Skip to main content

Rabbit Recombinant Monoclonal Thioredoxin / TRX antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914), expandable thumbnail
  • Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914), expandable thumbnail
  • OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested
Recombinant fragment - Human
Tested

Tested
Tested

Species
Recombinant fragment - Human, Mouse, Rat, Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed:17182577, PubMed:19032234, PubMed:2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed:16408020, PubMed:17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed:11118054, PubMed:9108029). ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

Alternative names

TRDX, TRX, TRX1, TXN, Thioredoxin, Trx, ATL-derived factor, Surface-associated sulphydryl protein, ADF, SASP

Recommended products

Rabbit Recombinant Monoclonal Thioredoxin / TRX antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6111
Purification technique
Affinity purification Protein A
Dissociation constant
1.03 x 10-10 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab238914 is the carrier-free version of Anti-Thioredoxin / TRX antibody [EPR6111] ab109385.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Thioredoxin (TRX) also known as TXN or Trx is a small redox protein with a molecular mass of approximately 12 kDa. It acts as an oxidoreductase enzyme facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxin is ubiquitously expressed across various tissues indicating its function in a broad range of cellular activities. The protein comprises an active site with a conserved Cys-Gly-Pro-Cys sequence critical for its reducing activity.

Biological function summary

Thioredoxin influences cellular redox homeostasis and plays direct roles in regulating cell growth and apoptosis. It participates as a major reductant in cells influencing transcription factors and enzymatic activities that depend on thiol-disulfide exchanges. Thioredoxin can interact with other components like thioredoxin reductase and NADPH to form the thioredoxin system a powerful antioxidant defense mechanism. Diseases typically arise from abnormal regulation of this system highlighting its influence on cellular survival and proliferation.

Pathways

Thioredoxin integrates into significant signaling and metabolic processes. It is an important player in the cellular response to oxidative stress and participates in signaling pathways such as the MAPK and apoptosis pathways. In these pathways thioredoxin reduces the oxidative stress transcription factor AP-1 influencing cell fate decisions. Its interaction with thioredoxin-interacting protein (TXNIP) further modulates cellular responses to oxidative environments.

Associated diseases and disorders

Altered thioredoxin activity associates with cancer and neurodegenerative diseases. Its overexpression links to tumor progression where it promotes survival and resistance to apoptosis. Additionally in the context of neurodegenerative disorders thioredoxin’s interaction with amyloid-beta peptides implicates it in Alzheimer’s disease by mitigating oxidative stress damage. Through these conditions its role as a protector against oxidative stress remains pivotal.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (ab238914), expandable thumbnail

    Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (ab238914)

    This data was developed using Anti-Thioredoxin / TRX antibody [EPR6111] ab109385 the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (Anti-Thioredoxin / TRX antibody [EPR6111] ab109385) at 1/1000 dilution

    All lanes: His-tagged human Thioredoxin recombinant protein (aa 1-105)

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (ab238914), expandable thumbnail

    Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (ab238914)

    This data was developed using Anti-Thioredoxin / TRX antibody [EPR6111] ab109385 the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (Anti-Thioredoxin / TRX antibody [EPR6111] ab109385) at 1/1000 dilution

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

    Lane 5: Mouse brain lysate at 20 µg

    Lane 6: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

    Lane 7: C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 12 kDa

    Observed band size: 12 kDa

  • OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (ab238914), expandable thumbnail

    OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (ab238914)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com