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AB270731

Anti-Thomsen-Friedenreich Antigen antibody [A78-G/A7]

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Mouse Monoclonal Thomsen-Friedenreich Antigen antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Cell preparation containing Thomsen-Friedenreich Antigen protein.

View Alternative Names

CD176, TF antigen

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [A78-G/A7] (AB270731)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [A78-G/A7] (AB270731)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Thomsen-Friedenreich Antigen using ab270731 at 2 μg/ml in immunohistochemical analysis.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

A78-G/A7

Isotype

IgM

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Recognizes a disaccharide epitope, Gal 1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF , including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin.

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Secondary Antibodies

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD44 antibody [EPR18668] (AB189524)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification notes
Purified from Bioreactor Concentrate.
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Thomsen-Friedenreich antigen (TF antigen) also known as CD176 is a cryptic carbohydrate antigen exposed on the surface of cells particularly after malignant transformation. It has a molecular mass that varies depending on the glycoprotein carrier. TF antigen often exists in an inactive form within normal epithelial and endothelial cells masked by sialic acid and becomes exposed in cancerous and certain pathological conditions. Its structural core consists of a galactose beta 1-3 N-acetylgalactosamine configuration.
Biological function summary

The TF antigen plays roles in cell adhesion signal transduction and as a marker of cancer progression. It is not part of a larger protein complex but serves a significant role by participating in cellular recognition and immune response evasion. Researchers identified it as a pan-carcinoma antigen often used as a target for cancer immunotherapy due to its expression in many tumor tissues such as breast and colorectal cancers.

Pathways

TF antigen is involved in processes related to cell adhesion and immune evasion. These interactions often include pathways like the PI3K/AKT signaling pathway which contribute to cell survival and proliferation and involve proteins such as integrins and lectins. The alteration in pathways due to TF expression changes helps cancer cells migrate and grow making it an important target for analyzing tumor behavior.

TF antigen closely associates with cancer specifically aiding in tumorigenesis and metastasis in breast and colorectal cancers. Its expression correlates with proteins like MUC1 and MUC2 which are mucins often overexpressed and modified in these cancers. The exposure of TF antigen on these mucins can be a marker for malignancy and the interaction further disrupts normal cell function contributing to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Thomsen-Friedenreich Antigen
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Product promise

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