AB314480

Anti-Thrombin antibody [EPR25304-12]

20ul selling size
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Thrombin antibody. Suitable for WB and reacts with Human, Mouse, Rat samples.

View Alternative Names

Prothrombin, Coagulation factor II, F2

4 Images

Supplier Data

Western blot - Anti-Thrombin antibody [EPR25304-12] (AB314480)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 31324782, 21527525, 22345489). In Western blot, Anti-Bovine Serum Albumin antibody [EPR12774] (ab192603) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Thrombin antibody [EPR25304-12] (ab314480) at 1/1000 dilution

All lanes:

Human plasma lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 50 kDa,60 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-Thrombin antibody [EPR25304-12] (AB314480)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Low expression : SW480 (PMID : 31324782). ab314480 demonstrated significant selectivity for a-thrombin over prothrombin. This data is displayed compared to ab208589 which demonstrates enhanced selectivity for prothrombin over a-thrombin. The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 31324782, 21527525, 22345489). Lanes 1-3 are applied with Anti-Thrombin antibody [EPR25304] (ab314480) at 1/1000 dilution and lanes 4-6 are applied with Anti-Prothrombin antibody [EPR20159] (ab208589) at 1/1000 dilution. In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Lanes 1 - 3:

Western blot - Anti-Thrombin antibody [EPR25304-12] (ab314480) at 1/1000 dilution

Lanes 4 - 6:

Western blot - Anti-Prothrombin antibody [EPR20159] (<a href='/en-us/products/primary-antibodies/prothrombin-antibody-epr20159-ab208589'>ab208589</a>) at 1/1000 dilution

Lanes 1 and 4:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 2 and 5:

Caco-2 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 3 and 6:

SW480 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 38 kDa,37 kDa

false

Exposure time: 59s

Supplier Data

Western blot - Anti-Thrombin antibody [EPR25304-12] (AB314480)

All lanes:

Western blot - Anti-Thrombin antibody [EPR25304-12] (ab314480) at 1/1000 dilution

Lane 1:

Mouse plasma lysate at 20 µg

Lane 2:

Mouse serum lysate at 20 µg

Lane 3:

Rat serum lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa

true

Exposure time: 180s

Supplier Data

Western blot - Anti-Thrombin antibody [EPR25304-12] (AB314480)

Blocking and diluting buffer and concentration : 5% NFDM/TBST The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 31324782, 21527525, 22345489). In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Thrombin antibody [EPR25304-12] (ab314480) at 1/1000 dilution

All lanes:

NMuLi (mouse liver epithelial) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 38 kDa,37 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25304-12

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombin also known as Factor II or thrombin protein is a 36 kDa serine protease essential for blood coagulation. It is produced in the liver as prothrombin and activated in the coagulation cascade. Its expression occurs extensively in the liver where it plays an important role in converting soluble fibrinogen into insoluble fibrin forming blood clots. Thrombin also activates various other coagulation factors including Factors V VIII and XI amplifying the coagulation response. Biotinylated thrombin variations help in detection and research applications.

Biological function summary

Thrombin modulates several physiological processes beyond clot formation. It serves as a signaling molecule interacting with protease-activated receptors (PARs) to influence cell functions including proliferation migration and apoptosis. Thrombin forms part of the prothrombinase complex comprised of prothrombin activated Factor X (Xa) and Factor V on phospholipid surfaces. This complex is critical for thrombin generation during the clotting cascade. Thrombin's activity extends to involvement in wound healing and inflammation regulation.

Pathways

Thrombin significantly participates in the coagulation and fibrinolytic pathways. It not only converts fibrinogen to fibrin in the coagulation pathway but also activates inhibitors like antithrombin which regulates thrombin and other protease activities. Thrombin's interaction with fibrinolysis where tPA and plasminogen are substrates integrates clot disintegration processes. Thrombin's connectivity with proteins such as Factor VII Protein C and antithrombin outlines its diverse role in maintaining hemostatic balance.

Thrombin associates with conditions such as venous thromboembolism and disseminated intravascular coagulation (DIC). Dysregulation of thrombin leads to excessive clotting contributing to thromboembolic diseases. In DIC uncontrolled thrombin activity results in systemic coagulation consuming clotting factors and increasing bleeding risk. Its function alongside proteins like Factor V Leiden and antithrombin deficiencies aggravate thrombotic conditions. Research into thrombin inhibitors aids in developing therapeutic strategies for these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Activates coagulation factor XI (F11); activation is promoted by the contact with negatively charged surfaces (PubMed : 2019570, PubMed : 21976677). Triggers the production of pro-inflammatory cytokines, such as MCP-1/CCL2 and IL8/CXCL8, in endothelial cells (PubMed : 30568593, PubMed : 9780208).

See full target information F2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thrombin antibody [EPR25304-12]

Western blot - Anti-Thrombin antibody [EPR25304-12] (AB314480)

Western blot - Anti-Thrombin antibody [EPR25304-12] (AB314480)

Western blot - Anti-Thrombin antibody [EPR25304-12] (AB314480)

Western blot - Anti-Thrombin antibody [EPR25304-12] (AB314480)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise