Rabbit Recombinant Monoclonal Thrombopoietin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (AB196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29563.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (AB196026)")
![Flow Cytometry (Intracellular) - Anti-Thrombopoietin antibody [EPR14948] (AB196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--flow-cytometry-intracellular-img50277.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Thrombopoietin antibody [EPR14948] (AB196026)")
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombopoietin antibody [EPR14948] (AB196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--immunocytochemistry-immunofluorescence-img126069.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Thrombopoietin antibody [EPR14948] (AB196026)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (AB196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24217.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (AB196026)")
![Western blot - Anti-Thrombopoietin antibody [EPR14948] (AB196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--western-blot-img84954.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thrombopoietin antibody [EPR14948] (AB196026)")
Publications
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- Cancer gene therapy 29:1742-17502022Combination regimen of granulocyte colony-stimulating factor and recombinant human thrombopoietin improves the curative effect on elderly patients with leukemia through inducing pyroptosis and ferroptosis of leukemia cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaobin Wang,Xiaoyu Liu,Huihan WangPubMed 35768562
- Journal of thrombosis and haemostasis : JTH 20:1900-19092022Generation of a thrombopoietin-deficient thrombocytopenia model in zebrafish.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLian Yang,Liangliang Wu,Panpan Meng,Xuebing Zhang,Dejian Zhao,Qing Lin,Yiyue ZhangPubMed 35622056
- Wellcome open research 6:832021Hematological consequences of malaria in mice previously treated for visceral leishmaniasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGulab Fatima Rani,Helen Ashwin,Najmeeyah Brown,Ian S Hitchcock,Paul M KayePubMed 34286101
- Revista da Associacao Medica Brasileira (1992) 66:590-5952020Thrombopoietin is associated with a prognosis of gastric adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChang-Lin Zhou et. al.PubMed 32638965
- Journal of cellular and molecular medicine 24:6644-66572020Endogenous thrombopoietin promotes non-small-cell lung carcinoma cell proliferation and migration by regulating EGFR signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZifang Zou,Xiaoxi Fan,Yang Liu,Yanbin Sun,Xin Zhang,Guanghao Sun,Xuehao Li,Shun XuPubMed 32337844
- Journal of cancer research and clinical oncology 145:1147-11642019CD110 promotes pancreatic cancer progression and its expression is correlated with poor prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZilong Yan,Kenoki Ohuchida,Biao Zheng,Takashi Okumura,Shin Takesue,Hiromichi Nakayama,Chika Iwamoto,Koji Shindo,Taiki Moriyama,Kohei Nakata,Yoshihiro Miyasaka,Takao Ohtsuka,Kazuhiro Mizumoto,Yoshinao Oda,Makoto Hashizume,Masafumi NakamuraPubMed 30770989
- EMBO molecular medicine 10:63-752017Thrombopoietin mutation in congenital amegakaryocytic thrombocytopenia treatable with romiplostim.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessandro Pecci,Iman Ragab,Valeria Bozzi,Daniela De Rocco,Serena Barozzi,Tania Giangregorio,Heba Ali,Federica Melazzini,Mohamed Sallam,Caterina Alfano,Annalisa Pastore,Carlo L Balduini,Anna SavoiaPubMed 29191945
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Recombinant full length protein - Human | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Recombinant full length protein - Human | Dilution info 1/1000 - 1/5000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/40 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Alternative names
Thrombopoietin, C-mpl ligand, Megakaryocyte colony-stimulating factor, Megakaryocyte growth and development factor, Myeloproliferative leukemia virus oncogene ligand, ML, MGDF, MGDF, THPO
Recommended products
Rabbit Recombinant Monoclonal Thrombopoietin antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Recombinant full length protein - Human samples. Cited in 7 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR14948
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (ab196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img29563.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (ab196026)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (ab196026)
Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cytoplasm staining on human bone marrow tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-Thrombopoietin antibody [EPR14948] (ab196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--flow-cytometry-intracellular-img50277.jpg "Flow Cytometry (Intracellular) - Anti-Thrombopoietin antibody [EPR14948] (ab196026)")
Flow Cytometry (Intracellular) - Anti-Thrombopoietin antibody [EPR14948] (ab196026)
Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Thrombopoietin with purified ab196026 at 1/40 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
![Immunocytochemistry/ Immunofluorescence - Anti-Thrombopoietin antibody [EPR14948] (ab196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--immunocytochemistry-immunofluorescence-img126069.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Thrombopoietin antibody [EPR14948] (ab196026)")
Immunocytochemistry/ Immunofluorescence - Anti-Thrombopoietin antibody [EPR14948] (ab196026)
Immunofluorescent analysis of Jurkat cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Thrombopoietin with ab196026 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).
Note: Cytoplasm staining on Jurkat cell line was observed.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (ab196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img24217.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (ab196026)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] (ab196026)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset: negative control).
Note: Cytoplasm staining on human liver tissue was observed.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--western-blot-img84954.jpg "Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026)")
Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026)
All lanes: Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/5000 dilution
All lanes: Thrombopoietin recombinant protein at 0.01 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 37 kDa
![Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--western-blot-img157820.jpg "Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026)")
Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026)
All lanes: Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026) at 1/1000 dilution
All lanes: Human fetal liver at 10 µg
SecondaryAll lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 37 kDa
![Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026), expandable thumbnail](https://content.abcam.com/products/images/thrombopoietin-antibody-epr14948-ab196026--western-blot-img157820.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thrombopoietin antibody [EPR14948] (ab196026)")
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