AB7398

Anti-Thymidylate Synthase antibody

Name: Anti-Thymidylate Synthase antibody (ab7398) | Abcam
Brand: Abcam Limited
SKU: AB7398

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(7 Publications)

Sheep Polyclonal Thymidylate Synthase antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TYMS.

View Alternative Names

TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase

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Western blot - Anti-Thymidylate Synthase antibody (AB7398)

ab7398 is shown to detect thymidylate synthase present in a HeLa cell extract. Each lane is estimated to contain 4 µg of protein. Lanes 1, 2 and 3 represent 1/2000, 1/5000 and 1/10000 fold dilutions of the antibody. Detection was made using HRP Rabbit-a-Sheep IgG diluted 1/1000 and color development using TMB substrate for approximately 4'.

All lanes:

Western blot - Anti-Thymidylate Synthase antibody (ab7398)

Predicted band size: 36 kDa

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Key facts

Host species

Sheep

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TYMS.

P04818

Specificity

In general, this antibody can detect antigen in a variety of human cells and tissues, as well as bacteria, African green monkey, rat and mouse. Somewhat lower dilutions may be required in some non-human cell lines. Anti-Thymidylate Synthase can detect Thymidylate Synthase by immunochemistry in proliferating cell cultures and tissues but does not stain nonproliferating cells. Normal colon mucosa shows weak staining; however, some colorectal cancer specimens show very strong staining.

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Reactivity data

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Product details

The antibody recognizes the expected additional band corresponding to the ternary complex of human TS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR. This event occurs in most human breast, colorectal, gastric, head and neck carcinomas. The antibody recognizes 36 kDa human TS.

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.

Biological function summary

Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.

Pathways

Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.

Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the mitochondrial and nuclear de novo thymidylate biosynthesis pathway.

See full target information TYMS

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

EBioMedicine 43:473-486 PubMed31060905

2019

Disrupted folate metabolism with anesthesia leads to myelination deficits mediated by epigenetic regulation of ERMN.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Zhenyu Xue,Qidong Liu,Yunbo Liu,Siwei Xi,Yanyong Cheng,Jingjie Li,Jia Yan,Yuan Shen,Chong Xiao,Zhongcong Xie,Zilong Qiu,Hong Jiang

Oncotarget 6:33658-74 PubMed26378040

2015

GRP78 confers the resistance to 5-FU by activating the c-Src/LSF/TS axis in hepatocellular carcinoma.

Applications

Species

Unspecified reactive species

Yan-jiao Gu,Hong-dan Li,Liang Zhao,Song Zhao,Wu-bin He,Li Rui,Chang Su,Hua-chuan Zheng,Rong-jian Su

The Journal of nutrition 144:419-24 PubMed24500934

2014

Azoxymethane-induced colon carcinogenesis in mice occurs independently of de novo thymidylate synthesis capacity.

Applications

Unspecified application

Species

Unspecified reactive species

Amanda J MacFarlane,Michael F McEntee,Patrick J Stover

The Journal of biological chemistry 286:44015-22 PubMed22057276

2011

Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA.

Applications

Species

Mouse

Amanda J MacFarlane,Donald D Anderson,Per Flodby,Cheryll A Perry,Robert H Allen,Sally P Stabler,Patrick J Stover

EMBO reports 11:698-704 PubMed20671737

2010

Nucleotide supply, not local histone acetylation, sets replication origin usage in transcribed regions.

Applications

Species

Chinese hamster

Sophie Gay,Anne-Marie Lachages,Gael A Millot,Sylvain Courbet,Anne Letessier,Michelle Debatisse,Olivier Brison

The Journal of biological chemistry 284:31097-108 PubMed19734144

2009

A UV-responsive internal ribosome entry site enhances serine hydroxymethyltransferase 1 expression for DNA damage repair.

Applications

WB, IP

Species

Human, Human

Jennifer T Fox,William K Shin,Marie A Caudill,Patrick J Stover

The Journal of biological chemistry 282:17623-31 PubMed17446168

2007

Evidence for small ubiquitin-like modifier-dependent nuclear import of the thymidylate biosynthesis pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Collynn F Woeller,Donald D Anderson,Doletha M E Szebenyi,Patrick J Stover

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Thymidylate Synthase antibody

Western blot - Anti-Thymidylate Synthase antibody (AB7398)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise