AB86070

Anti-THOC5 antibody

Name: Anti-THOC5 antibody (ab86070) | Abcam
Brand: Abcam Limited
SKU: AB86070

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(4 Publications)

Rabbit Polyclonal THOC5 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human THOC5 aa 300-350.

View Alternative Names

C22orf19, KIAA0983, THOC5, THO complex subunit 5, Functional spliceosome-associated protein 79, NF2/meningioma region protein pK1.3, Placental protein 39.2, hTREX90, fSAP79, PP39.2

2 Images

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Immunoprecipitation - Anti-THOC5 antibody (AB86070)

Detection of THOC5 by Western blot of Immunprecipitate.
ab86070, at 0.4 µg/ml, staining THOC5 in HeLa whole cell lysate immunoprecipitated using ab86070 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-THOC5 antibody (ab86070)

Predicted band size: 79 kDa

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Unknown

Western blot - Anti-THOC5 antibody (AB86070)

All lanes:

Western blot - Anti-THOC5 antibody (ab86070) at 0.04 µg/mL

Lane 1:

Hela whole cell lysate at 50 µg

Lane 2:

Hela whole cell lysate at 15 µg

Lane 3:

Hela whole cell lysate at 5 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

Exposure time: 90s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human THOC5 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q13769

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THOC5 also known as Fms-interacting protein 1 (FIP1L1) or HPR1 is a component of the transcription/export (TREX) complex. With a mass of around 100 kDa THOC5 operates at the intersection of transcription and mRNA processing. This protein localizes mainly in the nucleus where it executes its role in RNA metabolism. THOC5 is necessary for transporting newly synthesized mRNA from the nucleus to the cytoplasm and is constitutively expressed in various tissues including liver and testis.

Biological function summary

This THOC5 protein plays an important role in mRNA processing and export. As part of the TREX complex it assists in coupling transcription with mRNA processing and subsequent export. Through its orchestrated activity THOC5 facilitates the efficient flow of genetic information from DNA to protein synthesis machinery. THOC5 influences the stability and quality of mRNA transcripts by directly binding to certain RNA sequences.

Pathways

One can find THOC5 participating in the mRNA splicing and transport pathways. This protein's role is integrally linked with the DNA-directed RNA polymerase II contributing to the processes that manage RNA synthesis and modification. Additionally THOC5 interacts with other components of the TREX complex such as THOC2 and THOC7 to maintain proper RNA metabolism and cellular function.

THOC5 has relevance to cancer. Some studies have found dysregulation of THOC5 in various malignancies including leukemia and liver cancer. Its association with the FUS protein suggests a link to neurodegenerative conditions since FUS mutations show involvement in amyotrophic lateral sclerosis (ALS). Imbalances in THOC5 levels can cause aberrant mRNA processing contributing to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). Plays a key structural role in the oligomerization of the THO-DDX39B complex (PubMed : 33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability (PubMed : 18974867). Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes (PubMed : 23685434).. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.

See full target information THOC5

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:31605 PubMed39738217

2024

Prader-Willi syndrome protein necdin regulates the nucleocytoplasmic distribution and dopaminergic neuron development.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Yichun Zhang,Ying Hu,Xiangrong Tang,Zishan Gong,Ren-Bin Lu,Jia-da Li

Cancers 13: PubMed34572914

2021

HMGB1 Protein Interactions in Prostate and Ovary Cancer Models Reveal Links to RNA Processing and Ribosome Biogenesis through NuRD, THOC and Septin Complexes.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Barreiro-Alonso,Mónica Lamas-Maceiras,Lidia Lorenzo-Catoira,Mercedes Pardo,Lu Yu,Jyoti S Choudhary,M Esperanza Cerdán

PloS one 13:e0194887 PubMed29590189

2018

Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system.

Applications

Unspecified application

Species

Unspecified reactive species

Roman J Szczesny,Katarzyna Kowalska,Kamila Klosowska-Kosicka,Aleksander Chlebowski,Ewelina P Owczarek,Zbigniew Warkocki,Tomasz M Kulinski,Dorota Adamska,Kamila Affek,Agata Jedroszkowiak,Anna V Kotrys,Rafal Tomecki,Pawel S Krawczyk,Lukasz S Borowski,Andrzej Dziembowski

Nature communications 6:6720 PubMed25851487

2015

RECQ5-dependent SUMOylation of DNA topoisomerase I prevents transcription-associated genome instability.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Subhash Pokharel,Jiin-Tarng Wang,Xiaohua Xu,Yilun Liu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-THOC5 antibody

Immunoprecipitation - Anti-THOC5 antibody (AB86070)

Western blot - Anti-THOC5 antibody (AB86070)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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