AB249268

Anti-THSD1 antibody [EPR9506] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal THSD1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

TMTSP, UNQ3010/PRO9769, THSD1, Thrombospondin type-1 domain-containing protein 1, Transmembrane molecule with thrombospondin module

1 Images

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Western blot - Anti-THSD1 antibody [EPR9506] - BSA and Azide free (AB249268)

This data was developed using ab156573, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-THSD1 antibody [EPR9506] - BSA and Azide free (ab249268) at 1/1000 dilution

Lane 1:

HepG2 cell lysate at 10 µg/mL

Lane 2:

K562 cell lysate at 10 µg/mL

Lane 3:

U87-MG cell lysate at 10 µg/mL

Lane 4:

BxPC-3 cell lysate at 10 µg/mL

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 95 kDa

Observed band size: 48-95 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9506

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249268 is the carrier-free version of ab156573.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THSD1 also known as Thrombospondin Type 1 Domain Containing Protein 1 is a protein encoded by the THSD1 gene. It weighs approximately 75 kDa. THSD1 is expressed in various tissues including vascular endothelial cells and certain regions of the brain. This protein contains thrombospondin type 1 repeats that suggest its involvement in cell-matrix interactions and cellular adhesion processes.

Biological function summary

THSD1 plays a role in cell adhesion and migration. It is involved in blood vessel development and maintenance influencing angiogenesis. THSD1 interacts within the extracellular matrix and may form part of larger adhesion complexes though its exact partners in these complexes require further study. It affects cellular communication and structural organization by binding to other extracellular or membrane proteins.

Pathways

THSD1 participates in cellular adhesion and vascular development pathways. In the angiogenesis pathway it works alongside proteins like VEGF and integrins contributing to endothelial cell function and new blood vessel formation. It has ties to signaling pathways impacting endothelial cell survival and proliferation highlighting its significance in vascular biology.

Mutations or altered expression of THSD1 associate with hereditary hemorrhagic telangiectasia (HHT) and certain cancers including glioblastoma. It links with proteins like endoglin and ALK1 in the context of HHT where it may impact the vascular malformations characteristic of the disease. In cancer its role in cell adhesion and migration can influence tumor progression and metastasis.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Is a positive regulator of nascent focal adhesion assembly, involved in the modulation of endothelial cell attachment to the extracellular matrix.

See full target information THSD1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-THSD1 antibody [EPR9506] - BSA and Azide free

Western blot - Anti-THSD1 antibody [EPR9506] - BSA and Azide free (AB249268)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise