Mouse Recombinant Monoclonal Thrombomodulin antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human THBD aa 50-200.
Images
![Protein Array - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (AB237912), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-rthbd-1591-bsa-and-azide-free-ab237912--protein-array-img137280.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (AB237912)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (AB237912), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-rthbd-1591-bsa-and-azide-free-ab237912--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108593.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (AB237912)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (AB237912), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-rthbd-1591-bsa-and-azide-free-ab237912--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134193.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (AB237912)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human THBD aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P07204
Reactivity data
Select an application| Protein Array | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Incubate with primary ab for 30 minutes at RT Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed:10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed:29323190, PubMed:33836597, PubMed:9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed:26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed:15841214).
Alternative names
CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin
Recommended products
Mouse Recombinant Monoclonal Thrombomodulin antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human THBD aa 50-200.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human THBD aa 50-200. The exact immunogen used to generate this antibody is proprietary information. Database link P07204
- Carrier free
- Yes
- Clone number
- rTHBD/1591
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Ab purified from Bioreactor Concentrate by Protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.
- Biological function summary
Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.
- Pathways
Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.
- Associated diseases and disorders
Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
![Protein Array - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (ab237912), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-rthbd-1591-bsa-and-azide-free-ab237912--protein-array-img137280.jpg "Protein Array - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (ab237912)")
Protein Array - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (ab237912)
This data was produced with Anti-Thrombomodulin antibody [rTHBD/1591] ab238061, the same antibody in a different formulation with BSA and Azide.
Anti-Thrombomodulin antibody [rTHBD/1591] ab238061 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (ab237912), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-rthbd-1591-bsa-and-azide-free-ab237912--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img108593.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (ab237912)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (ab237912)
Formalin-fixed, paraffin-embedded human placenta tissue stained for Thrombomodulin using ab237912 at 2 μg/mL in immunohistochemical analysis.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (ab237912), expandable thumbnail](https://content.abcam.com/products/images/thrombomodulin-antibody-rthbd-1591-bsa-and-azide-free-ab237912--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134193.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (ab237912)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [rTHBD/1591] - BSA and Azide free (ab237912)
Formalin-fixed, paraffin-embedded human cervical carcinoma tissue stained for Thrombomodulin using ab237912 at 2 μg/mL in immunohistochemical analysis.
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