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AB129485

Anti-THOC2/Tho2 antibody

5

(2 Reviews)

|

(4 Publications)

Rabbit Polyclonal THOC2/Tho2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human THOC2 aa 1500 to C-terminus.

View Alternative Names

CXorf3, THOC2, THO complex subunit 2, Tho2, hTREX120

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THOC2/Tho2 antibody (AB129485)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THOC2/Tho2 antibody (AB129485)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling THOC2/Tho2 with ab129485 at 1/1000 (1μg/mg). Detection : DAB.

Immunoprecipitation - Anti-THOC2/Tho2 antibody (AB129485)
  • IP

Unknown

Immunoprecipitation - Anti-THOC2/Tho2 antibody (AB129485)

Lane 1 : ab129485 at 1 μg/ml staining THOC2/Tho2 in HeLa whole cell lysate immunoprecipitated using ab129485 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : control IgG.
Detection : Chemiluminescence
Exposure time : 1 second

All lanes:

Immunoprecipitation - Anti-THOC2/Tho2 antibody (ab129485)

Predicted band size: 183 kDa

false

Western blot - Anti-THOC2/Tho2 antibody (AB129485)
  • WB

Unknown

Western blot - Anti-THOC2/Tho2 antibody (AB129485)

All lanes:

Western blot - Anti-THOC2/Tho2 antibody (ab129485) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 183 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human THOC2 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8NI27

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Secondary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THOC2 also known as Tho2 is part of the THO complex and is an important player in mRNA processing and transport. This protein has an estimated mass of around 200 kDa and is mainly expressed in the nucleus of eukaryotic cells. THOC2 plays a significant role in RNA metabolism binding to nascent mRNA and helping with the integration of several other factors needed for RNA stability and transport to the cytoplasm.
Biological function summary

THOC2 is necessary for efficient mRNA export and is part of the larger transcription export (TREX) complex which involves several proteins including THOC1 THOC5 and UAP56. This complex couples transcription with mRNA processing contributing to the maturation and export of mRNA transcripts. THOC2 ensures the proper processing and exportation of mRNA preventing the accumulation of ribonucleoprotein complexes in the nucleus.

Pathways

THOC2 integrates into the process of gene expression where it links transcription with mRNA export. It participates in pathways related to RNA binding and export. During this THOC2 interacts with proteins such as ALYREF and SR proteins which further support the processes by facilitating efficient mRNA transport and splicing. Additionally the presence of THOC2 in the THO complex is essential for the stability of the complex influencing the overall gene expression regulation.

THOC2 mutations have associations with intellectual disabilities and other neurological issues. Mutations in the THOC2 gene can disrupt its normal function in RNA processing leading to deficits in brain development and function. Furthermore THOC2's connection with other THO complex proteins involves interactions implicated in these disorders. For example disruptions that affect the stability or function of other TREX components like UAP56 can amplify disease phenotypes by disturbing mRNA transport and processing.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). Required for efficient export of polyadenylated RNA and spliced mRNA (PubMed : 23222130). The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B; in the complex THOC2 is the only component that directly interacts with DDX39B (PubMed : 33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). Required for NXF1 localization to the nuclear rim (PubMed : 22893130). THOC2 (and probably the THO complex) is involved in releasing mRNA from nuclear speckle domains.. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.
See full target information THOC2
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 14:2170-2182 PubMed36438699

2022

MiR-30e-3p inhibits gastric cancer development by negatively regulating THO complex 2 and PI3K/AKT/mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Jing Gu,Ya-Jun Li,Fang Wang,Ting Ye

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e2102658 PubMed34708581

2021

THOC2 and THOC5 Regulate Stemness and Radioresistance in Triple-Negative Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xupeng Bai,Jie Ni,Julia Beretov,Shanping Wang,Xingli Dong,Peter Graham,Yong Li

Cancers 13: PubMed34572914

2021

HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Barreiro-Alonso,Mónica Lamas-Maceiras,Lidia Lorenzo-Catoira,Mercedes Pardo,Lu Yu,Jyoti S Choudhary,M Esperanza Cerdán

Nucleic acids research 46:9484-9495 PubMed30124983

2018

SPOP promotes transcriptional expression of DNA repair and replication factors to prevent replication stress and genomic instability.

Applications

Unspecified application

Species

Unspecified reactive species

Kim Hjorth-Jensen,Apolinar Maya-Mendoza,Nanna Dalgaard,Jón O Sigurðsson,Jiri Bartek,Diego Iglesias-Gato,Jesper V Olsen,Amilcar Flores-Morales
View all publications
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Product promise

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