Rabbit Polyclonal Thromboxane synthase antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human TBXAS1 aa 350-400.
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- PloS one 11:e01516172016Lack of Thromboxane Synthase Prevents Hypertension and Fetal Growth Restriction after High Salt Treatment during Pregnancy.
- Atherosclerosis 246:98-1052016Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeegan A Walker,Birgitte Hoier,Philip J Walker,Karl Schulze,Jens Bangsbo,Ylva Hellsten,Christopher D AskewPubMed 26771386
- The Journal of biological chemistry 287:6454-682012Induction of COX-2 enzyme and down-regulation of COX-1 expression by lipopolysaccharide (LPS) control prostaglandin E2 production in astrocytes.Applications:WBReactive species:MouseMiriam Font-Nieves,M Glòria Sans-Fons,Roser Gorina,Ester Bonfill-Teixidor,Angélica Salas-Pérdomo,Leonardo Márquez-Kisinousky,Tomàs Santalucia,Anna M PlanasPubMed 22219191
- American journal of physiology. Heart and circulatory physiology 302:H479-882011Effects of cyclooxygenase inhibition on cardiovascular function in a hypercholesterolemic swine model of chronic ischemia.Applications:WBReactive species:PigLouis M Chu,Michael P Robich,Cesario Bianchi,Jun Feng,Yuhong Liu,Shu-Hua Xu,Thomas Burgess,Frank W SellkePubMed 22037194
- Hypertension (Dallas, Tex. : 1979) 58:943-92011Exercise training alters the balance between vasoactive compounds in skeletal muscle of individuals with essential hypertension.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAne H Hansen et. al.PubMed 21896936
- The Annals of thoracic surgery 92:829-362011Thromboxane-induced contractile response of human coronary arterioles is diminished after cardioplegic arrest.Applications:WBReactive species:HumanJun Feng,Yuhong Liu,Louis M Chu,Richard T Clements,Kamal R Khabbaz,Michael P Robich,Cesario Bianchi,Frank W SellkePubMed 21871266
- Free radical biology & medicine 51:1686-962011Inhibition of aldose reductase prevents endotoxin-induced inflammation by regulating the arachidonic acid pathway in murine macrophages.
- Inflammation research : official journal of the European Histamine Research Society ... [et al.] 60:87-922010Thromboxane-prostanoid receptor expression and antagonism in dextran-sodium sulfate-induced colitis.Applications:IHC-FrReactive species:MousePatsy R Carter,Robert M McElhatten,Songlin Zhang,William S Wright,Norman R HarrisPubMed 20694740
- Experimental eye research 89:532-72009Expression of thromboxane synthase and the thromboxane-prostanoid receptor in the mouse and rat retina.Applications:IHC-PReactive species:Rat, MouseWilliam S Wright,Robert M McElhatten,Norman R HarrisPubMed 19523949
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TBXAS1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link P24557
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Cynomolgus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cynomolgus monkey | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation (PubMed:11097184, PubMed:11297515, PubMed:22735388, PubMed:24009185, PubMed:8436233, PubMed:9873013). Cleaves also PGH2 to 12-hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2 (PubMed:11297515, PubMed:22735388, PubMed:9873013). Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and 15-HETE (PubMed:17459323).
Alternative names
CYP5, CYP5A1, TXAS, TBXAS1, Thromboxane-A synthase, TXA synthase, TXS, Cytochrome P450 5A1, Hydroperoxy icosatetraenoate dehydratase
Recommended products
Rabbit Polyclonal Thromboxane synthase antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human TBXAS1 aa 350-400.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TBXAS1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information. Database link P24557
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab39362 is an affinity purified IgG
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thromboxane synthase also known as TBXAS1 is an enzyme that plays an important role in the biosynthesis of thromboxane A2 from prostaglandin H2. It is a 60 kDa protein primarily expressed in platelets and megakaryocytes as well as in cells within the lung kidney and spleen. By catalyzing the conversion of prostaglandin H2 to thromboxane A2 it influences the regulation of vasoconstriction and platelet aggregation.
- Biological function summary
Thromboxane A2 generated by thromboxane synthase acts as a potent vasoconstrictor and promotes platelet aggregation important for hemostasis. This enzyme does not function as part of a larger complex but independently facilitates thromboxane A2 production. Its action significantly contributes to cardiovascular function where it modulates vascular tone and platelet activity.
- Pathways
Thromboxane synthase is integral to the arachidonic acid metabolic pathway and the eicosanoid signaling pathway. The enzyme interacts closely with cyclooxygenase enzymes COX-1 and COX-2 in these pathways as they provide the prostaglandin H2 substrate for thromboxane A2 synthesis. By influencing these pathways thromboxane synthase indirectly affects inflammation and cardiovascular regulation.
- Associated diseases and disorders
The activity of thromboxane synthase is associated with cardiovascular diseases and disorders such as myocardial infarction and stroke. Overproduction of thromboxane A2 facilitated by this enzyme can lead to excessive platelet aggregation and vasoconstriction contributing to these conditions. Thromboxane synthase is also related to COX-2 which is implicated in inflammatory responses and is a target for anti-inflammatory drugs like aspirin and other COX inhibitors.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-Thromboxane synthase antibody (ab39362)
All lanes: Western blot - Anti-Thromboxane synthase antibody (ab39362) at 1 µg/mL
Lane 1: Human spleen tissue lysate - total protein (ab29699) at 10 µg
Lane 2: WI38 (Human lung fibroblast cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 21 kDa, 36 kDa, 54 kDa
Exposure time: 8min
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