AB101486

Anti-ThrRS antibody

Name: Anti-ThrRS antibody (ab101486) | Abcam
Brand: Abcam Limited
SKU: AB101486
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal ThrRS antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TARS1 aa 350-650.

View Alternative Names

TARS, TARS1, Threonyl-tRNA synthetase, Threonyl-tRNA synthetase 1, ThrRS

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB101486)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB101486)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labelling ThrRS protein at cytoplasm with ab101486 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB101486)

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labelling ThrRS protein at cytoplasm with ab101486 at a 1/500 dilution.

Antigen retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Supplier Data

Western blot - Anti-ThrRS antibody (AB101486)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-ThrRS antibody (ab101486) at 1/1000 dilution

Lane 1:

293T whole cell lysates at 30 µg

Lane 2:

A431 whole cell lysates at 30 µg

Lane 3:

HeLa whole cell lysates at 30 µg

Lane 4:

HepG2 whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 83 kDa

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Unknown

Western blot - Anti-ThrRS antibody (AB101486)

7.5% SDS PAGE

All lanes:

Western blot - Anti-ThrRS antibody (ab101486) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 45 kDa,54 kDa,56 kDa,61 kDa,69 kDa,72 kDa,78 kDa,83 kDa,88 kDa,92 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TARS1 aa 350-650. The exact immunogen used to generate this antibody is proprietary information.

P26639

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ThrRS also known as threonyl-tRNA synthetase is an enzyme that attaches the amino acid threonine to its corresponding tRNA (tRNA^Thr) an important step in the translation process during protein synthesis. ThrRS's molecular mass is approximately 80 kDa. It is expressed in various tissues across many organisms showing high conservation from bacteria to humans. This enzyme ensures accurate pairing of threonine with its tRNA which is critical for translating genetic information into functional proteins.

Biological function summary

Threonyl-tRNA synthetase plays a role in cellular function by ensuring proteins are synthesized accurately and efficiently. ThrRS is a standalone enzyme and does not form part of an enzyme complex. Its correct functioning is essential for maintaining cellular homeostasis and ensuring the fidelity of the genetic code. Incorrect aminoacylation by ThrRS can lead to translational errors which may manifest in malfunctioning proteins.

Pathways

The activity of threonyl-tRNA synthetase is integral to the protein synthesis pathway which is part of the larger biological process of gene expression. This pathway involves collaboration with other aminoacyl-tRNA synthetases and overlaps with translation mechanisms. ThrRS is related to proteins such as serine-tRNA synthetase and alanine-tRNA synthetase as they also participate in aminoacylation facilitating accurate protein synthesis by adding the right amino acids to tRNA molecules.

Problems associated with threonyl-tRNA synthetase can lead to conditions like Charcot-Marie-Tooth disease a disorder affecting peripheral nerves and certain types of cancer. Mutations in the ThrRS gene can alter its enzymatic activity or stability potentially disrupting the translation process. Moreover issues with ThrRS can interact with defective proteins like glycyl-tRNA synthetase further impacting cellular function and contributing to disease pathologies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the attachment of threonine to tRNA(Thr) in a two-step reaction : threonine is first activated by ATP to form Thr-AMP and then transferred to the acceptor end of tRNA(Thr) (PubMed : 25824639, PubMed : 31374204). Also edits incorrectly charged tRNA(Thr) via its editing domain, at the post-transfer stage (By similarity).

See full target information TARS1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of immunology (Baltimore, Md. : 1950) 197:296-302 PubMed27259858

2016

MMP-25 Metalloprotease Regulates Innate Immune Response through NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Clara Soria-Valles,Ana Gutiérrez-Fernández,Fernando G Osorio,Dido Carrero,Adolfo A Ferrando,Enrique Colado,M Soledad Fernández-García,Elena Bonzon-Kulichenko,Jesús Vázquez,Antonio Fueyo,Carlos López-Otín

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ThrRS antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB101486)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ThrRS antibody (AB101486)

Western blot - Anti-ThrRS antibody (AB101486)

Western blot - Anti-ThrRS antibody (AB101486)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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