AB270728

Anti-Thymidine Phosphorylase antibody [rTYMP/3444]

Recombinant
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(1 Publication)

Mouse Recombinant Monoclonal Thymidine Phosphorylase antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TYMP.

View Alternative Names

ECGF1, TYMP, Thymidine phosphorylase, TP, Gliostatin, Platelet-derived endothelial cell growth factor, TdRPase, PD-ECGF

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [rTYMP/3444] (AB270728)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [rTYMP/3444] (AB270728)

Formalin-fixed, paraffin-embedded human placenta tissue stained for thymidine phosphorylase using ab270728 at 2 μg/ml in immunohistochemical analysis.

Supplier Data

Western blot - Anti-Thymidine Phosphorylase antibody [rTYMP/3444] (AB270728)

All lanes:

Western blot - Anti-Thymidine Phosphorylase antibody [rTYMP/3444] (ab270728)

All lanes:

Human spleen lysate

Predicted band size: 50 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

rTYMP/3444

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TYMP.

P19971

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from Bioreactor Concentrate.

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thymidine phosphorylase also known as platelet-derived endothelial cell growth factor (PD-ECGF) acts mechanically to catalyze the reversible phosphorolysis of thymidine into thymine and 2-deoxy-alpha-D-ribose-1-phosphate. With a mass of approximately 44 kDa this enzyme is present in various tissues including blood vessels and macrophages. Its expression tends to be higher in tumor tissues which suggests a specific role in cancer biology.

Biological function summary

The enzyme participates in pyrimidine nucleoside metabolism providing an alternate route for thymidine catabolism. It is not part of a larger complex functioning independently to promote angiogenesis. Thymidine phosphorylase enhances the degradation of extracellular thymidine which can positively impact nucleotide balance within cells aiding DNA synthesis and repair.

Pathways

Thymidine phosphorylase operates significantly within the nucleotide salvage pathways. It is essential for clarifying thymidine availability for DNA synthesis. The enzyme links with enzymes like dihydropyrimidine dehydrogenase in pyrimidine degradation playing a critical role in maintaining cellular nucleotide homeostasis.

Altered activity of thymidine phosphorylase correlates with cancer and mitochondrial neurogastrointestinal encephalomyopathy (MNGIE). In tumors increased levels of this enzyme facilitate angiogenesis contributing to tumor growth and metastasis. In MNGIE mutations in this enzyme lead to imbalances in nucleotides causing mitochondrial dysfunction. The enzyme's dysregulation connects to angiogenesis-related proteins like VEGF in cancer highlighting potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

May have a role in maintaining the integrity of the blood vessels. Has growth promoting activity on endothelial cells, angiogenic activity in vivo and chemotactic activity on endothelial cells in vitro.. Catalyzes the reversible phosphorolysis of thymidine. The produced molecules are then utilized as carbon and energy sources or in the rescue of pyrimidine bases for nucleotide synthesis.

See full target information TYMP

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of chronic obstructive pulmonary disease 20:551-564 PubMed40078929

2025

PGAM5 Modulates Macrophage Polarization, Aggravating Inflammation in COPD via the NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Zheng,Yujie Wang,Jia Li,Shaomao Zheng,Lipeng Zhang,Qiaoyu Li,Fayu Ling,Qiuli Nie,Qiong Feng,Jing Wang,Chengji Jin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thymidine Phosphorylase antibody [rTYMP/3444]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidine Phosphorylase antibody [rTYMP/3444] (AB270728)

Western blot - Anti-Thymidine Phosphorylase antibody [rTYMP/3444] (AB270728)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise