AB6980

Anti-Thrombomodulin antibody [PBS-01]

Name: Anti-Thrombomodulin antibody [PBS-01] (ab6980) | Abcam
Brand: Abcam Limited
SKU: AB6980
Price: 545 USD
Availability: InStock
Rating: 4 (7 reviews)

(7 Reviews)

(23 Publications)

Mouse Monoclonal Thrombomodulin antibody. Suitable for IHC-P, Flow Cyt, ELISA, WB and reacts with Human, Rabbit samples. Cited in 23 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human THBD.

View Alternative Names

CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin

2 Images

Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (AB6980)

Flow Cyt

Unknown

Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (AB6980)

Overlay histogram showing THP1 cells stained with ab6980 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6980, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (AB6980)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (AB6980)

ab6980 staining human normal lung endothelium. Staining is localized to the membrane.
Left panel : with primary antibody at 1 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

PBS-01

Isotype

IgG1

Carrier free

Reacts with

Human, Rabbit

Applications

Flow Cyt, WB, IHC-P, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human THBD.

P07204

Epitope

EGF domain 5 of thrombomodulin.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1 µg for 10^6 Cells", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 6.5 Constituents: 4% Sucrose, 0.41% Sodium acetate, 0.292% Sodium chloride, 0.1% Sorbitan monolaurate, ethoxylated

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.

Biological function summary

Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.

Pathways

Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.

Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed : 10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed : 29323190, PubMed : 33836597, PubMed : 9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed : 26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed : 15841214).

See full target information THBD

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Haematologica 110:414-424 PubMed39234869

2024

Impact of soluble thrombomodulin and activated protein C on dynamic hemostatic function in trauma: a focus on thrombin generation and clot lysis.

Applications

Unspecified application

Species

Unspecified reactive species

Nicola S Curry,Jeries Abu-Hanna,Gael B Morrow,Robin Choudhury,Michael Laffan

Genes 15: PubMed38790160

2024

The Immune Microenvironment Landscape of Pituitary NeuroEndocrine Tumors, a Transcriptomic Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Sandra Vela-Patiño,Ma Isabel Salazar,Keiko Taniguchi-Ponciano,Eduardo Vadillo,Erick Gomez-Apo,Aurea Escobar-España,Vadim Perez-Koldenkova,Laura Bonifaz,Cristina Aguilar-Flores,Daniel Marrero-Rodríguez,Moises Mercado

Nature 622:393-401 PubMed37821590

2023

Design and testing of a humanized porcine donor for xenotransplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Ranjith P Anand,Jacob V Layer,David Heja,Takayuki Hirose,Grace Lassiter,Daniel J Firl,Violette B Paragas,Adam Akkad,Sagar Chhangawala,Robert B Colvin,Russell J Ernst,Nicholas Esch,Kristen Getchell,Alexandra K Griffin,Xiaoyun Guo,Katherine C Hall,Paula Hamilton,Lokesh A Kalekar,Yinan Kan,Ahmad Karadagi,Feng Li,Susan C Low,Rudy Matheson,Claudia Nehring,Ryo Otsuka,Matthew Pandelakis,Robert A Policastro,Rebecca Pols,Luis Queiroz,Ivy A Rosales,William T Serkin,Kathryn Stiede,Toshihide Tomosugi,Yongqiang Xue,Gabriel E Zentner,David Angeles-Albores,J Chris Chao,Juliet N Crabtree,Sierra Harken,Nicole Hinkle,Tania Lemos,Mailin Li,Lorena Pantano,Denise Stevens,Omar D Subedar,Xiaoqing Tan,Shiyi Yin,Imran J Anwar,David Aufhauser,Saverio Capuano,Dixon B Kaufman,Stuart J Knechtle,Jean Kwun,Dhanansayan Shanmuganayagam,James F Markmann,George M Church,Mike Curtis,Tatsuo Kawai,Michele E Youd,Wenning Qin

Journal of oncology 2022:9775736 PubMed36276271

2022

A Machine Learning Workflow of Multiplexed Immunofluorescence Images to Interrogate Activator and Tolerogenic Profiles of Conventional Type 1 Dendritic Cells Infiltrating Melanomas of Disease-Free and Metastatic Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Saraí G De León Rodríguez,Paúl Hernández Herrera,Cristina Aguilar Flores,Vadim Pérez Koldenkova,Adán Guerrero,Alejandra Mantilla,Ezequiel M Fuentes-Pananá,Christopher Wood,Laura C Bonifaz

The American journal of pathology 191:1526-1536 PubMed34116023

2021

Recombinant Antithrombin Attenuates Acute Respiratory Distress Syndrome in Experimental Endotoxemia.

Applications

Unspecified application

Species

Unspecified reactive species

Haruka Okamoto,Isamu Muraki,Hideshi Okada,Hiroyuki Tomita,Kodai Suzuki,Chihiro Takada,Yugo Wakayama,Ayumi Kuroda,Hirotsugu Fukuda,Yuki Kawasaki,Ayane Nishio,Maho Matsuo,Yuto Tamaoki,Risa Inagawa,Shigeo Takashima,Toshiaki Taniguchi,Akio Suzuki,Keiko Suzuki,Nagisa Miyazaki,Yoshinori Kakino,Ryu Yasuda,Tetsuya Fukuta,Yuichiro Kitagawa,Takahito Miyake,Tomoaki Doi,Takahiro Yoshida,Shozo Yoshida,Shinji Ogura

The American journal of pathology 189:1526-1535 PubMed31108101

2019

Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia.

Applications

Unspecified application

Species

Unspecified reactive species

Kodai Suzuki,Hideshi Okada,Genzou Takemura,Chihiro Takada,Ayumi Kuroda,Hirohisa Yano,Ryogen Zaikokuji,Kentaro Morishita,Hiroyuki Tomita,Kazumasa Oda,Saori Matsuo,Akihiro Uchida,Tetsuya Fukuta,So Sampei,Nagisa Miyazaki,Tomonori Kawaguchi,Takatomo Watanabe,Takahiro Yoshida,Hiroaki Ushikoshi,Shozo Yoshida,Yoichi Maekawa,Shinji Ogura

Blood advances 2:2917-2921 PubMed30396911

2018

Persistence of endothelial thrombomodulin in a patient with infectious purpura fulminans treated with protein C concentrate.

Applications

Unspecified application

Species

Unspecified reactive species

Pavan K Bendapudi,Alissa Robbins,Nicole LeBoeuf,Olga Pozdnyakova,Ami Bhatt,Fujiko Duke,Ryan Sells,John McQuiston,Ben Humrighouse,Betty Rouaisnel,Meaghan Colling,Kathryn E Stephenson,Arturo Saavedra,Julie-Aurore Losman

The Journal of surgical research 229:28-40 PubMed29937002

2018

CRISPR/Cas and recombinase-based human-to-pig orthotopic gene exchange for xenotransplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael Miyashiro Nunes Dos Santos,Luiz Augusto Carneiro D'Albuquerque,Luz M Reyes,Jose L Estrada,Zheng-Yu Wang,Matthew Tector,A Joseph Tector

Clinical immunology (Orlando, Fla.) 191:27-33 PubMed29562205

2018

Thrombomodulin (TM) in tumor cell differentiation and periphery blood immune microenvironment in oral squamous cell carcinoma.

Applications

IHC-P

Species

Human

Jingjing Song,Da Ma,Xiangqi Liu,Yichen Chen,Juan Fang,Vivian Wai Yan Lui,Sijia Zhao,Juan Xia,Bin Cheng,Zhi Wang

APL bioengineering 2:016101 PubMed31069286

2017

Synthesis and targeting of gold-coated Lu-containing lanthanide phosphate nanoparticles-A potential theranostic agent for pulmonary metastatic disease.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Sobol,Logan Sutherlin,Edyta Cedrowska,Joshua Schorp,Cristina Rodríguez-Rodríguez,Vesna Sossi,Jimmy Lattimer,Douglas C Miller,Paul Pevsner,J David Robertson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Thrombomodulin antibody [PBS-01]

Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (AB6980)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (AB6980)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Epitope

Alternative Products

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Primary Antibodies

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Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise