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AB40664

Anti-Thrombopoietin antibody

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(1 Publication)

Rabbit Polyclonal Thrombopoietin antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human THPO aa 250-350 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

MGDF, THPO, Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human THPO aa 250-350 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P40225

Specificity

Cross reactivity: Thrombopoietin Human (299-332) 100%; Thrombopoietin Human recombinant ~90% Does not cross react with Erythropoietin Human recombinant.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.</p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombopoietin also known as TPO is an important glycoprotein hormone with a mass of about 70 kDa. It is primarily produced in the liver but can also be found in the kidneys and bone marrow stromal cells. Thrombopoietin binds to and activates its receptor c-Mpl which is present on hematopoietic progenitor cells. This protein has a significant role in regulating the production of blood platelets by stimulating the proliferation and differentiation of megakaryocytes the precursor cells for platelets.
Biological function summary

Thrombopoietin functions in hematopoiesis by supporting the maturation of megakaryocytes. It operates independently but can interact with other factors to optimize platelet production. Although it does not form a part of a large protein complex it is essential for the proper function of hematopoietic progenitors across several stages of differentiation. Its activity ensures the maintenance of platelet levels in the bloodstream therefore preventing conditions like thrombocytopenia.

Pathways

Thrombopoietin plays a critical role in the megakaryocyte/platelet lineage pathway. It directly influences the JAK-STAT (Janus kinase-signal transducer and activator of transcription) signaling pathway which is vital for driving the cell cycle and promoting cell survival. Thrombopoietin shares a functional connection with EPO (erythropoietin) which is involved in red blood cell production although they act on different lineage-specific progenitors.

Thrombopoietin is intricately linked to conditions like thrombocytopenia where there are abnormally low platelet counts and thrombocythemia characterized by elevated platelet levels. Dysregulation of Thrombopoietin production or its receptor activity can lead to these disorders. It has a relationship with other hematopoietic growth factors like GM-CSF (granulocyte-macrophage colony-stimulating factor) which can also influence the disorders of blood cell lines. Understanding its signaling pathways provides insights into therapeutic targets for diseases involving platelet imbalances.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
See full target information THPO
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Infection and immunity 79:928-36 PubMed21149592

2010

Thrombopoietin contributes to neuronal damage in experimental bacterial meningitis.

Applications

IHC-P

Species

Human

Olaf Hoffmann,Olga Rung,Ae-Rie Im,Dorette Freyer,Juan Zhang,Josephin Held,Werner Stenzel,Christof Dame
View all publications
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