AB239509

Anti-Thymidine Kinase 1/TK1 antibody [C1]

Name: Anti-Thymidine Kinase 1/TK1 antibody [C1] (ab239509) | Abcam
Brand: Abcam Limited
SKU: AB239509

Be the first to review this product! Submit a review

(2 Publications)

Mouse Monoclonal Thymidine Kinase 1/TK1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TK1.

View Alternative Names

TK1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

Paraffin-embedded human uterine fibroids tissue stained for Thymidine Kinase 1/TK1 using ab239509 at 30 μg/ml in immunohistochemical analysis. DAB staining.

Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

MCF7 (human breast adenocarcinoma cell line) cells stained for Thymidine Kinase 1/TK1 (green) using ab239509 at 30 μg/ml in ICC/IF, followed by FITC staining.

Supplier Data

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

All lanes:

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (ab239509) at 6 µg/mL

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Predicted band size: 25 kDa

false

Supplier Data

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

All lanes:

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (ab239509) at 5 µg/mL

All lanes:

Recombinant human Thymidine Kinase 1/TK1 protein

Predicted band size: 25 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TK1.

P04183

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5-30 µg/mL", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5-30 µg/mL", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thymidine Kinase 1 (TK1) also known as Thymidine Kinase isoform A is an enzyme involved in the phosphorylation of thymidine into thymidine monophosphate an important component in DNA synthesis. TK1 has a molecular weight of approximately 25 kDa. It is predominantly expressed in proliferating cells with expression levels peaking during the S phase of the cell cycle. You often find TK1 in tissues with high cell division rates like bone marrow and lymphatic tissues.

Biological function summary

This enzyme is critical for DNA replication and repair by providing deoxythymidine triphosphate (dTTP) a deoxynucleoside triphosphate. TK1 operates as a monomer but can form a tetrameric complex in its active state which enhances its affinity for substrates. TK1 can process various substrates including thymidine and thymidine analogs such as 5-bromo-2'-deoxyuridine (BrdU) and EdU. These substrates are vital in molecular biology as they help in measuring DNA synthesis in cell proliferation studies.

Pathways

The function of TK1 is central to the salvage pathway of nucleotide synthesis. This pathway allows recycling of thymidine from degraded DNA which is particularly important for rapidly dividing cells that need to ensure a sufficient supply of nucleotides. TK1 acts alongside other enzymes such as thymidylate synthase and ribonucleotide reductase to maintain nucleotide balance within the cell ensuring proper DNA synthesis and cellular proliferation.

TK1 levels often increase in cancerous tissues due to their elevated cell proliferation rates. Serum TK1 is a biomarker for various cancers like leukemia and breast cancer. Studies show a correlation between high TK1 activity and tumor aggressiveness and progression. Additionally interactions of TK1 with the protein p53 a tumor suppressor show that defects in TK1 regulation can contribute to genomic instability highlighting its role in oncogenesis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cell-cycle-regulated enzyme of importance in nucleotide metabolism (PubMed : 9575153). Catalyzes the first enzymatic step in the salvage pathway converting thymidine into thymidine monophosphate (PubMed : 22385435). Transcriptional regulation limits expression to the S phase of the cell cycle and transient expression coincides with the oscillation in the intracellular dTTP concentration (Probable). Also important for the activation of anticancer and antiviral nucleoside analog prodrugs such as 1-b-d-arabinofuranosylcytosine (AraC) and 3c-azido-3c-deoxythymidine (AZT) (PubMed : 22385435).

See full target information TK1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:61 PubMed39806464

2025

The microenvironment cell index is a novel indicator for the prognosis and therapeutic regimen selection of cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Xian-Yan Yang,Nian Chen,Qian Wen,Yu Zhou,Tao Zhang,Ji Zhou,Cheng-Hui Liang,Li-Ping Han,Xiao-Ya Wang,Qing-Mei Kang,Xiao-Xia Zheng,Xue-Jia Zhai,Hong-Ying Jiang,Tian-Hua Shen,Jin-Wei Xiao,Yu-Xin Zou,Yun Deng,Shuang Lin,Jiang-Jie Duan,Jun Wang,Shi-Cang Yu

Frontiers in molecular biosciences 9:848463 PubMed35480888

2022

Identification of Novel Immune Cell-Relevant Therapeutic Targets and Validation of Roles of TK1 in BMSCs of Systemic Lupus Erythematosus.

Applications

Unspecified application

Species

Unspecified reactive species

Fangru Chen,Jian Meng,Wenjie Yan,Mengjiao Wang,Yunfei Jiang,Jintao Gao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thymidine Kinase 1/TK1 antibody [C1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

Immunocytochemistry/ Immunofluorescence - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

Western blot - Anti-Thymidine Kinase 1/TK1 antibody [C1] (AB239509)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise