Rabbit Recombinant Monoclonal Thymidylate Synthase antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 18 publications.
Images
![Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--immunoprecipitation-img3303.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)")
![Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--western-blot-img29850.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)")
![Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--flow-cytometry-intracellular-img38666.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)")
![Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--immunocytochemistry-immunofluorescence-img69629.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119463.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)")
Publications
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- Nature communications 13:13802022Indisulam targets RNA splicing and metabolism to serve as a therapeutic strategy for high-risk neuroblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnke Nijhuis,Arti Sikka,Orli Yogev,Lili Herendi,Cristina Balcells,Yurui Ma,Evon Poon,Clare Eckold,Gabriel N Valbuena,Yuewei Xu,Yusong Liu,Barbara Martins da Costa,Michael Gruet,Chiharu Wickremesinghe,Adrian Benito,Holger Kramer,Alex Montoya,David Carling,Elizabeth J Want,Yann Jamin,Louis Chesler,Hector C KeunPubMed 35296644
- Frontiers in genetics 12:7750062021Identification of Potential Hub Genes and miRNA-mRNA Pairs Related to the Progression and Prognosis of Cervical Cancer Through Integrated Bioinformatics Analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingxu Fu,Yongyan Pei,Fang Lu,Huici Jiang,Yingying Bi,Jiajing Cheng,Jinlong QinPubMed 35003215
- Frontiers in pharmacology 12:7186752021Upregulation of Thymidylate Synthase Induces Pemetrexed Resistance in Malignant Pleural Mesothelioma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuzo Sato,Masaru Tomita,Tomoyoshi Soga,Atsushi Ochiai,Hideki MakinoshimaPubMed 34646134
- Pathology international 71:752-7642021Expression of astrocyte-elevated gene-1 indicates prognostic value of fluoropyrimidine-based adjuvant chemotherapy in resectable stage III colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLong-Wei Lin,Peng-Sheng Lai,Ying-Yin Chen,Chung-Yu ChenPubMed 34528330
- Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 24:823-8342021Tumor mutation burden as a biomarker in resected gastric cancer via its association with immune infiltration and hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDeqiang Wang,Ning Wang,Xiaoqin Li,Xiaofeng Chen,Bo Shen,Dongqin Zhu,Liuqing Zhu,Yaping Xu,Yangyang Yu,Yongqian ShuPubMed 33687617
- Frontiers in immunology 11:6093372021Tumor Microenvironment Status Predicts the Efficacy of Postoperative Chemotherapy or Radiochemotherapy in Resected Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRan Duan,Xiaoqin Li,Dongqiang Zeng,Xiaofeng Chen,Bo Shen,Dongqin Zhu,Liuqing Zhu,Yangyang Yu,Deqiang WangPubMed 33569057
- Molecular cancer therapeutics 20:64-752020Reprogramming of Nucleotide Metabolism Mediates Synergy between Epigenetic Therapy and MAP Kinase Inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatiana Shorstova,Jie Su,Tiejun Zhao,Michael Dahabieh,Matthew Leibovitch,Mariana De Sa Tavares Russo,Daina Avizonis,Shivshankari Rajkumar,Ian R Watson,Sonia V Del Rincón,Wilson H Miller,William D Foulkes,Michael WitcherPubMed 33087508
- Oncology reports 44:565-5762020Identification of TYMS as a promoting factor of retroperitoneal liposarcoma progression: Bioinformatics analysis and biological evidence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSha Zhang et. al.PubMed 32627015
- Frontiers in oncology 10:5302020Perineural Invasion and Postoperative Adjuvant Chemotherapy Efficacy in Patients With Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQing Tao et. al.PubMed 32373527
- Cell death & disease 10:3272019The mevalonate coordinates energy input and cell proliferation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi Gong,Yi Xiao,Fan Xia,Pei Wu,Tingting Zhao,Shulin Xie,Ran Wang,Qiaocheng Wen,Wensu Zhou,Huilan Xu,Lingyan Zhu,Zeqi Zheng,Tianlun Yang,Zihua Chen,Qiong DuanPubMed 30975976
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested | Expected | Expected |
| Rat | Expected | Expected | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Heat up to 98°C, below boiling, and then let cool for 10-20 minutes. The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
Target data
Function
Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the de novo mitochondrial thymidylate biosynthesis pathway.
Alternative names
TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase
Recommended products
Rabbit Recombinant Monoclonal Thymidylate Synthase antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 18 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR4545
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.
- Biological function summary
Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.
- Pathways
Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.
- Associated diseases and disorders
Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--immunoprecipitation-img3303.jpg "Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)")
Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
ab108995 (purified) at 1:30 dilution (2μg) immunoprecipitating Thymidylate Synthase in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate,10μg
Lane 2 (+): ab108995 & Jurkat whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab108995 in Jurkat whole cell lysate
For western blotting, VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1:1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.All lanes: Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
Predicted band size: 36 kDa
![Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--western-blot-img29850.jpg "Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)")
Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/10000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2: Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg
Lane 3: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
Lane 4: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 36 kDa, 77 kDa
Observed band size: 77 kDa
![Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--flow-cytometry-intracellular-img38666.jpg "Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)")
Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Thymidylate Synthase with purified ab108995 at 1/50 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--immunocytochemistry-immunofluorescence-img69629.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)")
Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Thymidylate Synthas with Purified ab108995 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119463.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling Thymidylate Synthase with purified ab108995 at 1:100 dilution. Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
![Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--western-blot-img116051.jpg "Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)")
Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
All lanes: Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Jurkat cell lysate at 10 µg
Lane 3: HL60 cell lysate at 10 µg
Lane 4: MCF7 cell lysate at 10 µg
Performed under reducing conditions.
Predicted band size: 36 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img70915.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)
Unpurified ab108995 at 1/50 dilution, staining Thymidylate Synthase in Human ovary carcinoma Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995), expandable thumbnail](https://content.abcam.com/products/images/thymidylate-synthase-antibody-epr4545-ab108995--western-blot-img116051.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)")
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