Mouse Monoclonal Thrombomodulin antibody. Suitable for IHC-P, Flow Cyt, ELISA, WB and reacts with Human, Rabbit samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human THBD.
Images
![Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (AB6980), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-pbs-01-ab6980--flow-cytometry-img50179.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (AB6980)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (AB6980), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-pbs-01-ab6980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15578.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (AB6980)")
Publications
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- Nature 622:393-4012023Design and testing of a humanized porcine donor for xenotransplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRanjith P Anand,Jacob V Layer,David Heja,Takayuki Hirose,Grace Lassiter,Daniel J Firl,Violette B Paragas,Adam Akkad,Sagar Chhangawala,Robert B Colvin,Russell J Ernst,Nicholas Esch,Kristen Getchell,Alexandra K Griffin,Xiaoyun Guo,Katherine C Hall,Paula Hamilton,Lokesh A Kalekar,Yinan Kan,Ahmad Karadagi,Feng Li,Susan C Low,Rudy Matheson,Claudia Nehring,Ryo Otsuka,Matthew Pandelakis,Robert A Policastro,Rebecca Pols,Luis Queiroz,Ivy A Rosales,William T Serkin,Kathryn Stiede,Toshihide Tomosugi,Yongqiang Xue,Gabriel E Zentner,David Angeles-Albores,J Chris Chao,Juliet N Crabtree,Sierra Harken,Nicole Hinkle,Tania Lemos,Mailin Li,Lorena Pantano,Denise Stevens,Omar D Subedar,Xiaoqing Tan,Shiyi Yin,Imran J Anwar,David Aufhauser,Saverio Capuano,Dixon B Kaufman,Stuart J Knechtle,Jean Kwun,Dhanansayan Shanmuganayagam,James F Markmann,George M Church,Mike Curtis,Tatsuo Kawai,Michele E Youd,Wenning QinPubMed 37821590
- Journal of oncology 2022:97757362022A Machine Learning Workflow of Multiplexed Immunofluorescence Images to Interrogate Activator and Tolerogenic Profiles of Conventional Type 1 Dendritic Cells Infiltrating Melanomas of Disease-Free and Metastatic Patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSaraí G De León Rodríguez,Paúl Hernández Herrera,Cristina Aguilar Flores,Vadim Pérez Koldenkova,Adán Guerrero,Alejandra Mantilla,Ezequiel M Fuentes-Pananá,Christopher Wood,Laura C BonifazPubMed 36276271
- The American journal of pathology 191:1526-15362021Recombinant Antithrombin Attenuates Acute Respiratory Distress Syndrome in Experimental Endotoxemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaruka Okamoto,Isamu Muraki,Hideshi Okada,Hiroyuki Tomita,Kodai Suzuki,Chihiro Takada,Yugo Wakayama,Ayumi Kuroda,Hirotsugu Fukuda,Yuki Kawasaki,Ayane Nishio,Maho Matsuo,Yuto Tamaoki,Risa Inagawa,Shigeo Takashima,Toshiaki Taniguchi,Akio Suzuki,Keiko Suzuki,Nagisa Miyazaki,Yoshinori Kakino,Ryu Yasuda,Tetsuya Fukuta,Yuichiro Kitagawa,Takahito Miyake,Tomoaki Doi,Takahiro Yoshida,Shozo Yoshida,Shinji OguraPubMed 34116023
- The American journal of pathology 189:1526-15352019Neutrophil Elastase Damages the Pulmonary Endothelial Glycocalyx in Lipopolysaccharide-Induced Experimental Endotoxemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKodai Suzuki et. al.PubMed 31108101
- Blood advances 2:2917-29212018Persistence of endothelial thrombomodulin in a patient with infectious purpura fulminans treated with protein C concentrate.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPavan K Bendapudi,Alissa Robbins,Nicole LeBoeuf,Olga Pozdnyakova,Ami Bhatt,Fujiko Duke,Ryan Sells,John McQuiston,Ben Humrighouse,Betty Rouaisnel,Meaghan Colling,Kathryn E Stephenson,Arturo Saavedra,Julie-Aurore LosmanPubMed 30396911
- The Journal of surgical research 229:28-402018CRISPR/Cas and recombinase-based human-to-pig orthotopic gene exchange for xenotransplantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRafael Miyashiro Nunes Dos Santos et. al.PubMed 29937002
- Clinical immunology (Orlando, Fla.) 191:27-332018Thrombomodulin (TM) in tumor cell differentiation and periphery blood immune microenvironment in oral squamous cell carcinoma.Applications:IHC-PReactive species:HumanJingjing Song,Da Ma,Xiangqi Liu,Yichen Chen,Juan Fang,Vivian Wai Yan Lui,Sijia Zhao,Juan Xia,Bin Cheng,Zhi WangPubMed 29562205
- APL bioengineering 2:0161012017Synthesis and targeting of gold-coated Lu-containing lanthanide phosphate nanoparticles-A potential theranostic agent for pulmonary metastatic disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNicholas Sobol,Logan Sutherlin,Edyta Cedrowska,Joshua Schorp,Cristina Rodríguez-Rodríguez,Vesna Sossi,Jimmy Lattimer,Douglas C Miller,Paul Pevsner,J David RobertsonPubMed 31069286
- Molecular medicine reports 17:1651-16592017Protective effects of atorvastatin on cerebral vessel autoregulation in an experimental rabbit model of subarachnoid hemorrhage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun-Hui Chen,Ting Wu,Li-Kun Yang,Lei Chen,Jie Zhu,Pei-Pei Li,Xu Hu,Yu-Hai WangPubMed 29257200
- Molecular medicine reports 17:1724-17302017Effect of cigarette smoke extract and nicotine on the expression of thrombomodulin and endothelial protein C receptor in cultured human umbilical vein endothelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYujie Wei,Bin Lai,Huiliang Liu,Yi Li,Wang Zhen,Ling FuPubMed 29257196
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 6.5
Constituents: 4% Sucrose, 0.41% Sodium acetate, 0.292% Sodium chloride, 0.1% Sorbitan monolaurate, ethoxylated- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human THBD. Database link P07204
Abcam Recommends
Reactivity data
Select an application| IHC-P | Flow Cyt | ELISA | WB | |
|---|---|---|---|---|
| Human | Tested | Tested | Expected | Expected |
| Pig | Not recommended | Not recommended | Not recommended | Not recommended |
| Rabbit | Not recommended | Not recommended | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rabbit | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species Rabbit | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed:10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed:29323190, PubMed:33836597, PubMed:9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed:26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed:15841214).
Alternative names
CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin
Recommended products
Mouse Monoclonal Thrombomodulin antibody. Suitable for IHC-P, Flow Cyt, ELISA, WB and reacts with Human, Rabbit samples. Cited in 20 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human THBD.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 6.5
Constituents: 4% Sucrose, 0.41% Sodium acetate, 0.292% Sodium chloride, 0.1% Sorbitan monolaurate, ethoxylated- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human THBD. Database link P07204
- Clone number
- PBS-01
- Purification technique
- Affinity purification Protein G
- Epitope
- EGF domain 5 of thrombomodulin.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.
- Biological function summary
Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.
- Pathways
Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.
- Associated diseases and disorders
Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-pbs-01-ab6980--flow-cytometry-img50179.jpg "Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)")
Flow Cytometry - Anti-Thrombomodulin antibody [PBS-01] (ab6980)
Overlay histogram showing THP1 cells stained with ab6980 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab6980, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-pbs-01-ab6980--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img15578.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [PBS-01] (ab6980)
ab6980 staining human normal lung endothelium. Staining is localized to the membrane.
Left panel: with primary antibody at 1 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
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