AB284812

Anti-THOC5 antibody [EP6904] - BSA and Azide free

RabMAb
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Rabbit Monoclonal THOC5 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Rat, Human samples.

View Alternative Names

C22orf19, KIAA0983, THOC5, THO complex subunit 5, Functional spliceosome-associated protein 79, NF2/meningioma region protein pK1.3, Placental protein 39.2, hTREX90, fSAP79, PP39.2

2 Images

Flow Cytometry (Intracellular) - Anti-THOC5 antibody [EP6904] - BSA and Azide free (AB284812)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-THOC5 antibody [EP6904] - BSA and Azide free (AB284812)

This data was developed using ab137051, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293T cells labelling THOC5 with ab137051 at dilution 1/10 (pink) or a Rabbit IgG negative control (green).

Unknown

Western blot - Anti-THOC5 antibody [EP6904] - BSA and Azide free (AB284812)

This data was developed using ab137051, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-THOC5 antibody [EP6904] - BSA and Azide free (ab284812) at 1/1000 dilution

Lane 1:

293T cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

PC-12 at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 79 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP6904

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab284812 is the carrier-free version of ab137051

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Constituents: 100% PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THOC5 also known as Fms-interacting protein 1 (FIP1L1) or HPR1 is a component of the transcription/export (TREX) complex. With a mass of around 100 kDa THOC5 operates at the intersection of transcription and mRNA processing. This protein localizes mainly in the nucleus where it executes its role in RNA metabolism. THOC5 is necessary for transporting newly synthesized mRNA from the nucleus to the cytoplasm and is constitutively expressed in various tissues including liver and testis.

Biological function summary

This THOC5 protein plays an important role in mRNA processing and export. As part of the TREX complex it assists in coupling transcription with mRNA processing and subsequent export. Through its orchestrated activity THOC5 facilitates the efficient flow of genetic information from DNA to protein synthesis machinery. THOC5 influences the stability and quality of mRNA transcripts by directly binding to certain RNA sequences.

Pathways

One can find THOC5 participating in the mRNA splicing and transport pathways. This protein's role is integrally linked with the DNA-directed RNA polymerase II contributing to the processes that manage RNA synthesis and modification. Additionally THOC5 interacts with other components of the TREX complex such as THOC2 and THOC7 to maintain proper RNA metabolism and cellular function.

THOC5 has relevance to cancer. Some studies have found dysregulation of THOC5 in various malignancies including leukemia and liver cancer. Its association with the FUS protein suggests a link to neurodegenerative conditions since FUS mutations show involvement in amyotrophic lateral sclerosis (ALS). Imbalances in THOC5 levels can cause aberrant mRNA processing contributing to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). Plays a key structural role in the oligomerization of the THO-DDX39B complex (PubMed : 33191911). TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex (EJC) and is recruited in a splicing- and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP/NXF1 pathway (PubMed : 15833825, PubMed : 15998806, PubMed : 17190602). THOC5 in conjunction with ALYREF/THOC4 functions in NXF1-NXT1 mediated nuclear export of HSP70 mRNA; both proteins enhance the RNA binding activity of NXF1 and are required for NXF1 localization to the nuclear rim. Involved in transcription elongation and genome stability (PubMed : 18974867). Involved in alternative polyadenylation site choice by recruiting CPSF6 to 5' region of target genes; probably mediates association of the TREX and CFIm complexes (PubMed : 23685434).. Regulates the expression of myeloid transcription factors CEBPA, CEBPB and GAB2 by enhancing the levels of phosphatidylinositol 3,4,5-trisphosphate. May be involved in the differentiation of granulocytes and adipocytes. Essential for hematopoietic primitive cell survival and plays an integral role in monocytic development.. (Microbial infection) The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus (KSHV) intronless mRNAs and infectious virus production.

See full target information THOC5

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-THOC5 antibody [EP6904] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-THOC5 antibody [EP6904] - BSA and Azide free (AB284812)

Western blot - Anti-THOC5 antibody [EP6904] - BSA and Azide free (AB284812)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise