AB238914

Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Thioredoxin / TRX antibody. Carrier free. Suitable for WB and reacts with Recombinant fragment - Human, Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

TRDX, TRX, TRX1, TXN, Thioredoxin, Trx, ATL-derived factor, Surface-associated sulphydryl protein, ADF, SASP

3 Images

Lab

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914)

This data was developed using ab109385 the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (<a href='/en-us/products/primary-antibodies/thioredoxin-trx-antibody-epr6111-ab109385'>ab109385</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

C2C12 (Mouse myoblasts myoblast) whole cell lysate at 20 µg

Lane 5:

Mouse brain lysate at 20 µg

Lane 6:

PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 7:

C6 (Rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Lab

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914)

This data was developed using ab109385 the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] (<a href='/en-us/products/primary-antibodies/thioredoxin-trx-antibody-epr6111-ab109385'>ab109385</a>) at 1/1000 dilution

All lanes:

His-tagged human Thioredoxin recombinant protein (aa 1-105)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Unconjugated

Anti-Thioredoxin / TRX antibody [EPR6111]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6111

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Rat, Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

ab238914 is the carrier-free version of ab109385.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thioredoxin (TRX) also known as TXN or Trx is a small redox protein with a molecular mass of approximately 12 kDa. It acts as an oxidoreductase enzyme facilitating the reduction of other proteins by cysteine thiol-disulfide exchange. Thioredoxin is ubiquitously expressed across various tissues indicating its function in a broad range of cellular activities. The protein comprises an active site with a conserved Cys-Gly-Pro-Cys sequence critical for its reducing activity.

Biological function summary

Thioredoxin influences cellular redox homeostasis and plays direct roles in regulating cell growth and apoptosis. It participates as a major reductant in cells influencing transcription factors and enzymatic activities that depend on thiol-disulfide exchanges. Thioredoxin can interact with other components like thioredoxin reductase and NADPH to form the thioredoxin system a powerful antioxidant defense mechanism. Diseases typically arise from abnormal regulation of this system highlighting its influence on cellular survival and proliferation.

Pathways

Thioredoxin integrates into significant signaling and metabolic processes. It is an important player in the cellular response to oxidative stress and participates in signaling pathways such as the MAPK and apoptosis pathways. In these pathways thioredoxin reduces the oxidative stress transcription factor AP-1 influencing cell fate decisions. Its interaction with thioredoxin-interacting protein (TXNIP) further modulates cellular responses to oxidative environments.

Altered thioredoxin activity associates with cancer and neurodegenerative diseases. Its overexpression links to tumor progression where it promotes survival and resistance to apoptosis. Additionally in the context of neurodegenerative disorders thioredoxin’s interaction with amyloid-beta peptides implicates it in Alzheimer’s disease by mitigating oxidative stress damage. Through these conditions its role as a protector against oxidative stress remains pivotal.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions (PubMed : 17182577, PubMed : 19032234, PubMed : 2176490). Plays a role in the reversible S-nitrosylation of cysteine residues in target proteins, and thereby contributes to the response to intracellular nitric oxide. Nitrosylates the active site Cys of CASP3 in response to nitric oxide (NO), and thereby inhibits caspase-3 activity (PubMed : 16408020, PubMed : 17606900). Induces the FOS/JUN AP-1 DNA-binding activity in ionizing radiation (IR) cells through its oxidation/reduction status and stimulates AP-1 transcriptional activity (PubMed : 11118054, PubMed : 9108029).. ADF augments the expression of the interleukin-2 receptor TAC (IL2R/P55).

See full target information TXN

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Annals of translational medicine 9:1474 PubMed34734026

2021

Induction of osteoclast formation by LOX mutant (LOXG473A) through regulation of autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Zhang,Chenglin Luo,Wenjin Xiao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914)

Western blot - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914)

OI-RD Scanning - Anti-Thioredoxin / TRX antibody [EPR6111] - BSA and Azide free (AB238914)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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