Rabbit Polyclonal THOC2/Tho2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human THOC2 aa 1500 to C-terminus.
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Publications
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- World journal of gastrointestinal oncology 14:2170-21822022MiR-30e-3p inhibits gastric cancer development by negatively regulating THO complex 2 and PI3K/AKT/mTOR signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Jing Gu,Ya-Jun Li,Fang Wang,Ting YePubMed 36438699
- Cancers 13:2021HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAida Barreiro-Alonso,Mónica Lamas-Maceiras,Lidia Lorenzo-Catoira,Mercedes Pardo,Lu Yu,Jyoti S Choudhary,M Esperanza CerdánPubMed 34572914
- Nucleic acids research 46:9484-94952018SPOP promotes transcriptional expression of DNA repair and replication factors to prevent replication stress and genomic instability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKim Hjorth-Jensen,Apolinar Maya-Mendoza,Nanna Dalgaard,Jón O Sigurðsson,Jiri Bartek,Diego Iglesias-Gato,Jesper V Olsen,Amilcar Flores-MoralesPubMed 30124983
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human THOC2 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NI27
Reactivity data
Select an application| IHC-P | IP | WB | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Elephant | Predicted | Predicted | Predicted |
| Gorilla | Predicted | Predicted | Predicted |
| Horse | Predicted | Predicted | Predicted |
| Orangutan | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan, Elephant | Dilution info - | Notes - |
Target data
Function
Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for efficient export of polyadenylated RNA and spliced mRNA (PubMed:23222130). The THOC1-THOC2-THOC3 core complex alone is sufficient to bind export factor NXF1-NXT1 and promote ATPase activity of DDX39B; in the complex THOC2 is the only component that directly interacts with DDX39B (PubMed:33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed:15833825, PubMed:15998806, PubMed:17190602). Required for NXF1 localization to the nuclear rim (PubMed:22893130). THOC2 (and probably the THO complex) is involved in releasing mRNA from nuclear speckle domains. Plays a role for proper neuronal development. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.
Alternative names
CXorf3, THOC2, THO complex subunit 2, Tho2, hTREX120
Recommended products
Rabbit Polyclonal THOC2/Tho2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human THOC2 aa 1500 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human THOC2 aa 1500 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q8NI27
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
THOC2 also known as Tho2 is part of the THO complex and is an important player in mRNA processing and transport. This protein has an estimated mass of around 200 kDa and is mainly expressed in the nucleus of eukaryotic cells. THOC2 plays a significant role in RNA metabolism binding to nascent mRNA and helping with the integration of several other factors needed for RNA stability and transport to the cytoplasm.
- Biological function summary
THOC2 is necessary for efficient mRNA export and is part of the larger transcription export (TREX) complex which involves several proteins including THOC1 THOC5 and UAP56. This complex couples transcription with mRNA processing contributing to the maturation and export of mRNA transcripts. THOC2 ensures the proper processing and exportation of mRNA preventing the accumulation of ribonucleoprotein complexes in the nucleus.
- Pathways
THOC2 integrates into the process of gene expression where it links transcription with mRNA export. It participates in pathways related to RNA binding and export. During this THOC2 interacts with proteins such as ALYREF and SR proteins which further support the processes by facilitating efficient mRNA transport and splicing. Additionally the presence of THOC2 in the THO complex is essential for the stability of the complex influencing the overall gene expression regulation.
- Associated diseases and disorders
THOC2 mutations have associations with intellectual disabilities and other neurological issues. Mutations in the THOC2 gene can disrupt its normal function in RNA processing leading to deficits in brain development and function. Furthermore THOC2's connection with other THO complex proteins involves interactions implicated in these disorders. For example disruptions that affect the stability or function of other TREX components like UAP56 can amplify disease phenotypes by disturbing mRNA transport and processing.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-THOC2/Tho2 antibody (ab129485)
All lanes: Western blot - Anti-THOC2/Tho2 antibody (ab129485) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: 293T whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 183 kDa
Exposure time: 10s
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-THOC2/Tho2 antibody (ab129485)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling THOC2/Tho2 with ab129485 at 1/1000 (1μg/mg). Detection: DAB.
Immunoprecipitation - Anti-THOC2/Tho2 antibody (ab129485)
Lane 1: ab129485 at 1 μg/ml staining THOC2/Tho2 in HeLa whole cell lysate immunoprecipitated using ab129485 at 6 μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Detection: Chemiluminescence
Exposure time: 1 secondAll lanes: Immunoprecipitation - Anti-THOC2/Tho2 antibody (ab129485)
Predicted band size: 183 kDa
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