AB108995

Anti-Thymidylate Synthase antibody [EPR4545]

Name: Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) | Abcam
Brand: Abcam Limited
SKU: AB108995
Price: 365 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(25 Publications)

Rabbit Recombinant Monoclonal Thymidylate Synthase antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 25 publications.

View Alternative Names

TS, OK/SW-cl.29, TYMS, Thymidylate synthase, TSase

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Unpurified ab108995 at 1/50 dilution, staining Thymidylate Synthase in Human ovary carcinoma Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Thymidylate Synthas with Purified ab108995 at 1 : 100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100 ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue sections labeling Thymidylate Synthase with purified ab108995 at 1 : 100 dilution. Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Thymidylate Synthase with purified ab108995 at 1/50 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor^® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Lab

Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

ab108995 (purified) at 1 : 30 dilution (2μg) immunoprecipitating Thymidylate Synthase in Jurkat whole cell lysate.

Lane 1 (input) : Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate,10μg
Lane 2 (+) : ab108995 & Jurkat whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab108995 in Jurkat whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995)

Predicted band size: 36 kDa

false

Lab

Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

Jurkat (Human T cell leukemia T lymphocyte) whole cell lysates at 20 µg

Lane 3:

C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg

Lane 4:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 36 kDa,77 kDa

Observed band size: 77 kDa

false

Unknown

Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

All lanes:

Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (ab108995) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

HL60 cell lysate at 10 µg

Lane 4:

MCF7 cell lysate at 10 µg

Predicted band size: 36 kDa

false

Carrier free

Anti-Thymidylate Synthase antibody [EPR4545] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Thymidylate Synthase antibody [EPR4545]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-Thymidylate Synthase antibody [EPR4545]
660 APC

APC Anti-Thymidylate Synthase antibody [EPR4545]
578 PE

PE Anti-Thymidylate Synthase antibody [EPR4545]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4545

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "Heat up to 98°C, below boiling, and then let cool for 10-20 minutes. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10 - 1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Thymidylate Synthase (TS) also known as TYMS or TSase is an important enzyme involved in the de novo synthesis of thymidylate a nucleotide necessary for DNA replication and repair. TS catalyzes the methylation of deoxyuridine monophosphate (dUMP) to deoxythymidine monophosphate (dTMP) using 510-methylenetetrahydrofolate as a cofactor. It has an approximate molecular weight of 36 kDa. The enzyme is expressed in the cytoplasm of proliferating cells predominantly in tissues with high cellular turnover like bone marrow and the intestinal lining.

Biological function summary

Thymidylate synthase plays a critical role in DNA synthesis and cell division. As a member of the folate cycle it indirectly influences the supply of thymidine for DNA synthesis affecting cell proliferation. The enzyme generally functions as a homodimer which is necessary for its activity. Increased expression of TS usually correlates with high rates of cell division making it an important factor in rapidly growing tissues and tumors.

Pathways

Several important biological networks involve thymidylate synthase especially the folate and pyrimidine metabolism pathways. These pathways are essential for the maintenance of nucleotide pools within the cell. TS is functionally related to proteins like dihydrofolate reductase (DHFR) which also play roles in folate metabolism and DNA synthesis. Collectively these proteins ensure the proper balance of nucleotide precursors for DNA replication and repair.

Thymidylate synthase is closely associated with cancer and certain autoimmunity conditions. Overexpression of TS is commonly observed in various cancers as tumor cells require increased nucleotide synthesis for rapid proliferation. This makes TS a target for several anticancer drugs like 5-fluorouracil which inhibits TS activity. Additionally aberrations in enzymes like dihydropyrimidine dehydrogenase (DPD) could affect TS function leading to chemotherapeutic agent sensitivity in cancer treatments.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the reductive methylation of 2'-deoxyuridine 5'-monophosphate (dUMP) to thymidine 5'-monophosphate (dTMP), using the cosubstrate, 5,10- methylenetetrahydrofolate (CH2H4folate) as a 1-carbon donor and reductant and contributes to the mitochondrial and nuclear de novo thymidylate biosynthesis pathway.

See full target information TYMS

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1600863 PubMed40642061

2025

Revealing the significance of tissue-resident memory T cells in lung adenocarcinoma through bioinformatic analysis and experimental validation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuoqi Li,Mei Tian,Yuanhui Yang,Yuanyuan Wang,Lu Zhang,Fujing Huang,Xiao Wen,Xiaoshu Yin,Xiaoyan Lin,Yuan Tian

Chinese herbal medicines 17:127-138 PubMed39949800

2025

Material basis revelation of anti-hepatoma effect of Huachansu (Cinobufacini) through down-regulation of thymidylate synthase.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Wu,Qimei Chen,Jingyi Yang,Jiayu Zhang,Ailin Yang

American journal of translational research 16:5228-5247 PubMed39544745

2024

Unveiling the oncogenic significance of thymidylate synthase in human cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Yibo Geng,Luyang Xie,Yang Wang,Yan Wang

Cell chemical biology 31:221-233.e14 PubMed37875111

2023

Methotrexate-based PROTACs as DHFR-specific chemical probes.

Applications

Unspecified application

Species

Unspecified reactive species

Sandeep Rana,Patricia Dranchak,Jayme L Dahlin,Laurence Lamy,Wenqing Li,Erin Oliphant,Jonathan H Shrimp,Girish H Rajacharya,Ravi Tharakan,David O Holland,Apryl S Whitten,Kelli M Wilson,Pankaj K Singh,Scott K Durum,Dingyin Tao,Ganesha Rai,James Inglese

Nature communications 13:1380 PubMed35296644

2022

Indisulam targets RNA splicing and metabolism to serve as a therapeutic strategy for high-risk neuroblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Anke Nijhuis,Arti Sikka,Orli Yogev,Lili Herendi,Cristina Balcells,Yurui Ma,Evon Poon,Clare Eckold,Gabriel N Valbuena,Yuewei Xu,Yusong Liu,Barbara Martins da Costa,Michael Gruet,Chiharu Wickremesinghe,Adrian Benito,Holger Kramer,Alex Montoya,David Carling,Elizabeth J Want,Yann Jamin,Louis Chesler,Hector C Keun

Frontiers in genetics 12:775006 PubMed35003215

2021

Identification of Potential Hub Genes and miRNA-mRNA Pairs Related to the Progression and Prognosis of Cervical Cancer Through Integrated Bioinformatics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Mingxu Fu,Yongyan Pei,Fang Lu,Huici Jiang,Yingying Bi,Jiajing Cheng,Jinlong Qin

Bioengineered 12:11959-11977 PubMed34818974

2021

MicroRNA-140-3p represses the proliferation, migration, invasion and angiogenesis of lung adenocarcinoma cells via targeting TYMS (thymidylate synthetase).

Applications

Unspecified application

Species

Unspecified reactive species

Shanzhi Wan,Zhimin Liu,Yang Chen,Zhitao Mai,Mingming Jiang,Qingguo Di,Baohua Sun

Frontiers in pharmacology 12:718675 PubMed34646134

2021

Upregulation of Thymidylate Synthase Induces Pemetrexed Resistance in Malignant Pleural Mesothelioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuzo Sato,Masaru Tomita,Tomoyoshi Soga,Atsushi Ochiai,Hideki Makinoshima

Pathology international 71:752-764 PubMed34528330

2021

Expression of astrocyte-elevated gene-1 indicates prognostic value of fluoropyrimidine-based adjuvant chemotherapy in resectable stage III colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Long-Wei Lin,Peng-Sheng Lai,Ying-Yin Chen,Chung-Yu Chen

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 24:823-834 PubMed33687617

2021

Tumor mutation burden as a biomarker in resected gastric cancer via its association with immune infiltration and hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Deqiang Wang,Ning Wang,Xiaoqin Li,Xiaofeng Chen,Bo Shen,Dongqin Zhu,Liuqing Zhu,Yaping Xu,Yangyang Yu,Yongqian Shu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Thymidylate Synthase antibody [EPR4545]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Immunocytochemistry/ Immunofluorescence - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Flow Cytometry (Intracellular) - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Immunoprecipitation - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

Western blot - Anti-Thymidylate Synthase antibody [EPR4545] (AB108995)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

Product promise