AB230010

Anti-Thrombomodulin antibody [EPR18217-209]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal Thrombomodulin antibody. Suitable for IP, Flow Cyt, WB, ICC/IF, IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 8 publications.

View Alternative Names

CD141, Thrombomodulin, TM, Fetomodulin, Thbd

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse lung (PMID : 23946288; PMID : 10231031) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunohistochemistry (Frozen sections) - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse embryo E14.5 (developing lung) tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution (green). Positive membrane staining in the developing lung in mouse E14.5 embryo (PMID : 28306049) is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Flow cytometric analysis of bEND.3 (mouse brain endothelioma cell line) cells labeling Thrombomodulin with ab230010 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

Gated on total viable cells.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Immunohistochemical analysis of paraffin-embedded mouse stomach tissue labeling Thrombomodulin with ab230010 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on endothelial cells of mouse stomach (PMID : 23946288; PMID : 10231031) is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) cells labeling Thrombomodulin with ab230010 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in bEND.3 cell line (PMID : 7622601; PMID : 8223719).

The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) at 1/200 dilution (red).

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Supplier Data

Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Thrombomodulin was immunoprecipitated from 0.35 mg of bEND.3 (mouse brain endothelioma cell line) whole cell lysate with ab230010 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230010 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : bEND.3 whole cell lysate 10 μg (Input).
Lane 2 : ab230010 IP in bEND.3 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab230010 in bEND.3 whole cell lysate.

Exposure time : 10 seconds.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Predicted band size: 60 kDa

Observed band size: 105 kDa

true

Supplier Data

Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Thrombomodulin was immunoprecipitated from 0.35 mg of mouse lung tissue lysate with ab230010 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab230010 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.

Lane 1 : Mouse lung tissue lysate 10 μg (Input).
Lane 2 : ab230010 IP in mouse lung tissue lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab230010 in mouse lung tissue lysate.

Exposure time : 10 seconds.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010)

Predicted band size: 60 kDa

Observed band size: 105 kDa,75 kDa

true

Supplier Data

Western blot - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Exposure times : Lane 1 : 1 minute; Lane 2 : 20 seconds; Lane 3 : 10 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.

All lanes:

Western blot - Anti-Thrombomodulin antibody [EPR18217-209] (ab230010) at 1/1000 dilution

Lane 1:

Mouse lung tissue lysate at 20 µg

Lane 2:

bEND.3 (mouse brain endothelioma cell line) whole cell lysate at 10 µg

Lane 3:

Mouse placenta tissue lysate at 10 µg

Secondary

Lane 1:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 2 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 60 kDa

Observed band size: 105 kDa,75 kDa

true

Carrier free

Anti-Thrombomodulin antibody [EPR18217-209] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18217-209

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

WB, IP, IHC-P, ICC/IF, IHC-Fr, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/500", "IHCFr-species-notes": "Perform heat mediated antigen retrieval by using sodium citrate buffer (10 mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.

Biological function summary

Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.

Pathways

Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.

Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis. Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway. Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (By similarity). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (PubMed : 38174561). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (By similarity).

See full target information Thbd

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:324 PubMed40597299

2025

Mesenchymal stromal cell secretome reduces lung injury and thrombo-inflammation induced by SARS-CoV-2 spike protein.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Pezzotta,Alessandra Bovio,Barbara Imberti,Monica Locatelli,Daniela Corna,Domenico Cerullo,Sara Gastoldi,Ariela Benigni,Giuseppe Remuzzi,Marina Morigi,Luca Perico

Discover oncology 16:1047 PubMed40493298

2025

TCF19 expression and significance analysis in breast cancer: integrated bioinformatics analysis and histological validation.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyang Yu,Jingjing Wei,Qinhao Chen,Yueyin Pan,Xiaojun Qian,Jing Pan,Yubei Sun

Nature communications 15:3976 PubMed38729948

2024

TFPI from erythroblasts drives heme production in central macrophages promoting erythropoiesis in polycythemia.

Applications

Unspecified application

Species

Unspecified reactive species

Jun-Kai Ma,Li-Da Su,Lin-Lin Feng,Jing-Lin Li,Li Pan,Qupei Danzeng,Yanwei Li,Tongyao Shang,Xiao-Lin Zhan,Si-Ying Chen,Shibo Ying,Jian-Rao Hu,Xue Qun Chen,Qi Zhang,Tingbo Liang,Xin-Jiang Lu

Cell death discovery 10:167 PubMed38589400

2024

Thrombomodulin reduces α-synuclein generation and ameliorates neuropathology in a mouse model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Yun Niu,Xi-Xiu Xie,Hou-Zhen Tuo,Cui-Ping Lv,Ya-Ru Huang,Jie Zhu,Shi-Yu Liang,Xiao-Yu Du,Cheng-Gang Yang,Sheng-Jie Hou,Xiao-Ying Sun,Ling-Jie Li,Fang Cui,Qi-Xin Huang,Ying-Bo Jia,Yu-Jiong Wang,Rui-Tian Liu

Physiology international 109:215-229 PubMed35895566

2022

Uridine alleviates LPS-induced ARDS and improves insulin sensitivity by decreasing oxidative stress and inflammatory processes.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Bin Li,Degang Zhang,Zhuo Wang,Ye Zhao,Qin Yu

Biomedicines 10: PubMed35740360

2022

Angiopoietin-2 Inhibition of Thrombomodulin-Mediated Anticoagulation-A Novel Mechanism That May Contribute to Hypercoagulation in Critically Ill COVID-19 Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Hultström,Karin Fromell,Anders Larsson,Barbro Persson,Bo Nilsson,Susan E Quaggin,Christer Betsholtz,Robert Frithiof,Miklos Lipcsey,Marie Jeansson

Legal medicine (Tokyo, Japan) 51:101897 PubMed33940278

2021

Immunohistochemical analysis of thrombomodulin expression in myocardial tissue from autopsy cases of ischemic heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Takeshi Kondo,Motonori Takahashi,Gentaro Yamasaki,Marie Sugimoto,Azumi Kuse,Mai Morichika,Kanako Nakagawa,Makoto Sakurada,Migiwa Asano,Yasuhiro Ueno

Cell reports 29:1832-1847.e8 PubMed31722201

2019

Single-Cell Transcriptomics Uncovers Zonation of Function in the Mesenchyme during Liver Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ross Dobie,John R Wilson-Kanamori,Beth E P Henderson,James R Smith,Kylie P Matchett,Jordan R Portman,Karolina Wallenborg,Simone Picelli,Anna Zagorska,Swetha V Pendem,Thomas E Hudson,Minnie M Wu,Grant R Budas,David G Breckenridge,Ewen M Harrison,Damian J Mole,Stephen J Wigmore,Prakash Ramachandran,Chris P Ponting,Sarah A Teichmann,John C Marioni,Neil C Henderson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thrombomodulin antibody [EPR18217-209]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Immunohistochemistry (Frozen sections) - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Flow Cytometry - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Immunocytochemistry/ Immunofluorescence - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Immunoprecipitation - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

Western blot - Anti-Thrombomodulin antibody [EPR18217-209] (AB230010)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise