AB187176

Anti-Thromboxane synthase antibody

Name: Anti-Thromboxane synthase antibody (ab187176) | Abcam
Brand: Abcam Limited
SKU: AB187176

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(2 Publications)

Rabbit Polyclonal Thromboxane synthase antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TBXAS1 aa 350 to C-terminus.

View Alternative Names

CYP5, CYP5A1, TXAS, TBXAS1, Thromboxane-A synthase, TXA synthase, TXS, Cytochrome P450 5A1, Hydroperoxy icosatetraenoate dehydratase

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thromboxane synthase antibody (AB187176)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thromboxane synthase antibody (AB187176)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling Thromboxane synthase with ab187176 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thromboxane synthase antibody (AB187176)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling Thromboxane synthase with ab187176 at 1/200. Magnification : 400x.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thromboxane synthase antibody (AB187176)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling Thromboxane synthase with ab187176 at 1/200. Magnification : 400x.

Supplier Data

Western blot - Anti-Thromboxane synthase antibody (AB187176)

All lanes:

Western blot - Anti-Thromboxane synthase antibody (ab187176) at 1/500 dilution

Lane 1:

A549 cell extract

Lane 2:

HT-29 cell extract

Lane 3:

U937 cell extract

Lane 4:

H460 cell extract

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human TBXAS1 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P24557

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.3 Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thromboxane synthase also known as TBXAS1 is an enzyme that plays an important role in the biosynthesis of thromboxane A2 from prostaglandin H2. It is a 60 kDa protein primarily expressed in platelets and megakaryocytes as well as in cells within the lung kidney and spleen. By catalyzing the conversion of prostaglandin H2 to thromboxane A2 it influences the regulation of vasoconstriction and platelet aggregation.

Biological function summary

Thromboxane A2 generated by thromboxane synthase acts as a potent vasoconstrictor and promotes platelet aggregation important for hemostasis. This enzyme does not function as part of a larger complex but independently facilitates thromboxane A2 production. Its action significantly contributes to cardiovascular function where it modulates vascular tone and platelet activity.

Pathways

Thromboxane synthase is integral to the arachidonic acid metabolic pathway and the eicosanoid signaling pathway. The enzyme interacts closely with cyclooxygenase enzymes COX-1 and COX-2 in these pathways as they provide the prostaglandin H2 substrate for thromboxane A2 synthesis. By influencing these pathways thromboxane synthase indirectly affects inflammation and cardiovascular regulation.

The activity of thromboxane synthase is associated with cardiovascular diseases and disorders such as myocardial infarction and stroke. Overproduction of thromboxane A2 facilitated by this enzyme can lead to excessive platelet aggregation and vasoconstriction contributing to these conditions. Thromboxane synthase is also related to COX-2 which is implicated in inflammatory responses and is a target for anti-inflammatory drugs like aspirin and other COX inhibitors.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation (PubMed : 11097184, PubMed : 11297515, PubMed : 22735388, PubMed : 24009185, PubMed : 8436233, PubMed : 9873013). Also cleaves PGH2 to 12-hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2 (PubMed : 11297515, PubMed : 22735388, PubMed : 9873013). Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and 15-HETE (PubMed : 17459323).

See full target information TBXAS1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 15:2037-2050 PubMed31592228

2019

Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Li,Zhenli Liu,Linlin Liu,Wen Li,Zhiwen Cao,Zhiqian Song,Qianqian Yang,Aiping Lu,Cheng Lu,Yuanyan Liu

Cell reports 27:3062-3080.e11 PubMed31167148

2019

Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Cianciaruso,Tim Beltraminelli,Florent Duval,Sina Nassiri,Romain Hamelin,André Mozes,Hector Gallart-Ayala,Gerardo Ceada Torres,Bruno Torchia,Carola H Ries,Julijana Ivanisevic,Michele De Palma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thromboxane synthase antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thromboxane synthase antibody (AB187176)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thromboxane synthase antibody (AB187176)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thromboxane synthase antibody (AB187176)

Western blot - Anti-Thromboxane synthase antibody (AB187176)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise