Name: Anti-Thrombospondin 1 antibody [EPR22927-54]
SKU: ab267388
Rating: 2 (1 reviews)

Rabbit Recombinant Monoclonal Thrombospondin 1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 9 publications.

Images

Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] (AB267388), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Expected	Tested	Tested
Rat	Expected	Tested	Tested	Tested	Expected

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Rat	Dilution info 1/100	Notes -
Species Human	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/50 - 1/500	Notes -
Species Human	Dilution info 1/50 - 1/500	Notes -
Species Rat	Dilution info 1/50 - 1/500	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

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Target data

See full target information THBS1

Function

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions (PubMed:15014436, PubMed:18285447, PubMed:2430973, PubMed:6489349). Multifunctional, involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and cardiovascular and metabolic homeostasis (PubMed:10613822, PubMed:11134179, PubMed:1371676, PubMed:14568985, PubMed:24511121, PubMed:29042481, PubMed:32679764). Negatively modulates dendritic cell activation and cytokine release, as part of an autocrine feedback loop, contributing to the resolution of inflammation and immune homeostasis (PubMed:14568985). Ligand for receptor CD47 (PubMed:19004835, PubMed:8550562). Modulates nitrous oxide (NO) signaling via CD47, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays a role in endothelial cell senescence, acting via CD47, by increasing the abundance and activation of NADPH oxidase NOX1, and so generating excess ROS (PubMed:29042481). Inhibits stem cell self-renewal, acting via CD47 signaling, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). Negatively modulates wound healing, acting via CD47 (By similarity). Ligand for receptor CD36 (PubMed:10613822, PubMed:11134179, PubMed:1371676). Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting via CD36 (By similarity). Plays a role in suppressing angiogenesis, acting, depending on context, via CD36 or CD47 (PubMed:10613822, PubMed:11134179, PubMed:1371676, PubMed:32679764). Promotes cellular senescence in a TP53-CDKN1A-RB1 signaling-dependent manner (PubMed:29042481). Ligand for immunoglobulin-like cell surface receptor SIRPA (PubMed:24511121). Involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production, acting via interaction with SIRPA (PubMed:24511121). Plays a role in metabolic dysfunction in diet-induced obesity, perhaps acting by exacerbating adipose inflammatory activity; its effects may be mediated, at least in part, through enhanced adipocyte proliferation (By similarity). Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). May be involved in age-related conditions, including metabolic dysregulation, during normal aging (PubMed:29042481, PubMed:32679764).

Alternative names

TSP, TSP1, THBS1, Thrombospondin-1, Glycoprotein G

Recommended products

Rabbit Recombinant Monoclonal Thrombospondin 1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 9 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR22927-54
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Thrombospondin 1 also known as TSP1 is a multi-functional glycoprotein involved in cellular processes like adhesion migration and tumorigenesis. It has a molecular weight of approximately 450 kDa. TSP1 is produced by multiple cell types including endothelial cells and fibroblasts and it is widely expressed in the extracellular matrix of both normal and tumor tissues. As a member of the thrombospondin family it interacts with cell receptors like integrins and CD36 to mediate its diverse functions.

Biological function summary

TSP1 is significant in modulating angiogenesis immune response and apoptosis. It acts as an anti-angiogenic factor by inhibiting endothelial cell proliferation and migration. TSP1 is a part of a larger complex where it interacts with other matrix proteins and growth factors to fine-tune cellular behavior within tissues. Through its binding domains it inhibits matrix metalloproteinases helping regulate matrix composition and tissue remodeling.

Pathways

TSP1 influences both the TGF-beta and NF-kB signaling pathways. In the TGF-beta pathway TSP1 activates latent TGF-beta contributing to tissue repair and fibrosis processes. It works synergistically with proteins like SMADs in these pathways. In the NF-kB pathway TSP1 modulates immune responses and inflammatory processes. Its interaction with CD36 and integrins in these pathways underlines its versatile role in cellular signaling.

Associated diseases and disorders

TSP1 has been investigated in cancer progression and cardiovascular diseases. In cancer TSP1's anti-angiogenic properties suppress tumor vascularization which can slow tumor growth. Aberrant TSP1 expression levels have also been linked to atherosclerosis development as it interacts with endothelial cells and inflammatory molecules like TNF-alpha. In both these contexts TSP1 engages with proteins such as integrins and CD36 to exert its influence on disease progression.

Product promise

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10 product images

Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.Thrombospondin 1 was immunoprecipitated from 0.35 mg Mouse platelets whole cell lysate 10ug with ab267388 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267388 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse platelets whole cell lysate 10ug
Lane 2: ab267388 IP in Mouse platelets whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab267388 in Mouse platelets whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
All lanes: Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
Predicted band size: 129 kDa
Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.Thrombospondin 1 was immunoprecipitated from 0.35 mg HUVEC (Human umbilical vein endothelial cell) whole cell lysate 10ug with ab267388 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267388 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HUVEC (Human umbilical vein endothelial cell) whole cell lysate 10ug
Lane 2: ab267388 IP in HUVEC whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab267388 in HUVEC whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
All lanes: Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
Predicted band size: 129 kDa
Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
The full-length TSP 1 (180kDa) and a 160-kDa band, likely to be an TSP 1 isoform or fragment, are observed.
The molecular weight observed is consistent with what has been described in the literature (PMID:1426766, 27588705).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388) at 1/1000 dilution
Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg
Lane 2: Mouse platelet lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 129 kDa
Exposure time: 3min
Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
TSP 1 is a serum-responsive gene. Its express is elevated in quiescent 3T3 with serum addition.
The full-length TSP 1 (180kDa) and a 160-kDa band, likely to be an TSP 1 isoform or fragment, are observed.
The molecular weight observed is consistent with what has been described in the literature (PMID:1426766, 27588705).
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388) at 1/1000 dilution
Lane 1: 3T3-L1 (mouse embryonic fibroblast) starved with 0.4% serum for 30 hours whole cell lysate at 10 µg
Lane 2: 3T3-L1 starved with 0.4% serum for 24 hours, then cultivated with 15% serum for 6 hours. whole cell lysate at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 129 kDa
Exposure time: 3min
Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling Thrombospondin 1 with ab267388 at 1/50 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor� 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
Intracellular Flow Cytometry analysis of PC-12 (Rat adrenal gland pheochromocytoma cell line) cells labeling Thrombospondin 1 with ab267388 at 1/500 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat Anti-rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1/5000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST
The full-length TSP 1 (180 kDa) and a ~140 kDa band, likely to be a TSP 1 isoform or fragment, are observed.
The molecular weight observed is consistent with what has been described in the literature (PMID:1426766, 27588705).
Lane 1: Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free ab267397) at 1/1000 dilution
Lane 1: Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388) at 1/1000 dilution
All lanes: Rat platelet whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 129 kDa
Observed band size: 140 kDa, 180 kDa
Exposure time: 5.5s
Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 3T3-L1 (mouse embryonic fibroblast) starved with 0.4% serum for 24h, then cultured with 15% serum for 6h (Red) / Untreated control (Green) cells labelling Thrombospondin 1 with ab267388 at 1/50 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor� 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Thrombospondin 1 with ab267388 at 1/100 (5.3 μg/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor_® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor_® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 AlexaFluor_® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] (ab267388)
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling Thrombospondin 1 with ab267388 at 1/5000 (0.101 μg/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at a Ready to use dilution. Positive staining on extracellular matrix of human cervical carcinoma. The section was incubated with ab267388 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) at Ready to use dilution.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.