AB199850

Anti-THUMPD1 antibody

Name: Anti-THUMPD1 antibody (ab199850) | Abcam
Brand: Abcam Limited
SKU: AB199850
Price: 510 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal THUMPD1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human THUMPD1 aa 300 to C-terminus.

View Alternative Names

THUMP domain-containing protein 1, THUMPD1

2 Images

Supplier Data

Immunoprecipitation - Anti-THUMPD1 antibody (AB199850)

Detection of THUMPD1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab199850 at 6 μg/mg lysate for IP (Lane 1) and at 0.4 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-THUMPD1 antibody (ab199850)

Predicted band size: 39 kDa

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Supplier Data

Western blot - Anti-THUMPD1 antibody (AB199850)

Lysates prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-THUMPD1 antibody (ab199850) at 0.1 µg/mL

Lane 1:

HeLa cell lysate at 50 µg

Lane 2:

293T cell lysate at 50 µg

Lane 3:

Jurkat cell lysate at 50 µg

Predicted band size: 39 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human THUMPD1 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9NXG2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

THUMPD1 also known as THUMP domain-containing protein 1 is a protein involved in RNA modification processes. The protein has a molecular mass of approximately 45 kDa. THUMPD1 expresses in various tissues with notable levels in the brain liver and testis. As an essential protein it plays a mechanical role in RNA processing and modification specifically within transfer RNAs.

Biological function summary

THUMPD1 is involved in the methylation of RNA molecules. It often functions as part of a larger protein complex collaborating with other proteins for accurate RNA modification. These modifications affect RNA stability and translation critical for regular cellular function. This active participation in RNA systems indicates a significant impact on maintaining protein homeostasis within cells.

Pathways

THUMPD1 has a role in important RNA-centric pathways. It connects with the tRNA methylation pathway and is associated closely with the RNA processing pathway. Proteins like TRMT1 and NOP2 relate to THUMPD1 within these pathways sharing the common function of ensuring correct RNA modifications. This connectivity positions THUMPD1 as a significant participant in genetic regulation activities.

THUMPD1 links to several genetic conditions with defects in RNA modification pathways. Research highlights connections to specific cancers such as liver cancer where dysregulation of RNA methylation is observed. Additionally THUMPD1 may influence neurological disorders through related pathways. Interactions with proteins such as METTL3 further suggest its involvement in these conditions where changes in RNA processing are a factor.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Functions as a tRNA-binding adapter to mediate NAT10-dependent tRNA acetylation modifying cytidine to N4-acetylcytidine (ac4C) (PubMed : 25653167, PubMed : 35196516).

See full target information THUMPD1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cancer therapeutics 23:1743-1760 PubMed39210605

2024

Immunoproteasome Activation Expands the MHC Class I Immunopeptidome, Unmasks Neoantigens, and Enhances T-cell Anti-Myeloma Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Priyanka S Rana,James J Ignatz-Hoover,Chunna Guo,Amber L Mosley,Ehsan Malek,Yuriy Federov,Drew J Adams,James J Driscoll

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-THUMPD1 antibody

Immunoprecipitation - Anti-THUMPD1 antibody (AB199850)

Western blot - Anti-THUMPD1 antibody (AB199850)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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