprilysin inhibitor LCZ696 attenuates renal fibrosis via ASK1/JNK/p38 MAPK-mediated apoptosis in unilateral ureteral obstruction.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ding,Sheng Cui,Song Yu Li,Lin Yan Cui,Qi Yan Nan,Xue Jing Lin,Mei Ying Xuan,Jian Jin,Shang Guo Piao,Yu Ji Jiang,Hai Lan Zheng,Ji Zhe Jin,Byung Ha Chung,Chul Woo Yang,Jing Hao Cui,Can Li

Investigative ophthalmology & visual science 64:16 PubMed36881408

2023

SIRT1 Inhibits High Glucose-Induced TXNIP/NLRP3 Inflammasome Activation and Cataract Formation.

Applications

Unspecified application

Species

Unspecified reactive species

Lili Lian,Zhenmin Le,Zhenzhen Wang,Ying-Ao Chen,Xiaodong Jiao,Hang Qi,J Fielding Hejtmancik,Xiaoyin Ma,Qinxiang Zheng,Yueping Ren

Experimental and therapeutic medicine 25:144 PubMed36911380

2023

Gelatin methacryloyl hydrogel scaffold loaded with activated Schwann cells attenuates apoptosis and promotes functional recovery following spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Hao Yu,Peng Yu,Peng Peng,Chao Li,Zhenyang Xiang,Dexiang Ban

BMC cardiovascular disorders 21:603 PubMed34922451

2021

Glyoxal damages human aortic endothelial cells by perturbing the glutathione, mitochondrial membrane potential, and mitogen-activated protein kinase pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Ming-Zhang Xie,Chun Guo,Jia-Qi Dong,Jie Zhang,Ke-Tao Sun,Guang-Jian Lu,Lei Wang,De-Ying Bo,Lu-Yang Jiao,Guo-An Zhao

British journal of pharmacology 178:4608-4625 PubMed34265086

2021

Inhibition of HSP90 S-nitrosylation alleviates cardiac fibrosis via TGFβ/SMAD3 signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiyue Zhang,Yihua Zhang,Qing Miao,Zhiguang Shi,Lulu Hu,Shangmin Liu,Jie Gao,Shuang Zhao,Hongshan Chen,Zhengrong Huang,Yi Han,Yong Ji,Liping Xie

Redox biology 46:102065 PubMed34293554

2021

Lactate and glutamine support NADPH generation in cancer cells under glucose deprived conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Minfeng Ying,Duo You,Xiaobing Zhu,Limeng Cai,Siying Zeng,Xun Hu

Frontiers in pharmacology 11:1106 PubMed32792947

2020

Imatinib and Dasatinib Provoke Mitochondrial Dysfunction Leading to Oxidative Stress in C2C12 Myotubes and Human RD Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jamal Bouitbir,Miljenko Valentin Panajatovic,Theo Frechard,Noëmi Johanna Roos,Stephan Krähenbühl

Naunyn-Schmiedeberg's archives of pharmacology 393:991-1002 PubMed31811327

2019

Salvianolic acid A attenuated myocardial infarction-induced apoptosis and inflammation by activating Trx.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhou,Jinhuan Gao,Changpei Xiang,Zhenming Liu,Yi Zhang,Jingjing Zhang,Hongjun Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thioredoxin / TRX antibody [EPR6111]

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (AB109385)

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (AB109385)

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (AB109385)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

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