Name: Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free
SKU: ab271880
Rating: 4 (2 reviews)

Rabbit Recombinant Monoclonal Thrombomodulin antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (AB271880), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

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Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P	ICC/IF	Flow Cyt (Intra)
Human	Tested	Expected	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Associated Products

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1 product for Alternative Version

Target data

See full target information THBD

Function

Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed:10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed:29323190, PubMed:33836597, PubMed:9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed:26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed:15841214).

Alternative names

CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin

Recommended products

Rabbit Recombinant Monoclonal Thrombomodulin antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR4051
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab271880 is the carrier-free version of Anti-Thrombomodulin antibody [EPR4051] ab109189.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.

Biological function summary

Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.

Pathways

Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.

Associated diseases and disorders

Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Immunohistochemical staining of paraffin embedded human lung with purified Anti-Thrombomodulin antibody [EPR4051] ab109189 at a working dilution of 1/1000. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
Immunocytochemistry/ Immunofluorescence - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Immunofluorescence staining of A431 cells with purified Anti-Thrombomodulin antibody [EPR4051] ab109189 at a working dilution of 1/300, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified Anti-Thrombomodulin antibody [EPR4051] ab109189 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at a dilution of 1/500. For negative control 2, Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at a dilution of 1/400.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
Immunoprecipitation - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Anti-Thrombomodulin antibody [EPR4051] ab109189 (purified) at 1/90 immunoprecipitating thrombomodulin in 10 μg human placenta whole cell lysate (Lanes 1 and 2, observed at 100 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
All lanes: Immunoprecipitation - Anti-Thrombomodulin antibody [EPR4051] (Anti-Thrombomodulin antibody [EPR4051] ab109189)
Predicted band size: 60 kDa
Flow Cytometry (Intracellular) - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Intracellular Flow Cytometry analysis of A431 (human epidermoid carcinoma) cells labeling Thrombomodulin with purified Anti-Thrombomodulin antibody [EPR4051] ab109189 at 1/150 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
Immunocytochemistry/ Immunofluorescence - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Unpurified Anti-Thrombomodulin antibody [EPR4051] ab109189, at 1/100 dilution, staining Thombomodulin in A431 cells by Immunofluorescence.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Unpurified Anti-Thrombomodulin antibody [EPR4051] ab109189, at 1/100 dilution, staining Thrombomodulin in Human placenta tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Unpurified Anti-Thrombomodulin antibody [EPR4051] ab109189, at 1/100 dilution, staining Thrombomodulin in Human squamous cervical carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using unpurified Anti-Thrombomodulin antibody [EPR4051] ab109189 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using unpurified Anti-Thrombomodulin antibody [EPR4051] ab109189 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
This image is courtesy of an anonymous customer review.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Unpurified Anti-Thrombomodulin antibody [EPR4051] ab109189 staining Thrombomodulin in Human artery tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 20% serum for 60 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. A Biotin-conjugated Goatanti-rabbit polyclonal (1/200) was used as the secondary antibody.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [EPR4051] - BSA and Azide free (ab271880)
Immunohistochemical analysis of paraffin embedded normal Human lung tissue using unpurified Anti-Thrombomodulin antibody [EPR4051] ab109189 showing +ve staining.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Thrombomodulin antibody [EPR4051] ab109189).