AB267397

Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Thrombospondin 1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

TSP, TSP1, THBS1, Thrombospondin-1, Glycoprotein G

14 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labelling Thrombospondin 1 with ab267388 at 1/5000 (0.101 μg/ml) followed by a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at a Ready to use dilution. Positive staining on extracellular matrix of human cervical carcinoma. The section was incubated with ab267388 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388. Immunohistochemical analysis of paraffin-embedded human bone marrow tissue labeling Thrombospondin 1 with ab267388 at 1/5000 dilution (0.1 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the megakaryocytes in the human bone marrow (PMID : 28239144). The section was incubated with ab267388 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HUVEC (human umbilical vein endothelial cell) cells labeling Thrombospondin 1 with ab267388 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388. Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Thrombospondin 1 with ab267388 at 1/5000 dilution (0.1 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the platelets in the human spleen (PMID : 28239144). The section was incubated with ab267388 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

Unknown

Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

Thrombospondin 1 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate 10μg with ab267388 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267388 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HUVEC whole cell lysate 10μg.

Lane 2 : ab267388 IP in HUVEC whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab267388 in HUVEC whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds.

All lanes:

Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] (<a href='/en-us/products/primary-antibodies/thrombospondin-1-antibody-epr22927-54-ab267388'>ab267388</a>)

Predicted band size: 129 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunofluorescent analysis of 100% methanol-fixed, permeabilized HUVEC (human umbilical vein endothelial cell) cells labeling Thrombospondin 1 with ab267388 at 1/100 dilution (5 µg/ml), followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in HUVEC cell line is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution. 100% methanol fixation is recommended. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized 3T3-L1 (mouse embryonic fibroblast) starved with 0.4% serum for 24h, then cultured with 15% serum for 6h (Red) / Untreated control (Green) cells labeling Thrombospondin 1 with ab267388 at 1/50 (Red) compared with a Rabbit monoclonal IgG (ab172730) / Black isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Intracellular Flow Cytometry analysis of PC-12 (Rat adrenal gland pheochromocytoma cell line) cells labeling Thrombospondin 1 with ab267388 at 1/500 dilution (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat Anti-rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1/5000 dilution. Isotype control - Rabbit monoclonal IgG (Black) (ab172730). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Thrombospondin 1 with ab267388 at 1/5000 dilution (0.1 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Positive staining on the megakaryocytes and platelets in the mouse spleen is observed. The section was incubated with ab267388 for 10 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP Polymer). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunofluorescent analysis of 100% Methanol-fixed, 0.1% TritonX-100 permeabilized PC-12 (rat adrenal gland pheochromocytoma cell) cells labelling Thrombospondin 1 with ab267388 at 1/100 (5.3 μg/ml) dilution, followed by ab150077 AlexaFluor_® 488 Goat anti-Rabbit secondary antibody at 1/1000 (2 μg/ml) dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor_® 594) was used to counterstain tubulin at 1/200 (2.5 μg/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor_® 488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution. This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

Unknown

Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

Thrombospondin 1 was immunoprecipitated from 0.35 mg mouse platelets whole cell lysate 10μg with ab267388 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab267388 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse platelets whole cell lysate 10μg.

Lane 2 : ab267388 IP in mouse platelets whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab267388 in mouse platelets whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds.

All lanes:

Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] (<a href='/en-us/products/primary-antibodies/thrombospondin-1-antibody-epr22927-54-ab267388'>ab267388</a>)

Predicted band size: 129 kDa

false

Supplier Data

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

The full-length TSP 1 (180kDa) and a 160-kDa band, likely to be an TSP 1 isoform or fragment, are observed.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1426766, 27588705).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] (<a href='/en-us/products/primary-antibodies/thrombospondin-1-antibody-epr22927-54-ab267388'>ab267388</a>) at 1/1000 dilution

Lane 1:

HUVEC (human umbilical vein endothelial cell) whole cell lysate at 20 µg

Lane 2:

Mouse platelet lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 129 kDa

false

Exposure time: 3min

Unknown

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

TSP 1 is a serum-responsive gene. Its express is elevated in quiescent 3T3 with serum addition.

The full-length TSP 1 (180kDa) and a 160-kDa band, likely to be an TSP 1 isoform or fragment, are observed.

The molecular weight observed is consistent with what has been described in the literature (PMID : 1426766, 27588705).

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] (<a href='/en-us/products/primary-antibodies/thrombospondin-1-antibody-epr22927-54-ab267388'>ab267388</a>) at 1/1000 dilution

Lane 1:

3T3-L1 (mouse embryonic fibroblast) starved with 0.4% serum for 30 hours whole cell lysate at 10 µg

Lane 2:

3T3-L1 starved with 0.4% serum for 24 hours, then cultivated with 15% serum for 6 hours. whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 129 kDa

false

Exposure time: 3min

Lab

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM/TBST The full-length TSP 1 (180 kDa) and a ~140 kDa band, likely to be a TSP 1 isoform or fragment, are observed. The molecular weight observed is consistent with what has been described in the literature (PMID : 1426766, 27588705). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide ab267388.

Lane 1:

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (ab267397) at 1/1000 dilution

Lane 1:

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] (<a href='/en-us/products/primary-antibodies/thrombospondin-1-antibody-epr22927-54-ab267388'>ab267388</a>) at 1/1000 dilution

All lanes:

Rat platelet whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 129 kDa

Observed band size: 140 kDa,180 kDa

false

Exposure time: 5.5s

Unconjugated

Anti-Thrombospondin 1 antibody [EPR22927-54]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22927-54

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse, Human

Applications

Flow Cyt (Intra), ICC/IF, IP, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab267397 is the carrier-free version of ab267388.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thrombospondin 1 also known as TSP1 is a multi-functional glycoprotein involved in cellular processes like adhesion migration and tumorigenesis. It has a molecular weight of approximately 450 kDa. TSP1 is produced by multiple cell types including endothelial cells and fibroblasts and it is widely expressed in the extracellular matrix of both normal and tumor tissues. As a member of the thrombospondin family it interacts with cell receptors like integrins and CD36 to mediate its diverse functions.

Biological function summary

TSP1 is significant in modulating angiogenesis immune response and apoptosis. It acts as an anti-angiogenic factor by inhibiting endothelial cell proliferation and migration. TSP1 is a part of a larger complex where it interacts with other matrix proteins and growth factors to fine-tune cellular behavior within tissues. Through its binding domains it inhibits matrix metalloproteinases helping regulate matrix composition and tissue remodeling.

Pathways

TSP1 influences both the TGF-beta and NF-kB signaling pathways. In the TGF-beta pathway TSP1 activates latent TGF-beta contributing to tissue repair and fibrosis processes. It works synergistically with proteins like SMADs in these pathways. In the NF-kB pathway TSP1 modulates immune responses and inflammatory processes. Its interaction with CD36 and integrins in these pathways underlines its versatile role in cellular signaling.

TSP1 has been investigated in cancer progression and cardiovascular diseases. In cancer TSP1's anti-angiogenic properties suppress tumor vascularization which can slow tumor growth. Aberrant TSP1 expression levels have also been linked to atherosclerosis development as it interacts with endothelial cells and inflammatory molecules like TNF-alpha. In both these contexts TSP1 engages with proteins such as integrins and CD36 to exert its influence on disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Adhesive glycoprotein that mediates cell-to-cell and cell-to-matrix interactions (PubMed : 15014436, PubMed : 18285447, PubMed : 2430973, PubMed : 6489349). Multifunctional, involved in inflammation, angiogenesis, wound healing, reactive oxygen species (ROS) signaling, nitrous oxide (NO) signaling, apoptosis, senescence, aging, cellular self-renewal, stemness, and cardiovascular and metabolic homeostasis (PubMed : 10613822, PubMed : 11134179, PubMed : 1371676, PubMed : 14568985, PubMed : 24511121, PubMed : 29042481, PubMed : 32679764). Negatively modulates dendritic cell activation and cytokine release, as part of an autocrine feedback loop, contributing to the resolution of inflammation and immune homeostasis (PubMed : 14568985). Ligand for receptor CD47 (PubMed : 19004835, PubMed : 8550562). Modulates nitrous oxide (NO) signaling via CD47, hence playing a role as a pressor agent, supporting blood pressure (By similarity). Plays a role in endothelial cell senescence, acting via CD47, by increasing the abundance and activation of NADPH oxidase NOX1, and so generating excess ROS (PubMed : 29042481). Inhibits stem cell self-renewal, acting via CD47 signaling, probably by regulation of the stem cell transcription factors POU5F1/OCT4, SOX2, MYC/c-Myc and KLF4 (By similarity). Negatively modulates wound healing, acting via CD47 (By similarity). Ligand for receptor CD36 (PubMed : 10613822, PubMed : 11134179, PubMed : 1371676). Involved in inducing apoptosis in podocytes in response to elevated free fatty acids, acting via CD36 (By similarity). Plays a role in suppressing angiogenesis, acting, depending on context, via CD36 or CD47 (PubMed : 10613822, PubMed : 11134179, PubMed : 1371676, PubMed : 32679764). Promotes cellular senescence in a TP53-CDKN1A-RB1 signaling-dependent manner (PubMed : 29042481). Ligand for immunoglobulin-like cell surface receptor SIRPA (PubMed : 24511121). Involved in ROS signaling in non-phagocytic cells, stimulating NADPH oxidase-derived ROS production, acting via interaction with SIRPA (PubMed : 24511121). Plays a role in metabolic dysfunction in diet-induced obesity, perhaps acting by exacerbating adipose inflammatory activity; its effects may be mediated, at least in part, through enhanced adipocyte proliferation (By similarity). Plays a role in ER stress response, via its interaction with the activating transcription factor 6 alpha (ATF6) which produces adaptive ER stress response factors (By similarity). May be involved in age-related conditions, including metabolic dysregulation, during normal aging (PubMed : 29042481, PubMed : 32679764).

See full target information THBS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

NPJ precision oncology 8:278 PubMed39627379

2024

Assessing CD36 and CD47 expression levels in solid tumor indications to stratify patients for VT1021 treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Suming Wang,Victor Zota,Melanie Y Vincent,Donna Clossey,Jian Jenny Chen,Michael Cieslewicz,Randolph S Watnick,James Mahoney,Jing Watnick

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Flow Cytometry (Intracellular) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunocytochemistry/ Immunofluorescence - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Immunoprecipitation - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Western blot - Anti-Thrombospondin 1 antibody [EPR22927-54] - BSA and Azide free (AB267397)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise