AB17785

Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted

Name: Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (ab17785) | Abcam
Brand: Abcam Limited
SKU: AB17785
Price: 535 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Mouse Monoclonal antibody. Suitable for IHC-P and reacts with Human, Rat, Mouse samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing Thomsen-Friedenreich Antigen protein.

View Alternative Names

CD176, TF antigen

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (AB17785)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (AB17785)

Human colon adenocarcinoma stained with ab17785 (prediluted)

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM320

Isotype

IgM

Light chain type

kappa

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Epitope

Galß1-3GalNAc

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purity

IgM fraction

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Thomsen-Friedenreich antigen (TF antigen) also known as CD176 is a cryptic carbohydrate antigen exposed on the surface of cells particularly after malignant transformation. It has a molecular mass that varies depending on the glycoprotein carrier. TF antigen often exists in an inactive form within normal epithelial and endothelial cells masked by sialic acid and becomes exposed in cancerous and certain pathological conditions. Its structural core consists of a galactose beta 1-3 N-acetylgalactosamine configuration.

Biological function summary

The TF antigen plays roles in cell adhesion signal transduction and as a marker of cancer progression. It is not part of a larger protein complex but serves a significant role by participating in cellular recognition and immune response evasion. Researchers identified it as a pan-carcinoma antigen often used as a target for cancer immunotherapy due to its expression in many tumor tissues such as breast and colorectal cancers.

Pathways

TF antigen is involved in processes related to cell adhesion and immune evasion. These interactions often include pathways like the PI3K/AKT signaling pathway which contribute to cell survival and proliferation and involve proteins such as integrins and lectins. The alteration in pathways due to TF expression changes helps cancer cells migrate and grow making it an important target for analyzing tumor behavior.

TF antigen closely associates with cancer specifically aiding in tumorigenesis and metastasis in breast and colorectal cancers. Its expression correlates with proteins like MUC1 and MUC2 which are mucins often overexpressed and modified in these cancers. The exposure of TF antigen on these mucins can be a marker for malignancy and the interaction further disrupts normal cell function contributing to disease progression.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Thomsen-Friedenreich Antigen

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biochemistry and biophysics reports 5:313-327 PubMed28955838

2016

Mangiferin attenuates oxidative stress induced renal cell damage through activation of PI3K induced Akt and Nrf-2 mediated signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Sukanya Saha,Pritam Sadhukhan,Krishnendu Sinha,Namrata Agarwal,Parames C Sil

PloS one 9:e89026 PubMed24586486

2014

Amelioration of aspirin induced oxidative impairment and apoptotic cell death by a novel antioxidant protein molecule isolated from the herb Phyllanthus niruri.

Applications

Unspecified application

Species

Mouse

Sudip Bhattacharyya,Shatadal Ghosh,Parames C Sil

International journal of cancer 132:2107-17 PubMed23055136

2012

Multifunctional nanobeacon for imaging Thomsen-Friedenreich antigen-associated colorectal cancer.

Applications

Unspecified application

Species

Human

Hironori Kumagai,Wellington Pham,Makoto Kataoka,Ken-Ichiro Hiwatari,James McBride,Kevin J Wilson,Hiroyuki Tachikawa,Ryoji Kimura,Kunio Nakamura,Eric H Liu,John C Gore,Shinji Sakuma

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thomsen-Friedenreich Antigen antibody [SPM320], prediluted (AB17785)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Epitope

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise