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AB249344

Anti-Thromboxane synthase antibody [EPR7333(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Thromboxane synthase antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

CYP5, CYP5A1, TXAS, TBXAS1, Thromboxane-A synthase, TXA synthase, TXS, Cytochrome P450 5A1, Hydroperoxy icosatetraenoate dehydratase

1 Images
Western blot - Anti-Thromboxane synthase antibody [EPR7333(2)] - BSA and Azide free (AB249344)
  • WB

Unknown

Western blot - Anti-Thromboxane synthase antibody [EPR7333(2)] - BSA and Azide free (AB249344)

This data was developed using ab157481, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Thromboxane synthase antibody [EPR7333(2)] (<a href='/en-us/products/primary-antibodies/thromboxane-synthase-antibody-epr73332-ab157481'>ab157481</a>) at 1/1000 dilution

Lane 1:

WI38 cell lysate at 10 µg

Lane 2:

Fetal liver lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 61 kDa

false

  • Unconjugated

    Anti-Thromboxane synthase antibody [EPR7333(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7333(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab249344 is the carrier-free version of ab157481.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thromboxane synthase also known as TBXAS1 is an enzyme that plays an important role in the biosynthesis of thromboxane A2 from prostaglandin H2. It is a 60 kDa protein primarily expressed in platelets and megakaryocytes as well as in cells within the lung kidney and spleen. By catalyzing the conversion of prostaglandin H2 to thromboxane A2 it influences the regulation of vasoconstriction and platelet aggregation.
Biological function summary

Thromboxane A2 generated by thromboxane synthase acts as a potent vasoconstrictor and promotes platelet aggregation important for hemostasis. This enzyme does not function as part of a larger complex but independently facilitates thromboxane A2 production. Its action significantly contributes to cardiovascular function where it modulates vascular tone and platelet activity.

Pathways

Thromboxane synthase is integral to the arachidonic acid metabolic pathway and the eicosanoid signaling pathway. The enzyme interacts closely with cyclooxygenase enzymes COX-1 and COX-2 in these pathways as they provide the prostaglandin H2 substrate for thromboxane A2 synthesis. By influencing these pathways thromboxane synthase indirectly affects inflammation and cardiovascular regulation.

The activity of thromboxane synthase is associated with cardiovascular diseases and disorders such as myocardial infarction and stroke. Overproduction of thromboxane A2 facilitated by this enzyme can lead to excessive platelet aggregation and vasoconstriction contributing to these conditions. Thromboxane synthase is also related to COX-2 which is implicated in inflammatory responses and is a target for anti-inflammatory drugs like aspirin and other COX inhibitors.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the conversion of prostaglandin H2 (PGH2) to thromboxane A2 (TXA2), a potent inducer of blood vessel constriction and platelet aggregation (PubMed : 11097184, PubMed : 11297515, PubMed : 22735388, PubMed : 24009185, PubMed : 8436233, PubMed : 9873013). Also cleaves PGH2 to 12-hydroxy-heptadecatrienoicacid (12-HHT) and malondialdehyde, which is known to act as a mediator of DNA damage. 12-HHT and malondialdehyde are formed stoichiometrically in the same amounts as TXA2 (PubMed : 11297515, PubMed : 22735388, PubMed : 9873013). Additionally, displays dehydratase activity, toward (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoate (15(S)-HPETE) producing 15-KETE and 15-HETE (PubMed : 17459323).
See full target information TBXAS1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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