AB39362

Anti-Thromboxane synthase antibody

Name: Anti-Thromboxane synthase antibody (ab39362) | Abcam
Brand: Abcam Limited
SKU: AB39362

Be the first to review this product! Submit a review

(9 Publications)

Rabbit Polyclonal Thromboxane synthase antibody. Suitable for WB and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human TBXAS1 aa 350-400.

View Alternative Names

CYP5, CYP5A1, TXAS, TBXAS1, Thromboxane-A synthase, TXA synthase, TXS, Cytochrome P450 5A1, Hydroperoxy icosatetraenoate dehydratase

1 Images

Unknown

Western blot - Anti-Thromboxane synthase antibody (AB39362)

All lanes:

Western blot - Anti-Thromboxane synthase antibody (ab39362) at 1 µg/mL

Lane 1:

Human spleen tissue lysate - total protein (<a href='/en-us/products/unavailable/human-spleen-tissue-lysate-total-protein-ab29699'>ab29699</a>) at 10 µg

Lane 2:

WI38 (Human lung fibroblast cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 61 kDa

Observed band size: 21 kDa,36 kDa,54 kDa

true

Exposure time: 8min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human TBXAS1 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P24557

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab39362 is an affinity purified IgG

Storage buffer

Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thromboxane synthase also known as TBXAS1 is an enzyme that plays an important role in the biosynthesis of thromboxane A2 from prostaglandin H2. It is a 60 kDa protein primarily expressed in platelets and megakaryocytes as well as in cells within the lung kidney and spleen. By catalyzing the conversion of prostaglandin H2 to thromboxane A2 it influences the regulation of vasoconstriction and platelet aggregation.

Biological function summary

Thromboxane A2 generated by thromboxane synthase acts as a potent vasoconstrictor and promotes platelet aggregation important for hemostasis. This enzyme does not function as part of a larger complex but independently facilitates thromboxane A2 production. Its action significantly contributes to cardiovascular function where it modulates vascular tone and platelet activity.

Pathways

Thromboxane synthase is integral to the arachidonic acid metabolic pathway and the eicosanoid signaling pathway. The enzyme interacts closely with cyclooxygenase enzymes COX-1 and COX-2 in these pathways as they provide the prostaglandin H2 substrate for thromboxane A2 synthesis. By influencing these pathways thromboxane synthase indirectly affects inflammation and cardiovascular regulation.

The activity of thromboxane synthase is associated with cardiovascular diseases and disorders such as myocardial infarction and stroke. Overproduction of thromboxane A2 facilitated by this enzyme can lead to excessive platelet aggregation and vasoconstriction contributing to these conditions. Thromboxane synthase is also related to COX-2 which is implicated in inflammatory responses and is a target for anti-inflammatory drugs like aspirin and other COX inhibitors.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation (PubMed : 11097184, PubMed : 11297515, PubMed : 22735388, PubMed : 24009185, PubMed : 8436233, PubMed : 9873013). Also cleaves PGH2 to 12-hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2 (PubMed : 11297515, PubMed : 22735388, PubMed : 9873013). Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and 15-HETE (PubMed : 17459323).

See full target information TBXAS1

style

left-column

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

PloS one 11:e0151617 PubMed26974824

2016

Lack of Thromboxane Synthase Prevents Hypertension and Fetal Growth Restriction after High Salt Treatment during Pregnancy.

Applications

IHC

Species

Unspecified reactive species

Chen-Hsueh Pai,Ching-Tzu Yen,Chie-Pein Chen,I-Shing Yu,Shu-Wha Lin,Shu-Rung Lin

Atherosclerosis 246:98-105 PubMed26771386

2016

Vasoactive enzymes and blood flow responses to passive and active exercise in peripheral arterial disease.

Applications

Unspecified application

Species

Unspecified reactive species

Meegan A Walker,Birgitte Hoier,Philip J Walker,Karl Schulze,Jens Bangsbo,Ylva Hellsten,Christopher D Askew

The Journal of biological chemistry 287:6454-68 PubMed22219191

2012

Induction of COX-2 enzyme and down-regulation of COX-1 expression by lipopolysaccharide (LPS) control prostaglandin E2 production in astrocytes.

Applications

Species

Mouse

Miriam Font-Nieves,M Glòria Sans-Fons,Roser Gorina,Ester Bonfill-Teixidor,Angélica Salas-Pérdomo,Leonardo Márquez-Kisinousky,Tomàs Santalucia,Anna M Planas

American journal of physiology. Heart and circulatory physiology 302:H479-88 PubMed22037194

2011

Effects of cyclooxygenase inhibition on cardiovascular function in a hypercholesterolemic swine model of chronic ischemia.

Applications

Species

Pig

Louis M Chu,Michael P Robich,Cesario Bianchi,Jun Feng,Yuhong Liu,Shu-Hua Xu,Thomas Burgess,Frank W Sellke

Hypertension (Dallas, Tex. : 1979) 58:943-9 PubMed21896936

2011

Exercise training alters the balance between vasoactive compounds in skeletal muscle of individuals with essential hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Ane H Hansen,Michael Nyberg,Jens Bangsbo,Bengt Saltin,Ylva Hellsten

The Annals of thoracic surgery 92:829-36 PubMed21871266

2011

Thromboxane-induced contractile response of human coronary arterioles is diminished after cardioplegic arrest.

Applications

Species

Human

Jun Feng,Yuhong Liu,Louis M Chu,Richard T Clements,Kamal R Khabbaz,Michael P Robich,Cesario Bianchi,Frank W Sellke

Free radical biology & medicine 51:1686-96 PubMed21856412

2011

Inhibition of aldose reductase prevents endotoxin-induced inflammation by regulating the arachidonic acid pathway in murine macrophages.

Applications

Species

Mouse

Mohammad Shoeb,Umesh C S Yadav,Satish K Srivastava,Kota V Ramana

Inflammation research : official journal of the European Histamine Research Society ... [et al.] 60:87-92 PubMed20694740

2010

Thromboxane-prostanoid receptor expression and antagonism in dextran-sodium sulfate-induced colitis.

Applications

IHC-Fr

Species

Mouse

Patsy R Carter,Robert M McElhatten,Songlin Zhang,William S Wright,Norman R Harris

Experimental eye research 89:532-7 PubMed19523949

2009

Expression of thromboxane synthase and the thromboxane-prostanoid receptor in the mouse and rat retina.

Applications

IHC-P, IHC-P

Species

Rat, Mouse

William S Wright,Robert M McElhatten,Norman R Harris

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thromboxane synthase antibody

Western blot - Anti-Thromboxane synthase antibody (AB39362)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

Product promise