Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (AB251218)

This data was developed using ab196026, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset : negative control).

Note : Cytoplasm staining on human liver tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (AB251218)

This data was developed using ab196026, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human bone marrow tissue labeling Thrombopoietin with ab196026 at 1/4000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution and counter-stained with Hematoxylin. (inset : negative control).

Note : Cytoplasm staining on human bone marrow tissue was observed.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (AB251218)

This data was developed using ab196026, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of Jurkat (human acute T cell leukemia) labelling Thrombopoietin with purified ab196026 at 1/40 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Alexa Fluor^® 488 goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (AB251218)

This data was developed using ab196026, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of Jurkat cells (4% Paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized) labeling Thrombopoietin with ab196026 at 1/50 dilution followed by Goat anti rabbit IgG (AlexaFluor® 488) (ab150077) secondary at 1/400 dilution and counter-stained with DAPI (blue).

Note : Cytoplasm staining on Jurkat cell line was observed.

• WB

Supplier Data

Western blot - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (AB251218)

This data was developed using ab196026, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Thrombopoietin antibody [EPR14948] (<a href='/en-us/products/primary-antibodies/thrombopoietin-antibody-epr14948-ab196026'>ab196026</a>) at 1/1000 dilution

All lanes:

Human fetal liver at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 37 kDa

false

• WB

Supplier Data

Western blot - Anti-Thrombopoietin antibody [EPR14948] - BSA and Azide free (AB251218)

This data was developed using ab196026, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Thrombopoietin antibody [EPR14948] (<a href='/en-us/products/primary-antibodies/thrombopoietin-antibody-epr14948-ab196026'>ab196026</a>) at 1/5000 dilution

All lanes:

Thrombopoietin recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 37 kDa

false