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AB269746

Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free

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Mouse Monoclonal Thrombomodulin antibody. Carrier free. Suitable for WB, Protein Array, IHC-P and reacts with Human, Recombinant full length protein - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human THBD aa 50-200.

View Alternative Names

CD141, THRM, THBD, Thrombomodulin, TM, Fetomodulin

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human cervical carcinoma tissue labeling Thrombomodulin with ab218327 at 2 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human bladder carcinoma tissue labeling Thrombomodulin with ab218327 at 2 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218327)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling Thrombomodulin with ab218327 at 2 μg/ml.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218327)

Protein Array - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)
  • Protein Array

Unknown

Protein Array - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)

ab218327 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score : The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218327)

Western blot - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)
  • WB

Supplier Data

Western blot - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (AB269746)

All lanes:

Western blot - Anti-Thrombomodulin antibody [THBD/1591] - BSA and Azide free (ab269746) at 2 µg/mL

Lane 1:

Recombinant Protein

Lane 2:

THP1 cell lysate

Predicted band size: 60 kDa

Observed band size: 100 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

THBD/1591

Isotype

IgG2b

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

Protein Array, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human THBD aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P07204

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ProteinArray" : {"fullname" : "Protein Array", "shortname":"Protein Array"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ProteinArray-species-checked": "guaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 minutes at RT).</p>" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ProteinArray-species-checked": "testedAndGuaranteed", "ProteinArray-species-dilution-info": "", "ProteinArray-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

Product details

ab269746 is a carrier free version of ab218327.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab218327 was purified from Bioreactor Concentrate by Protein A/G.
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombomodulin also known as CD141 is a glycoprotein with a mass of approximately 74 kDa. It is an integral membrane protein expressed on the surface of endothelial cells. Thrombomodulin acts mechanically by binding thrombin an enzyme involved in blood coagulation to modulate its activity. This binding substantially alters thrombin's substrate specificity turning it into an anticoagulant enzyme that activates protein C instead of a procoagulant enzyme.
Biological function summary

Thrombomodulin regulates blood coagulation and maintains hemostatic balance. It forms a complex with thrombin on endothelial cells facilitating the conversion of protein C to activated protein C (APC). Activated protein C plays a pivotal role in controlling blood clot formation by proteolytically inactivating Factors Va and VIIIa. This function is important for preventing excessive clotting while ensuring proper wound healing processes.

Pathways

Thrombomodulin works chiefly within the anticoagulation pathway. It interacts directly with the thrombin-protein C pathway to achieve the activation of protein C. Additionally it interfaces with other pathways sharing mutual components such as endothelial cell proliferation and inflammation modulation. Related proteins include thrombin and protein C along with protein S which serves as a cofactor for APC's anticoagulant actions.

Thrombomodulin dysregulation can lead to thrombosis and vascular inflammation. A deficiency or altered expression of thrombomodulin has been linked to thromboembolic disorders and atherosclerosis. In these conditions improper activation of protein C in the presence of decreased thrombomodulin function can exacerbate clot formation. Monitoring the levels of proteins like thrombin and protein C within these diseases can provide insights into endothelial dysfunction and potential therapeutic approaches.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Endothelial cell receptor that plays a critical role in regulating several physiological processes including hemostasis, coagulation, fibrinolysis, inflammation, and angiogenesis (PubMed : 10761923). Acts as a cofactor for thrombin activation of protein C/PROC on the surface of vascular endothelial cells leading to initiation of the activated protein C anticoagulant pathway (PubMed : 29323190, PubMed : 33836597, PubMed : 9395524). Also accelerates the activation of the plasma carboxypeptidase B2/CPB2, which catalyzes removal of C-terminal basic amino acids from its substrates including kinins or anaphylatoxins leading to fibrinolysis inhibition (PubMed : 26663133). Plays critical protective roles in changing the cleavage specificity of protease-activated receptor 1/PAR1, inhibiting endothelial cell permeability and inflammation (By similarity). Suppresses inflammation distinctly from its anticoagulant cofactor activity by sequestering HMGB1 thereby preventing it from engaging cellular receptors such as RAGE and contributing to the inflammatory response (PubMed : 15841214).
See full target information THBD

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