Anti-Thrombomodulin antibody [EPR4051]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Unpurified ab109189 at 1/100 dilution staining Thrombomodulin in Human squamous cervical carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Immunohistochemical analysis of paraffin embedded normal Human lung tissue using unpurified ab109189 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using unpurified ab109189 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Immunohistochemical staining of paraffin embedded human lung with purified ab109189 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Immunofluorescence staining of A431 cells with purified ab109189 at a working dilution of 1/300 counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077) used at a dilution of 1/1000. ab7291 a mouse anti-tubulin antibody (1/1000) was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse 1/1000) shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1 purified ab109189 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2 ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Thrombomodulin with purified ab109189 at 1/150 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Unpurified ab109189 at 1/100 dilution staining Thombomodulin in A431 cells by Immunofluorescence.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using unpurified ab109189 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Unpurified ab109189 at 1/100 dilution staining Thrombomodulin in Human placenta tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

AbReview34845****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Unpurified ab109189 staining Thrombomodulin in Human artery tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Biotin-conjugated Goatanti-rabbit polyclonal (1/200) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview.

• IP

Unknown

Immunoprecipitation - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

ab109189 (purified) at 1/90 immunoprecipitating thrombomodulin in 10 μg human placenta whole cell lysate (Lanes 1 and 2, observed at 100 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration : 5% NFDM/TBST Dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-Thrombomodulin antibody [EPR4051] (ab109189)

Predicted band size: 60 kDa

false

• WB

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Western blot - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

All lanes:

Western blot - Anti-Thrombomodulin antibody [EPR4051] (ab109189) at 1/1000 dilution

Lane 1:

THP-1 cell lysate at 10 µg

Lane 2:

Human placenta lysate at 10 µg

Lane 3:

Human heart lysate at 10 µg

Predicted band size: 60 kDa

false

• WB

Lab

Western blot - Anti-Thrombomodulin antibody [EPR4051] (AB109189)

Blocking buffer : 5% NFDM/TBST
Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Thrombomodulin antibody [EPR4051] (ab109189) at 1/10000 dilution

All lanes:

human placenta lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 60 kDa

Observed band size: 100 kDa

false