AB227701

Anti-Thrombomodulin antibody [SP309] - C-terminal

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Thrombomodulin antibody. C-terminal. Suitable for Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin

6 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human cervical squamous cell carcinoma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human placenta tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human skin squamous cell carcinoma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human lung squamous cell carcinoma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Formalin-fixed paraffin-embedded human mesothelioma tissue stained for Thrombomodulin using ab227701 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Flow cytometric analysis of THP-1 (human monocytic leukemia cell line) cell line labeling Thrombomodulin with ab227701 at 1/300 dilution (green) compared with a rabbit IgG negative control (blue).

Carrier free

Anti-Thrombomodulin antibody [SP309] - BSA and Azide free
578 PE

PE Anti-Thrombomodulin antibody [SP309] (C-terminal)
660 APC

APC Anti-Thrombomodulin antibody [SP309] (C-terminal)
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Thrombomodulin antibody [SP309] - C-terminal
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Thrombomodulin antibody [SP309] - C-terminal
603 Alexa Fluor® 568

Alexa Fluor® 568 Anti-Thrombomodulin antibody [SP309] - C-terminal
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-Thrombomodulin antibody [SP309] - C-terminal
HRP

HRP Anti-Thrombomodulin antibody [SP309] (C-terminal)
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-Thrombomodulin antibody [SP309] - C-terminal
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-Thrombomodulin antibody [SP309] - C-terminal

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP309

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/300", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.099% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.

Biological function summary

Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.

Pathways

Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.

Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed : 10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed : 29323190, PubMed : 33836597, PubMed : 9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed : 26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed : 15841214).

See full target information THBD

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell reports. Medicine 5:101852 PubMed39657664

2024

Lurbinectedin sensitizes PD-L1 blockade therapy by activating STING-IFN signaling in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Subhamoy Chakraborty,Utsav Sen,Kedwin Ventura,Vrinda Jethalia,Charles Coleman,Subhasree Sridhar,Avisek Banerjee,Hilal Ozakinci,Yazhini Mahendravarman,Konrad Snioch,Elisa de Stanchina,Misty D Shields,Lewis E Tomalin,Deniz Demircioglu,Theresa A Boyle,Anna Tocheva,Dan Hasson,Triparna Sen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thrombomodulin antibody [SP309] - C-terminal

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

Flow Cytometry (Intracellular) - Anti-Thrombomodulin antibody [SP309] - C-terminal (AB227701)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise