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Rabbit Polyclonal Thrombin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 17 publications.


Images

Western blot - Anti-Thrombin antibody (AB92621), expandable thumbnail
  • Western blot - Anti-Thrombin antibody (AB92621), expandable thumbnail
  • Western blot - Anti-Thrombin antibody (AB92621), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Tested
Macaque monkey
Predicted
Orangutan
Predicted
Pig
Predicted

Tested
Tested

Species

Mouse

Dilution info

1 µg/mL

Notes

-

Species

Rat

Dilution info

1 µg/mL

Notes

-

Species

Human

Dilution info

1 µg/mL

Notes

-

Predicted
Predicted

Species

Pig, Macaque monkey, Orangutan

Dilution info

-

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.

Alternative names

Prothrombin, Coagulation factor II, F2

Recommended products

Rabbit Polyclonal Thrombin antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 17 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Thrombin also known as Factor II or thrombin protein is a 36 kDa serine protease essential for blood coagulation. It is produced in the liver as prothrombin and activated in the coagulation cascade. Its expression occurs extensively in the liver where it plays an important role in converting soluble fibrinogen into insoluble fibrin forming blood clots. Thrombin also activates various other coagulation factors including Factors V VIII and XI amplifying the coagulation response. Biotinylated thrombin variations help in detection and research applications.

Biological function summary

Thrombin modulates several physiological processes beyond clot formation. It serves as a signaling molecule interacting with protease-activated receptors (PARs) to influence cell functions including proliferation migration and apoptosis. Thrombin forms part of the prothrombinase complex comprised of prothrombin activated Factor X (Xa) and Factor V on phospholipid surfaces. This complex is critical for thrombin generation during the clotting cascade. Thrombin's activity extends to involvement in wound healing and inflammation regulation.

Pathways

Thrombin significantly participates in the coagulation and fibrinolytic pathways. It not only converts fibrinogen to fibrin in the coagulation pathway but also activates inhibitors like antithrombin which regulates thrombin and other protease activities. Thrombin's interaction with fibrinolysis where tPA and plasminogen are substrates integrates clot disintegration processes. Thrombin's connectivity with proteins such as Factor VII Protein C and antithrombin outlines its diverse role in maintaining hemostatic balance.

Associated diseases and disorders

Thrombin associates with conditions such as venous thromboembolism and disseminated intravascular coagulation (DIC). Dysregulation of thrombin leads to excessive clotting contributing to thromboembolic diseases. In DIC uncontrolled thrombin activity results in systemic coagulation consuming clotting factors and increasing bleeding risk. Its function alongside proteins like Factor V Leiden and antithrombin deficiencies aggravate thrombotic conditions. Research into thrombin inhibitors aids in developing therapeutic strategies for these disorders.

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3 product images

  • Western blot - Anti-Thrombin antibody (ab92621), expandable thumbnail

    Western blot - Anti-Thrombin antibody (ab92621)

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab92621 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ECL Substrate Kit (High Sensitivity) ab133406.

    All lanes: Western blot - Anti-Thrombin antibody (ab92621) at 1 µg/mL

    Lane 1: Liver (Human) Tissue Lysate at 10 µg

    Lane 2: Liver (Mouse) Tissue Lysate at 10 µg

    Lane 3: Liver (Rat) Tissue Lysate at 10 µg

    Lane 4: Plasma (Human) Total Protein Lysate at 10 µg

    Lane 5: Plasma (Mouse) Total Protein Lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 70 kDa

    Observed band size: 100 kDa, 60 kDa, 78 kDa

    Exposure time: 5s

  • Western blot - Anti-Thrombin antibody (ab92621), expandable thumbnail

    Western blot - Anti-Thrombin antibody (ab92621)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: Brain

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:17637839 and PMID:21131592).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Actual observed bands 33-72kDa.

    Exposure times: left panel: 10 seconds; right panel: 147 seconds.

    All lanes: Western blot - Anti-Prothrombin antibody [RM1252] (Anti-Prothrombin antibody [RM1252] ab322113) at 1/1000 dilution

    Lane 1: Mouse plasma lysate at 10 µg

    Lane 2: Mouse liver tissue lysate at 20 µg

    Lane 3: Mouse brain tissue lysate at 20 µg

    Lane 4: Mouse heart tissue lysate at 20 µg

    Lane 5: Mouse kidney tissue lysate at 20 µg

    Lane 6: Rat plasma lysate at 10 µg

    Lane 7: Rat liver tissue lysate at 20 µg

    Lane 8: Rat brain tissue lysate at 20 µg

    Lane 9: Rat heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 33-72 kDa, 36 kDa

  • Western blot - Anti-Thrombin antibody (ab92621), expandable thumbnail

    Western blot - Anti-Thrombin antibody (ab92621)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HeLa (PMID: 1463733).

    Low expression: cerebellum.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID:17637839 and PMID:21131592).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Actual observed bands 33-72kDa.

    All lanes: Western blot - Anti-Prothrombin antibody [RM1252] (Anti-Prothrombin antibody [RM1252] ab322113) at 1/1000 dilution

    Lane 1: Human plasma lysate at 4 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Lane 3: Human cerebellum tissue lysate at 20 µg

    Lane 4: Human heart tissue lysate at 20 µg

    Lane 5: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 6: Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 7: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 33-72 kDa, 36 kDa

    Exposure time: 180s

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